Mechanisms of sperm chemotaxis
Abstract
Sperm are attracted by chemical factors that are released by the egg-a process called chemotaxis. Most of our knowledge on sperm chemotaxis originates from the study of marine invertebrates. In recent years, the main features of the chemotactic signaling pathway and the swimming behavior evoked by chemoattractants have been elucidated in sea urchins. In contrast, our understanding of mammalian sperm chemotaxis is still rudimentary and subject to an ongoing debate. In this review, we raise new questions and discuss current concepts of sperm chemotaxis. Finally, we highlight commonalities and differences of sensory signaling in sperm, photoreceptors, and olfactory neurons.
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Various single-celled eukaryotes (protists) and ciliated larvae of sponges devoid of neurons can display sophisticated behaviours, including phototaxis, gravitaxis or chemotaxis.
“Various single-celled eukaryotes (protists) and the ciliated larvae of sponges devoid of neurons can display sophisticated behaviours, including phototaxis, gravitaxis or chemotaxis.”
From Origin and early evolution of neural circuits for the control of ciliary locomotion (2011) · cites this paper as [31]
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Evidence that human follicular fluid contains a chemoattractant for spermatozoa
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Novel function of β-arrestin2 in the nucleus of mature spermatozoa
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The Production of Sperm Iso-Agglutinins by Ova
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Patterns of [Ca2+]i mobilization and cell response in human spermatozoa exposed to progesterone
Bedu-Addo, K., Barratt, C. L. R., Kirkman‐Brown, J., & Publicover, S. J. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.09.040 · Cited by 61 · see citing works
Galindo, B. E., Beltrán, C., Cragoe, E. J., & Darszon, A. (2000) · Developmental Biology · doi:10.1006/dbio.2000.9678 · Cited by 60 · see citing works
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Classic and Non-classic Progesterone Receptors are both Expressed in Human Spermatozoa
Lösel, R., Breiter, S., Seyfert, M., Wehling, M., & Falkenstein, E. (2005) · Hormone and Metabolic Research · doi:10.1055/s-2005-861023 · Cited by 56 · see citing works
The effect of RANTES on human sperm chemotaxis
Isobe, T., Minoura, H., Tanaka, K., Shibahara, T., Hayashi, N., & Toyoda, N. (2002) · Human Reproduction · doi:10.1093/humrep/17.6.1441 · Cited by 56 · see citing works
Linardopoulou, E. V. (2001) · Human Molecular Genetics · doi:10.1093/hmg/10.21.2373 · Cited by 56 · see citing works
Sp-tetraKCNG: A novel cyclic nucleotide gated K+ channel
Galindo, B. E., de la Vega‐Beltrán, J. L., Labarca, P. P., Vacquier, V. D., & Darszon, A. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.01.035 · Cited by 52 · see citing works
Fertilization in Sepia officinalis: the first mollusk sperm-attracting peptide
Zatylny‐Gaudin, C., Marvin, L., Gagnon, J., & Henry, J. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)02036-3 · Cited by 51 · see citing works
Schlichter, R., Keller, A. F., Roo, M. D., Breton, J., Inquimbert, P., & Poisbeau, P. (2006) · Journal of Molecular Neuroscience · doi:10.1385/jmn:28:1:33 · Cited by 50 · see citing works
Miraglia, E., Rullo, M. L., Bosìa, A., Massobrio, M., Revelli, A., & Ghigo, D. (2007) · Fertility and Sterility · doi:10.1016/j.fertnstert.2006.07.1540 · Cited by 47 · see citing works
Jaiswal, B. S., & Eisenbach, M. (2002) · Elsevier eBooks · doi:10.1016/b978-012311629-1/50005-x · Cited by 47 · see citing works
Identification of voltage‐dependent Ca2+ channels in sea urchin sperm
Granados‐González, G., Mendoza-Lujambio, I., Rodriguez, E., Galindo, B. E., Beltrán, C., & Darszon, A. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.10.035 · Cited by 46 · see citing works
Receptors in Spermatozoa: Are They Real?
Naz, R. K., & Sellamuthu, R. (2006) · Journal of Andrology · doi:10.2164/jandrol.106.000620 · Cited by 46 · see citing works
Lack of species-specificity in mammalian sperm chemotaxis
Sun, F., Giojalas, L. C., Rovasio, R. A., Tur-Kaspa, I., Sánchez, R., & Eisenbach, M. (2003) · Developmental Biology · doi:10.1016/s0012-1606(02)00090-8 · Cited by 44 · see citing works
Chemotactic behavior of the sperm of chitons (Mollusca: Polyplacophora)
Miller, R. L. (1977) · Journal of Experimental Zoology · doi:10.1002/jez.1402020209 · Cited by 44 · see citing works
Wang, Y., Storeng, R., Dale, P. O., Åbyholm, T., & Tanbo, T. G. (2001) · Gynecological Endocrinology · doi:10.1080/gye.15.4.286.292 · Cited by 42 · see citing works
Non-Genomic Steroid Actions in Human Spermatozoa
Correia, J., Conner, S. J., & Kirkman‐Brown, J. (2007) · Seminars in Reproductive Medicine · doi:10.1055/s-2007-973433 · Cited by 41 · see citing works
Signaling Pathways for Modulation of Mouse Sperm Motility by Adenosine and Catecholamine Agonists1
Schuh-Huerta, S. M., Carlson, A. E., McKnight, G. S., Conti, M., Hille, B., & Babcock, D. F. (2005) · Biology of Reproduction · doi:10.1095/biolreprod.105.047837 · Cited by 39 · see citing works
Expression of the G-protein α-subunit gustducin in mammalian spermatozoa
Fehr, J., Meyer, D., Widmayer, P., Borth, H., Ackermann, F., Wilhelm, B., Gudermann, T. W., & Boekhoff, I. (2006) · Journal of Comparative Physiology A · doi:10.1007/s00359-006-0168-8 · Cited by 38 · see citing works
A new hyperpolarization-activated, cyclic nucleotide-gated channel from sea urchin sperm flagella
Galindo, B. E., Neill, A. T., & Vacquier, V. D. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.06.074 · Cited by 38 · see citing works
Beta‐chemokine receptors 5 and 3 are expressed on the head region of human spermatozoon
Muciaccia, B., Padula, F., Vicini, E., Gandini, L., Lenzi, A., & Stefanini, M. (2005) · The FASEB Journal · doi:10.1096/fj.05-3962fje · Cited by 32 · see citing works
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Sperm-Activating Peptide Induces Asymmetric Flagellar Bending in Sea Urchin Sperm
Shiba, K., Ohmuro, J., Mogami, Y., Nishigaki, T., Wood, C. D., Darszon, A., Tatsu, Y., Yumoto, N., & Baba, S. A. (2005) · ZOOLOGICAL SCIENCE · doi:10.2108/zsj.22.293 · Cited by 28 · see citing works
Revisiting the Role of H+ in Chemotactic Signaling of Sperm
Solzin, J., Helbig, A., Van, Q., Brown, J. E., Hildebrand, E., Weyand, I., & Kaupp, U. B. (2004) · The Journal of General Physiology · doi:10.1085/jgp.200409030 · Cited by 28 · see citing works
Molecular mechanisms during sperm capacitation
Brewis, I. A., Moore, H. D. M., Fraser, L. R., Holt, W. V., Baldi, E., Luconi, M., Gadella, B. M., Ford, W. C. L., & Harrison, R. A. P. (2005) · Human Fertility · doi:10.1080/14647270500420178 · Cited by 26 · see citing works
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Adenylyl cyclase isoforms in rat testis and spermatozoa from the cauda epididymidis
Wade, M. A., Roman, S. D., Jones, R. C., & Aitken, R. J. (2003) · Cell and Tissue Research · doi:10.1007/s00441-003-0801-6 · Cited by 19 · see citing works
My Favourite Cell: The sea urchin spermatozoon
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Dephosphorylation of Sea Urchin Sperm Guanylate Cyclase During Fertilization
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Effect of some sex steroid hormones on human spermatozoa migration in vitro
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A novel group of sperm activating peptides from the sea urchin Glyptocidaris crenularis
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Non-genomic Effects of Steroid Hormones on Membrane Channels
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Effect of some sex steroid hormones on human spermatozoa migration in vitro
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To be or not to be (a receptor)
Wehling, M., Schultz, A., & Lösel, R. (2007) · Steroids · doi:10.1016/j.steroids.2006.11.007 · Cited by 9 · see citing works
Elgeti, J. (2006) · Kölner Universitäts PublikationsServer (Universität zu Köln) · Cited by 5 · see citing works
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Physics of microswimmers—single particle motion and collective behavior: a review
Elgeti, J., Winkler, R. G., & Gompper, G. (2015) · Reports on Progress in Physics · doi:10.1088/0034-4885/78/5/056601 · Cited by 1,472 · see citing works
“port of the genetic material to the egg. Here, chemotaxis is important to guide the spermotozoa to nd their target (Alvarez et al., 2014; Eisenbach and Giojalas, 2006; Friedrich and Julic her, 2007; Kaupp et al., 2008, 2003). Trypanosomes. Trypanosomes (T. brucei) are motile parasites responsible for sleeping sickness…”
BackgroundProgesterone activates the principal Ca2+ channel of human sperm
Lishko, P. V., Botchkina, I. L., & Kirichok, Y. (2011) · Nature · doi:10.1038/nature09767 · Cited by 617 · see citing works
Control of hyperactivation in sperm
Suárez, S. S. (2008) · Human Reproduction Update · doi:10.1093/humupd/dmn029 · Cited by 586 · see citing works
The CatSper channel mediates progesterone-induced Ca2+ influx in human sperm
Strünker, T., Goodwin, N., Brenker, C., Kashikar, N. D., Weyand, I., Seifert, R., & Kaupp, U. B. (2011) · Nature · doi:10.1038/nature09769 · Cited by 573 · see citing works
Gibbs, G. M., Roelants, K., & O’Bryan, M. K. (2008) · Endocrine Reviews · doi:10.1210/er.2008-0032 · Cited by 497 · see citing works
Rheotaxis Guides Mammalian Sperm
Miki, K., & Clapham, D. E. (2013) · Current Biology · doi:10.1016/j.cub.2013.02.007 · Cited by 445 · see citing works
“Regardless of fluid flow, these sea urchin sperm swam in circular planes (Movie S12B), consistent with the behavior of sperm from marine invertebrates that are not guided by marine currents but swim in concentric circles until encountering chemoattractants that turn and widen their arcs of circular motion [39].”
Acid Extrusion from Human Spermatozoa Is Mediated by Flagellar Voltage-Gated Proton Channel
Lishko, P. V., Botchkina, I. L., Fedorenko, A., & Kirichok, Y. (2010) · Cell · doi:10.1016/j.cell.2009.12.053 · Cited by 387 · see citing works
“Here, we report the successful application of the whole-cell patch-clamp technique to ejaculated human spermatozoa, which will allow studies of ion channels that control sperm motility, chemotaxis, and the acrosome reaction (Darszon et al., 2006; Kaupp et al., 2008; Navarro et al., 2008; Suarez, 2008) in completely ma…”
Calcium Channels in the Development, Maturation, and Function of Spermatozoa
Darszon, A., Nishigaki, T., Beltrán, C., & Treviño, C. L. (2011) · Physiological Reviews · doi:10.1152/physrev.00028.2010 · Cited by 362 · see citing works
“In spite of this, there is evidence suggesting that CaV3 channels may participate in sea urchin sperm chemotaxis (284, 563).”
BackgroundInfluentialRegulation of sperm storage and movement in the mammalian oviduct
Suárez, S. S. (2008) · The International Journal of Developmental Biology · doi:10.1387/ijdb.072527ss · Cited by 357 · see citing works
“%) are seen to respond chemotactically to physiological secretions, making the identification of active agents difficult (reviewed by M Eisenbach in this volume and Kaupp et al., 2007).”
BackgroundStructure, biology, evolution, and medical importance of sulfated fucans and galactans
Pomin, V. H., & Mourao, P. A. S. (2008) · Glycobiology · doi:10.1093/glycob/cwn085 · Cited by 356 · see citing works
The Control of Male Fertility by Spermatozoan Ion Channels
Lishko, P. V., Kirichok, Y., Ren, D., Navarro, B., Chung, J., & Clapham, D. E. (2011) · Annual Review of Physiology · doi:10.1146/annurev-physiol-020911-153258 · Cited by 341 · see citing works
“A more recent variation of this model proposes that cyclic nucleotides activate the hyperpolarization-activated and cyclic nucleotide– gated (HCN) channels, resulting in the depolarization and subsequent opening of Ca2+ channels (79, 152).”
BackgroundRheotaxis facilitates upstream navigation of mammalian sperm cells
Kantsler, V., Dunkel, J., Blayney, M. L., & Goldstein, R. E. (2014) · eLife · doi:10.7554/elife.02403 · Cited by 320 · see citing works
“Whilst chemotactic sensing (Kaupp et al., 2008) is assumed to provide important guidance in the immediate vicinity of the ovum (Spehr et al.”
Friedrich, B. M., & Jülicher, F. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0703530104 · Cited by 280 · see citing works
“There is evidence, that sperm cells can detect single chemoattractant molecules, when chemoattractant concentration is sub-pico-molar [78].”
BackgroundInfluentialSperm flagella: comparative and phylogenetic perspectives of protein components
Inaba, K. (2011) · Molecular Human Reproduction · doi:10.1093/molehr/gar034 · Cited by 287 · see citing works
“At fertilization, transient changes in intracellular Ca2+ concentration in response to the gradient of chemoattractant are necessary for momentary changes of flagellar wave asymmetry (Kaupp et al., 2008; Shiba et al., 2008; Guerrero et al., 2010).”
BackgroundFriedrich, B. M., Riedel-Kruse, I. H., Howard, J., & Jülicher, F. (2010) · Journal of Experimental Biology · doi:10.1242/jeb.039800 · Cited by 266 · see citing works
The CatSper channel: a polymodal chemosensor in human sperm
Brenker, C., Goodwin, N., Weyand, I., Kashikar, N. D., Naruse, M., Krähling, A. M., Müller, A., Kaupp, U. B., & Strünker, T. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.30 · Cited by 246 · see citing works
“…chemicals including vitamin D, chemokines, small peptides, the gas NO, neurotransmitters, analogues of cyclic nucleotides, and odorants (De Jonge, 2005; Eisenbach and Giojalas, 2006; Florman et al , 2008; Kaupp et al , 2008; Suarez, 2008) affect sperm motility and/or acrosomal exocytosis in vitro .”
Central role of soluble adenylyl cyclase and cAMP in sperm physiology
Buffone, M. G., Wertheimer, E. V., Visconti, P. E., & Krapf, D. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2014.07.013 · Cited by 213 · see citing works
“However, their role in sea urchin sperm chemotaxis is well known [142,143].”
BackgroundFlorman, H. M., & Fissore, R. A. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-397175-3.00004-1 · Cited by 199 · see citing works
The role of Hv1 and CatSper channels in sperm activation
Lishko, P. V., & Kirichok, Y. (2010) · The Journal of Physiology · doi:10.1113/jphysiol.2010.194142 · Cited by 197 · see citing works
“Journal compilation C© 2010 The Physiological Society (Kaupp et al. 2008).”
InfluentialIntracellular pH in sperm physiology
Nishigaki, T., José, O., González‐Cota, A. L., Romero, F., Treviño, C. L., & Darszon, A. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.05.100 · Cited by 175 · see citing works
Chen, S.-J., Allam, J., Duan, Y.-G., & Haidl, G. (2013) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-013-2801-4 · Cited by 163 · see citing works
Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone
Teves, M. E., Guidobaldi, H. A., Uñates, D. R., Sánchez, R., MISKA, W., Publicover, S. J., Morales-Garcia, A., & Giojalas, L. C. (2009) · PLoS ONE · doi:10.1371/journal.pone.0008211 · Cited by 158 · see citing works
“Calcium signaling plays a key role in sperm physiology [1,16,17], and it is well established that P at nM-mM concentrations can elevate [Ca]i in mammalian sperm.”
BackgroundResultInfluentialHenkel, R. (2011) · Asian Journal of Andrology · doi:10.1038/aja.2011.133 · Cited by 143 · see citing works
Oberholzer, M., Langousis, G., Nguyen, H. T., Saada, E. A., Shimogawa, M. M., Jónsson, Z. O., Nguyen, S. M., Wohlschlegel, J. A., & Hill, K. L. (2011) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m111.010538 · Cited by 137 · see citing works
“In other organisms, flagellum-dependent signaling is dominated by three signal transduction pathways: cyclic nucleotide signaling, Ca(2) signaling and phosphorylation cascades initiated by receptor-kinases on the flagellar membrane (2, 8).”
BackgroundAlvarez, L., Friedrich, B. M., Gompper, G., & Kaupp, U. B. (2013) · Trends in Cell Biology · doi:10.1016/j.tcb.2013.10.004 · Cited by 136 · see citing works
The insect spermatheca: an overview
Pascini, T. V., & Martins, G. F. (2016) · Zoology · doi:10.1016/j.zool.2016.12.001 · Cited by 135 · see citing works
Technology Roadmap of Micro/Nanorobots
Ju, X., Chen, C., Oral, Ç. M., Sevim, S., Golestanian, R., Sun, M., Bouzari, N., Lin, X., Urso, M., Nam, J. S., et al. (2025) · ACS Nano · doi:10.1021/acsnano.5c03911 · Cited by 139 · see citing works
Human sperm rheotaxis: a passive physical process
Zhang, Z., Liu, J., Meriano, J., Ru, C., Xie, S., Luo, J., & Sun, Y. (2016) · Scientific Reports · doi:10.1038/srep23553 · Cited by 134 · see citing works
Brunet, T., & Arendt, D. (2015) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2015.0043 · Cited by 129 · see citing works
Calcium Signaling Through CatSper Channels in Mammalian Fertilization
Ren, D., & Xia, J. (2010) · Physiology · doi:10.1152/physiol.00049.2009 · Cited by 126 · see citing works
Emergence of Upstream Swimming via a Hydrodynamic Transition
Tung, C.-K., Ardón, F., Roy, A., Koch, D. L., Suárez, S. S., & Wu, M. (2015) · Physical Review Letters · doi:10.1103/physrevlett.114.108102 · Cited by 124 · see citing works
The Ca2+-activated K+ current of human sperm is mediated by Slo3
Brenker, C., Zhou, Y., Müller, A., Echeverry, F. A., Trötschel, C., Poetsch, A., Xia, X., Bönigk, W., Lingle, C. J., Kaupp, U. B., et al. (2014) · eLife · doi:10.7554/elife.01438 · Cited by 122 · see citing works
“Navigation, capacitation, hyperactivation, and acrosomal exocytosis are controlled by changes in intracellular pH (pHi), membrane voltage (Vm), and intracellular Ca2+ concentration ([Ca]i) (Ho and Suarez, 2001; Eisenbach and Giojalas, 2006; Florman et al., 2008; Kaupp et al., 2008; Publicover et al., 2008).”
The Trypanosoma brucei Flagellum: Moving Parasites in New Directions
Ralston, K. S., Kabututu, Z. P., Melehani, J. H., Oberholzer, M., & Hill, K. L. (2009) · Annual Review of Microbiology · doi:10.1146/annurev.micro.091208.073353 · Cited by 119 · see citing works
“In mammalian sperm, flagellar cyclic nucleotide and calcium signaling are crucial for chemotaxis and maturation (32, 38, 61).”
BackgroundSperm Trajectories Form Chiral Ribbons
Su, T., Choi, I., Feng, J., Huang, K., McLeod, E. J., & Özcan, A. (2013) · Scientific Reports · doi:10.1038/srep01664 · Cited by 116 · see citing works
Chemotactic Guidance of Synthetic Organic/Inorganic Payloads Functionalized Sperm Micromotors
Chen, C., Chang, X., Angsantikul, P., Li, J., de Ávila, B. E., Karshalev, E., Liu, W., Mou, F., He, S., Castillo, R., et al. (2017) · Advanced Biosystems · doi:10.1002/adbi.201700160 · Cited by 115 · see citing works
Pomin, V. H. (2009) · Biopolymers · doi:10.1002/bip.21200 · Cited by 115 · see citing works
Origin and early evolution of neural circuits for the control of ciliary locomotion
Jékely, G. (2011) · Proceedings of the Royal Society B: Biological Sciences · doi:10.1098/rspb.2010.2027 · Cited by 103 · see citing works
Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility
Darszon, A., Guerrero, A., Galindo, B. E., Nishigaki, T., & Wood, C. D. (2008) · The International Journal of Developmental Biology · doi:10.1387/ijdb.072550ad · Cited by 111 · see citing works
Bimodal rheotactic behavior reflects flagellar beat asymmetry in human sperm cells
Bukatin, A., Kukhtevich, I. V., Stoop, N., Dunkel, J., & Kantsler, V. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1515159112 · Cited by 110 · see citing works
“Earlier studies of human sperm locomotion have identified several potential steering and transport mechanisms, including thermotaxis (4), uterine peristalsis (29, 30), and chemotaxis (14, 16, 31), but their relative importance has yet to be quantified.”
BackgroundTuning sperm chemotaxis by calcium burst timing
Guerrero, A., Nishigaki, T., Carneiro, J., Tatsu, Y., Wood, C. D., & Darszon, A. (2010) · Developmental Biology · doi:10.1016/j.ydbio.2010.04.013 · Cited by 108 · see citing works
Rediscovering sperm ion channels with the patch-clamp technique
Kirichok, Y., & Lishko, P. V. (2011) · Molecular Human Reproduction · doi:10.1093/molehr/gar044 · Cited by 110 · see citing works
“Each turn is caused by a brief asymmetrical tail motion that is triggered by a transient elevation of Ca2+ in the sperm flagellum (Kaupp et al., 2008; Guerrero et al., 2010).”
BackgroundThe rate of change in Ca2+ concentration controls sperm chemotaxis
Alvarez, L., Dai, L., Friedrich, B. M., Kashikar, N. D., Gregor, I., Pascal, R., & Kaupp, U. B. (2012) · The Journal of Cell Biology · doi:10.1083/jcb.201106096 · Cited by 107 · see citing works
“The binding of chemoattractant molecules to surface receptors initiates a series of signaling events that produce Ca2+ signals in the flagellum (Matsumoto et al., 2003; Wood et al., 2007; Kaupp et al., 2008; Shiba et al., 2008).”
Salek, M. M., Carrara, F., Fernandez, V. I., Guasto, J., & Stocker, R. (2019) · Nature Communications · doi:10.1038/s41467-019-09521-2 · Cited by 101 · see citing works
Mechanical Signaling in Networks of Motor and Cytoskeletal Proteins
Howard, J. (2009) · Annual Review of Biophysics · doi:10.1146/annurev.biophys.050708.133732 · Cited by 100 · see citing works
“Sea urchin sperm chemotax: They target eggs in the open water by swimming up a concentration gradient of a small peptide that they detect through a sensitive guanyly-cyclase receptor pathway (34).”
BackgroundSwimming with magnets: From biological organisms to synthetic devices
Klumpp, S., Lefèvre, C. T., Bennet, M. A., & Faivre, D. (2018) · Physics Reports · doi:10.1016/j.physrep.2018.10.007 · Cited by 100 · see citing works
Beirão, J., Zilli, L., Vilella, S., Cabrita, E., Schiavone, R., & Herráez, M. P. (2011) · Biology of Reproduction · doi:10.1095/biolreprod.111.093401 · Cited by 99 · see citing works
“Changes in motility pattern have been related to different factors: cryopreservation protocols [26], effect of stimulants [40], time after release to water in fish [41], chemotaxis [42], or the hyperactivation subsequent to the capacitation process in mammals [43, 44].”
BackgroundTung, C.-K., Ardón, F., Fiore, A. G., Suárez, S. S., & Wu, M. (2014) · Lab on a Chip · doi:10.1039/c3lc51297e · Cited by 98 · see citing works
The stochastic dance of circling sperm cells: sperm chemotaxis in the plane
Friedrich, B. M., & Jülicher, F. (2008) · New Journal of Physics · doi:10.1088/1367-2630/10/12/123025 · Cited by 97 · see citing works
“A chemoattractant receptor becomes activated upon binding a chemoattractant molecule and deactivates again on a timescale of 300ms [7].”
BackgroundInfluentialRethinking the Relationship Between Hyperactivation and Chemotaxis in Mammalian Sperm1
Chang, H., & Suárez, S. S. (2010) · Biology of Reproduction · doi:10.1095/biolreprod.109.083113 · Cited by 97 · see citing works
“[2]).”
The CatSper channel controls chemosensation in sea urchin sperm
Seifert, R., Flick, M., Bönigk, W., Alvarez, L., Trötschel, C., Poetsch, A., Müller, A., Goodwin, N., Pelzer, P., Kashikar, N. D., et al. (2014) · The EMBO Journal · doi:10.15252/embj.201489376 · Cited by 96 · see citing works
“Important components and cellular events of this signaling pathway have been identified (reviewed in Darszon et al, 2008; Kaupp et al, 2008; Alvarez et al, 2014).”
BackgroundHolt, W. V., & Fazeli, A. (2015) · Molecular Human Reproduction · doi:10.1093/molehr/gav012 · Cited by 92 · see citing works
“, 2011), and the consequent modulation of intracellular calcium concentrations that change flagellar activity (Kaupp et al., 2008).”
BackgroundSteering Chiral Swimmers along Noisy Helical Paths
Friedrich, B. M., & Jülicher, F. (2009) · Physical Review Letters · doi:10.1103/physrevlett.103.068102 · Cited by 91 · see citing works
Fluoride-induced apoptosis and gene expression profiling in mice sperm in vivo
Sun, Z., Niu, R., Wang, B., Jiao, Z., Wang, J., Zhang, J., Wang, S., & Wang, J. (2011) · Archives of Toxicology · doi:10.1007/s00204-011-0672-7 · Cited by 85 · see citing works
“Interestingly, signal transduction is involved to diverse sperm biological function as capacitation, acrosome reaction, hyperactiviation, chemotaxis, and sperm– egg fusion (Eisenbach and Giojalas 2006; Kaupp et al. 2008; Suarez 2008).”
BackgroundSperm chemotaxis, fluid shear, and the evolution of sexual reproduction
Zimmer, R. K., & Riffell, J. A. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1018666108 · Cited by 84 · see citing works
Two Distinct Ca2+ Signaling Pathways Modulate Sperm Flagellar Beating Patterns in Mice1
Chang, H., & Suárez, S. S. (2011) · Biology of Reproduction · doi:10.1095/biolreprod.110.089789 · Cited by 84 · see citing works
“This alternation forms a ‘‘turn and run’’ pattern, which produces a loosely helical configuration with an axis that is directed toward the source of the attractant (for review, see [68]).”
BackgroundSpeciation Genes in Free-Spawning Marine Invertebrates
Lessios, H. A. (2011) · Integrative and Comparative Biology · doi:10.1093/icb/icr039 · Cited by 83 · see citing works
“Many molecules on gamete surfaces are known to mediate these steps and many more remain to be discovered (reviews by Ward and Kopf 1993; Vacquier 1998; Neill and Vacquier 2004; Kaupp et al. 2006; Vieira and Miller 2006; Hirohashi et al. 2008; Kaupp et al. 2008).”
BackgroundThe computational worm: spatial orientation and its neuronal basis in C. elegans
Lockery, S. R. (2011) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2011.06.009 · Cited by 78 · see citing works
Meyer, D., Voigt, A., Widmayer, P., Borth, H., Huebner, S., Breit, A., Marschall, S., de Angelis, M. H., Boehm, U., Meyerhof, W., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0032354 · Cited by 77 · see citing works
“surrounding structures (chemotaxis) (for review see [10,11,12]).”
Chemosensory Ca2+ Dynamics Correlate with Diverse Behavioral Phenotypes in Human Sperm
Veitinger, T., Riffell, J. R., Veitinger, S., Nascimento, J. M., Triller, A., Chandsawangbhuwana, C., Schwane, K., Geerts, A., Wunder, F., Berns, M. W., et al. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.211524 · Cited by 79 · see citing works
An Atypical CNG Channel Activated by a Single cGMP Molecule Controls Sperm Chemotaxis
Bönigk, W., Loogen, A., Seifert, R., Kashikar, N. D., Klemm, C., Krause, E., Hagen, V., Kremmer, E., Strünker, T., & Kaupp, U. B. (2009) · Science Signaling · doi:10.1126/scisignal.2000516 · Cited by 78 · see citing works
“Ca entry is controlled by a guanosine 3′,5′-monophosphate (cGMP)–signaling pathway that involves a chemoattractant receptor and various ion channels (6).”
BackgroundEnergetics of fish spermatozoa: The proven and the possible
Dzyuba, B., Bondarenko, O., Fedorov, P. S., Gazo, I., Prokopchuk, G., & Cosson, J. (2016) · Aquaculture · doi:10.1016/j.aquaculture.2016.05.038 · Cited by 78 · see citing works
Hoang, H. D., PRASAIN, J. K., Dorand, R. D., & Miller, M. A. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003271 · Cited by 72 · see citing works
“While much has been learned about sperm motility in aquatic species [5,6], the mechanisms that guide sperm to oocytes in internally fertilizing animals are less well understood.”
BackgroundSimulating nature in sperm selection for assisted reproduction
Leung, E. T. Y., Lee, C., Tian, X., Lam, K. K. W., Li, R., Ng, E. H. Y., Yeung, W. S. B., & Chiu, P. C. N. (2021) · Nature Reviews Urology · doi:10.1038/s41585-021-00530-9 · Cited by 75 · see citing works
Sperm form and function in the absence of sperm competition
van der Horst, G., & Maree, L. (2013) · Molecular Reproduction and Development · doi:10.1002/mrd.22277 · Cited by 75 · see citing works
“Furthermore, measures of fitness or sperm function should be identified and assessed, such as the ability to initiate Ca2þ signaling pathways for changes in sperm flagellar output associated with chemotaxis (Darszon et al., 2006; Kaupp et al., 2008) or sperm hyperactivation (Suarez and Ho, 2003; Suarez, 2008).”
BackgroundFactors controlling sperm migration through the oviduct revealed by gene-modified mouse models
Fujihara, Y., Miyata, H., & Ikawa, M. (2018) · EXPERIMENTAL ANIMALS · doi:10.1538/expanim.17-0153 · Cited by 74 · see citing works
“COC factors that modulate sperm functions Sperm chemotaxis is found in not only marine invertebrates but also mammals [44].”
BackgroundKholodnyy, V., Gadêlha, H., Cosson, J., & Boryshpolets, S. (2019) · Reviews in Aquaculture · doi:10.1111/raq.12378 · Cited by 73 · see citing works
“For example, spermatozoa of the sea urchin A. punctulata will swim in to circles until sensing the resact molecule, which will result in rapid, specific ‘turn-and-run’ trajectories to approach the source of the attractant molecules (Kaupp et al. 2008).”
BackgroundInfluentialSaada, E. A., Kabututu, Z. P., López, M., Shimogawa, M. M., Langousis, G., Oberholzer, M., Riestra, A. M., Jónsson, Z. O., Wohlschlegel, J. A., & Hill, K. L. (2014) · Eukaryotic Cell · doi:10.1128/ec.00019-14 · Cited by 69 · see citing works
Chemotactic predator-prey dynamics
Sengupta, A., Kruppa, T., & Löwen, H. (2011) · Physical Review E · doi:10.1103/physreve.83.031914 · Cited by 72 · see citing works
Cooperative and uncooperative cyclic-nucleotide-gated ion channels
Cukkemane, A., Seifert, R., & Kaupp, U. B. (2010) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2010.07.004 · Cited by 71 · see citing works
Advances in sperm analysis: techniques, discoveries and applications
Dai, C., Zhang, Z., Shan, G., Chu, L.-T., Huang, Z., Moskovtsev, S. I., Librach, C. L., Jarvi, K. A., & Sun, Y. (2021) · Nature Reviews Urology · doi:10.1038/s41585-021-00472-2 · Cited by 70 · see citing works
CatSper: The complex main gate of calcium entry in mammalian spermatozoa
Rahban, R., & Nef, S. (2020) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2020.110951 · Cited by 69 · see citing works
Potassium Channels Keep Mobile Cells on the Go
Schwab, A., Hanley, P. J., Fabian, A., & Stock, C. M. (2008) · Physiology · doi:10.1152/physiol.00003.2008 · Cited by 65 · see citing works
Applying Movement Ecology to Marine Animals with Complex Life Cycles
Allen, R. M., Meta×as, A., & Snelgrove, P. V. R. (2017) · Annual Review of Marine Science · doi:10.1146/annurev-marine-121916-063134 · Cited by 65 · see citing works
Smith, C. L., Reese, T. S., Govezensky, T., & Barrio, R. A. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1815655116 · Cited by 61 · see citing works
“Some larger cells, such as sperm, are able to adjust their helical paths so as to steer more persistently up the gradient (37, 38, 40, 41).”
BackgroundOptimal Prediction by Cellular Signaling Networks
Becker, N. B., Mugler, A., & ten Wolde, P. R. (2015) · Physical Review Letters · doi:10.1103/physrevlett.115.258103 · Cited by 63 · see citing works
Levin, R. J. (2011) · The Journal of Sexual Medicine · doi:10.1111/j.1743-6109.2010.02162.x · Cited by 62 · see citing works
Independent Control of the Static and Dynamic Components of the Chlamydomonas Flagellar Beat
Geyer, V. F., Sartori, P., Friedrich, B. M., Jülicher, F., & Howard, J. (2016) · Current Biology · doi:10.1016/j.cub.2016.02.053 · Cited by 62 · see citing works
Characterization of rheotaxis of bull sperm using microfluidics
El-Sherry, T. M., Elsayed, M., Hafez, H. K. A., & Abdelgawad, M. O. (2014) · Integrative Biology · doi:10.1039/c4ib00196f · Cited by 61 · see citing works
Flagellar cAMP signaling controls trypanosome progression through host tissues
Shaw, S., DeMarco, S. F., Rehmann, R., Wenzler, T., Florini, F., Roditi, I. J., & Hill, K. L. (2019) · Nature Communications · doi:10.1038/s41467-019-08696-y · Cited by 59 · see citing works
A K+-selective CNG channel orchestrates Ca2+ signalling in zebrafish sperm
Fechner, S., Alvarez, L., Bönigk, W., Müller, A., Berger, T. K., Pascal, R., Trötschel, C., Poetsch, A., Stölting, G., Siegfried, K. R., et al. (2015) · eLife · doi:10.7554/elife.07624 · Cited by 57 · see citing works
“These sensory cues activate various cellular signalling pathways that ultimately control the intracellular Ca concentration ([Ca]i) and, thereby, the flagellar beat and swimming behaviours (Alvarez et al., 2012; Darszon et al., 2008; Eisenbach and Giojalas, 2006; Florman et al., 2008; Guerrero et al., 2010; Ho and Sua…”
BackgroundGuerrero, A., Carneiro, J., Pimentel, J. A., Wood, C. D., Corkidi, G., & Darszon, A. (2011) · Molecular Human Reproduction · doi:10.1093/molehr/gar042 · Cited by 54 · see citing works
“In this section, we review current knowledge of sperm chemotaxis of marine spermatozoa in 2D [((Darszon et al., 2008; Kaupp et al., 2008; Guerrero et al., 2010b); mammalian sperm chemotaxis is reviewed in (Publicover et al., 2007; Kaupp et al., 2008; Chang and Suarez, 2010))].”
BackgroundMethodInfluentialPolyspermy prevention: facts and artifacts?
Dale, B., & DeFelice, L. J. (2010) · Journal of Assisted Reproduction and Genetics · doi:10.1007/s10815-010-9513-5 · Cited by 55 · see citing works
“To ensure gamete encounter, spermatozoa are attracted towards the oocyte or gonangial extract by specific chemo-attractants [16, 61 , 71, 75].”
Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa
Berendsen, J. T. W., Kruit, S. A., Atak, N., Willink, E., & Segerink, L. I. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.9b05183 · Cited by 55 · see citing works
Regulation of flagellar motility by the conserved flagellar protein CG34110/Ccdc135/FAP50
Yang, Y. G., Cochran, D. A., Gargano, M. D., King, I., Samhat, N. K., Burger, B. P., Sabourin, K. R., Hou, Y., Awata, J., Parry, D. A. D., et al. (2011) · Molecular Biology of the Cell · doi:10.1091/mbc.e10-04-0331 · Cited by 54 · see citing works
“A well-known mechanism for eliciting motility changes is alteration of intraflagellar Ca2+ concentrations (Kaupp et al., 2008; King, 2010).”
BackgroundZhang, Y., Xiao, R., Yin, T., Zou, W., Tang, Y., Ding, J., & Yang, J. (2015) · PLoS ONE · doi:10.1371/journal.pone.0142555 · Cited by 53 · see citing works
“The population of sperm which can fertilize the egg is even smaller [1, 2].”
BackgroundSeparation of Progressive Motile Sperm from Mouse Semen Using On-chip Chemotaxis
Ko, Y., Maeng, J., Lee, B. C., Lee, S., Hwang, S. Y., & Ahn, Y. (2012) · Analytical Sciences · doi:10.2116/analsci.28.27 · Cited by 51 · see citing works
Dynamics of a microorganism moving by chemotaxis in its own secretion
Sengupta, A., van Teeffelen, S., & Löwen, H. (2009) · Physical Review E · doi:10.1103/physreve.80.031122 · Cited by 50 · see citing works
Sperm motility in modulated microchannels
Rode, S., Elgeti, J., & Gompper, G. (2018) · New Journal of Physics · doi:10.1088/1367-2630/aaf544 · Cited by 50 · see citing works
Krähling, A. M., Alvarez, L., Debowski, K., Van, Q., Gunkel, M., Irsen, S. H., Al‐Amoudi, A., Strünker, T., Kremmer, E., Krause, E., et al. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003960 · Cited by 49 · see citing works
Melatonin in Sperm Biology: Breaking Paradigms
Cebrián-Pérez, J. Á., Casao, A., González-Arto, M., Hamilton, T. D. S., Pérez‐Pé, R., & Muiño-Blanco, T. (2014) · Reproduction in Domestic Animals · doi:10.1111/rda.12378 · Cited by 48 · see citing works
“…mechanism by which sperm are guided by a chemical gradient to reach the oocyte has been well documented in marine invertebrates with external fertilization (Reviewed by (Kaupp et al. 2008)) and, more recently, has been evidenced in mammalian sperm (reviewed in (Eisenbach and Giojalas 2006)).”
BackgroundDrosophila Sperm Motility in the Reproductive Tract1
Yang, Y. G., & Lu, X. (2011) · Biology of Reproduction · doi:10.1095/biolreprod.110.088773 · Cited by 48 · see citing works
“A general phenomenon related to sperm in chemotactic settings is that only a small fraction (usually ,10%) respond chemotactically to physiological secretions (for review, see [1, 19]).”
BackgroundFemale gametophyte-controlled pollen tube guidance
Márton, M. L., & Dresselhaus, T. (2010) · Biochemical Society Transactions · doi:10.1042/bst0380627 · Cited by 47 · see citing works
Konno, A., Setou, M., & Ikegami, K. (2012) · International review of cytology · doi:10.1016/b978-0-12-394305-7.00003-3 · Cited by 47 · see citing works
K+ and Cl− Channels and Transporters in Sperm Function
Santi, C. M., Orta, G., Salkoff, L., Visconti, P. E., Darszon, A., & Treviño, C. L. (2012) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/b978-0-12-416024-8.00014-3 · Cited by 42 · see citing works
Chang, H., Kim, B. J., Kim, Y. S., Suárez, S. S., & Wu, M. (2013) · PLoS ONE · doi:10.1371/journal.pone.0060587 · Cited by 41 · see citing works
“Sperm chemotaxis, where sperm are guided toward eggs by their secretions, has been demonstrated to occur in some species [5,6]; however, it is not clear if chemotaxis is a universal strategy for sperm of all species to find eggs.”
BackgroundUpward swimming of a sperm cell in shear flow
Omori, T., & Ishikawa, T. (2016) · Physical review. E · doi:10.1103/physreve.93.032402 · Cited by 41 · see citing works
A comparison of early molecular fertilization mechanisms in animals and flowering plants
Márton, M. L., & Dresselhaus, T. (2008) · Sexual Plant Reproduction · doi:10.1007/s00497-007-0062-8 · Cited by 41 · see citing works
“In turn, this affects flagellar motion and finally determines the direction of movement (Ramarao and Garbers 1985; Bentley et al. 1986; Nishigaki et al. 2000; Matsumoto et al. 2003; Neill and Vacquier 2004; Kaupp et al. 2008).”
BackgroundInfluentialTemporal sampling, resetting, and adaptation orchestrate gradient sensing in sperm
Kashikar, N. D., Alvarez, L., Seifert, R., Gregor, I., Jäckle, O., Beyermann, M., Krause, E., & Kaupp, U. B. (2012) · The Journal of Cell Biology · doi:10.1083/jcb.201204024 · Cited by 41 · see citing works
“Sperm are attracted to the egg by chemical substances, a process called chemotaxis (Eisenbach and Giojalas, 2006; Kaupp et al., 2008).”
Human sperm pattern of movement during chemotactic re-orientation towards a progesterone source
Blengini, C. S., Teves, M. E., Uñates, D. R., Guidobaldi, H. A., Gatica, L. V., & Giojalas, L. C. (2011) · Asian Journal of Andrology · doi:10.1038/aja.2011.27 · Cited by 38 · see citing works
Laboratory on sea urchin fertilization
Vacquier, V. D. (2011) · Molecular Reproduction and Development · doi:10.1002/mrd.21360 · Cited by 38 · see citing works
“A single sperm cell can sense one molecule of resact bound to its flagellum (Kaupp et al., 2008).”
Background
- Authors
- Kaupp, U. B., Kashikar, N. D., & Weyand, I.
- Affiliations
- Forschungszentrum Jülich (DE)
- Venue
- Annual Review of Physiology
- Publisher
- Annual Reviews
- Published
- 2008-03-01
- Volume
- 70
- Issue
- 1
- Pages
- 93-117
- ISSN
- 0066-4278 (ISSN-L)
- 1545-1585
- OpenAlex
- W2155214806
- Cited by
- 284
- References
- 144
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 12 · 2023: 7 · 2022: 11 · 2021: 18 · 2020: 18 · 2019: 14 · 2018: 8 · 2017: 19 · 2016: 10 · 2015: 19 · 2014: 23 · 2013: 23 · 2012: 14
- FWCI
- 2.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Annual Review of Physiology (publishedVersion)
- PubMed (publishedVersion)
- JuSER (Forschungszentrum Jülich) (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Keywords
- sperm chemotaxis, chemotactic signaling, chemoattractants, sea urchin sperm, mammalian sperm, sensory signaling, photoreceptors, olfactory sensory neurons
- MeSH terms
- Animals, Chemotaxis, Humans, Male, Sperm Motility, Spermatozoa, Signal Transduction
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