The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Interspecies communication in Streptococcus gordonii–Veillonella atypica biofilms: Signaling in flow conditions requires juxtaposition
Open accessAbstract
During the development of human oral biofilm communities, the spatial arrangement of the bacteria is thought to be driven by metabolic interactions between them. Streptococcus gordonii and Veillonella atypica , two early colonizing members of the dental plaque biofilm, have been postulated to participate in metabolic communication; S. gordonii ferments carbohydrates to form lactic acid, which is a preferred fermentation substrate for V. atypica . We found that, during agar-plate coculture of these organisms, a signaling event occurs that results in increased expression of the S. gordonii α-amylase-encoding gene amyB . Confocal scanning laser microscopy of coculture flowcell-grown biofilms using human saliva as the sole nutrient showed that V. atypica caused S. gordonii to increase expression of a PamyB -′ gfp transcriptional fusion in a spatially resolved fashion. In this open system, only those streptococci in mixed-species microcolonies containing V. atypica expressed GFP; nearby S. gordonii colonies that lacked V. atypica did not express GFP. In a closed system containing S. gordonii and V. atypica , flow cytometric analysis showed that S. gordonii containing the PamyB -′ gfp reporter plasmid exhibited mean fluorescence levels 20-fold higher than did S. gordonii that had not been incubated with V. atypica . Thus, in a closed system where a diffusible signal can accumulate above a required threshold, interspecies signaling mediates a change in gene expression. We provide evidence that, in open systems like those that predominate in natural biofilms, diffusible signals between species are designed to function over short distances, on the order of 1 μm.
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Communication among Oral Bacteria
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Self-citationCross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi
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Is quorum sensing a side effect of diffusion sensing?
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Self-citationGrowth of several cariogenic strains of oral streptococci in a chemically defined medium
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Cited by
Oral multispecies biofilm development and the key role of cell–cell distance
Kolenbrander, P. E., Palmer, R. J., Periasamy, S., & Jakubovics, N. S. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2381 · Cited by 1,309
Self-citationBacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults
Aas, J. A., Griffen, A. L., Dardis, S. R., Lee, A. M., Olsen, I., Dewhirst, F. E., Leys, E. J., & Paster, B. J. (2008) · Journal of Clinical Microbiology · doi:10.1128/jcm.01410-07 · Cited by 952
Bacterial interactions and successions during plaque development
Kolenbrander, P. E., Palmer, R. J., Rickard, A. H., Jakubovics, N. S., Chalmers, N. I., & Diaz, P. I. (2006) · Periodontology 2000 · doi:10.1111/j.1600-0757.2006.00187.x · Cited by 791
Self-citationStreptococcus Adherence and Colonization
Nobbs, A. H., Lamont, R. J., & Jenkinson, H. F. (2009) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00014-09 · Cited by 657
Interspecies Interactions within Oral Microbial Communities
Kuramitsu, H. K., He, X., Lux, R., Anderson, M. H., & Shi, W. (2007) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00024-07 · Cited by 600
Metadata
- Authors
- Egland, P. G., Palmer, R. J., & Kolenbrander, P. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-11-16
- Volume
- 101
- Issue
- 48
- Pages
- 16917-16922
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2091337262
- Cited by
- 256
- References
- 24
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- green
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