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Fragile X mental retardation protein is necessary for neurotransmitter-activated protein translation at synapses
Open accessAbstract
Fragile X mental retardation is caused by absence of the RNA-binding protein fragile X mental retardation protein (FMRP), encoded by the FMR1 gene. There is increasing evidence that FMRP regulates transport and modulates translation of some mRNAs. We studied neurotransmitter-activated synaptic protein synthesis in fmr1 -knockout mice. Synaptoneurosomes from knockout mice did not manifest accelerated polyribosome assembly or protein synthesis as it occurs in wild-type mice upon stimulation of group I metabotropic glutamate receptors. Direct activation of protein kinase C did not compensate in the knockout mouse, indicating that the FMRP-dependent step is further along the signaling pathway. Visual cortices of young knockout mice exhibited a lower proportion of dendritic spine synapses containing polyribosomes than did the cortices of wild-type mice, corroborating this finding in vivo . This deficit in rapid neurotransmitter-controlled local translation of specific proteins may contribute to morphological and functional abnormalities observed in patients with fragile X syndrome.
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“However, we did not observe an increase in overall protein synthesis in response to DHPG, whereas Weiler and colleagues [31] observed a 1.3-fold increase in 35S-Met incorporation after 5 min of stimulation.”
From FMRP Mediates mGluR5-Dependent Translation of Amyloid Precursor Protein (2007) · cites this paper as [31]
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“Therefore, FMRP was not required for basal protein synthesis, which is in agreement with a prior report [31].”
From FMRP Mediates mGluR5-Dependent Translation of Amyloid Precursor Protein (2007) · cites this paper as [31]
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Cites
The unbiased estimation of number and sizes of arbitrary particles using the disector
Sterio, D. C. (1984) · Journal of Microscopy · doi:10.1111/j.1365-2818.1984.tb02501.x · Cited by 2,533
Gundersen, H. J. G., Bagger, P. V., Bendtsen, T. F., Evans, S. M., Korbo, L., Marcussen, N., Møller, A., Nielsen, K. N., Nyengaard, J. R., Pakkenberg, B., et al. (1988) · Apmis · doi:10.1111/j.1699-0463.1988.tb00954.x · Cited by 2,480
Altered synaptic plasticity in a mouse model of fragile X mental retardation
Huber, K. M., Gallagher, S. M., Warren, S. T., & Bear, M. F. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.122205699 · Cited by 1,349
Brown, V., Jin, P., Ceman, S., Darnell, J. C., O'Donnell, W. T., Tenenbaum, S. A., Jin, X., Feng, Y., Wilkinson, K. D., Keene, J. D., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00568-2 · Cited by 1,109
Fmr1 knockout mice: A model to study fragile X mental retardation
THEDUTCHBELGIANFRAGILEXCONSOR (1994) · Cell · doi:10.1016/0092-8674(94)90569-x · Cited by 1,108
Abnormal dendritic spines in fragile X knockout mice: Maturation and pruning deficits
Comery, T. A., Harris, J. B., Willems, P. J., Oostra, B. A., Irwin, S. A., Weiler, I. J., & Greenough, W. T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.10.5401 · Cited by 1,046
Self-citationFragile X mental retardation protein targets G quartet mRNAs important for neuronal function
Darnell, J. C., Jensen, K. B., Jin, P., Brown, V., Warren, S. T., & Darnell, R. B. (2001) · Cell · doi:10.1016/s0092-8674(01)00566-9 · Cited by 952
Devys, D., Lutz, Y., Rouyer, N., Bellocq, J., & Mandel, J. (1993) · Nature Genetics · doi:10.1038/ng0893-335 · Cited by 781
Protein synthesis at synaptic sites on dendrites.
Steward, O., & Schuman, E. (2001) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.24.1.299 · Cited by 731
Weiler, I. J., Irwin, S. A., Klintsova, A. Y., Spencer, C. M., Brazelton, A. D., Miyashiro, K., Comery, T. A., Patel, B., Eberwine, J., & Greenough, W. T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.10.5395 · Cited by 644
Self-citationThe protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein.
Siomi, H., Siomi, M. C., Nussbaum, R. L., & Dreyfuss, G. (1993) · Cell · doi:10.1016/0092-8674(93)90420-u · Cited by 641
A point mutation in the FMR-1 gene associated with fragile X mental retardation
Boulle, K. D., Verkerk, A. J. M. H., Reyniers, E., Vits, L., Hendrickx, J., Roy, B. V., Bos, F. V. D., de Graaff, E., Oostra, B. A., & Willems, P. J. (1993) · Nature Genetics · doi:10.1038/ng0193-31 · Cited by 624
Evidence that fragile X mental retardation protein is a negative regulator of translation
Laggerbauer, B. (2001) · Human Molecular Genetics · doi:10.1093/hmg/10.4.329 · Cited by 571
Krichevsky, A. M., & Kosik, K. S. (2001) · Neuron · doi:10.1016/s0896-6273(01)00508-6 · Cited by 536
Feng, Y., Gutekunst, C.-A., Eberhart, D. E., Yi, H., Warren, S. T., & Hersch, S. M. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-05-01539.1997 · Cited by 532
Analysis of neocortex in three males with the Fragile X syndrome
Hinton, V. J., Brown, W. T., Wisniewski, K., & Rudelli, R. D. (1991) · American Journal of Medical Genetics · doi:10.1002/ajmg.1320410306 · Cited by 527
RNA cargoes associating with FMRP reveal deficits in cellular functioning in Fmr1 null mice
Miyashiro, K. Y., Beckel-Mitchener, A., Purk, T. P., Becker, K. G., Barret, T., Liu, L., Carbonetto, S., Weiler, I. J., Greenough, W. T., & Eberwine, J. (2003) · Neuron · doi:10.1016/s0896-6273(03)00034-5 · Cited by 495
Self-citationFragile X Syndrome: Diagnosis, Treatment, And Research
Silverman, & Cronister, A. (1991) · Johns Hopkins University Press eBooks · Cited by 481
The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif
Schaeffer, C., Bardoni, B., Mandel, J., Ehresmann, B., Ehresmann, C., & Moine, H. (2001) · The EMBO Journal · doi:10.1093/emboj/20.17.4803 · Cited by 471
Ostroff, L. E., Fiala, J. C., Allwardt, B., & Harris, K. M. (2002) · Neuron · doi:10.1016/s0896-6273(02)00785-7 · Cited by 461
Pharmacological and functional characteristics of metabotropic excitatory amino acid receptors
Schoepp, D. D., Bockaert, J., & Sladeczek, F. A. J. (1990) · Trends in Pharmacological Sciences · doi:10.1016/0165-6147(90)90052-a · Cited by 458
The fragile X mental retardation protein inhibits translation via interacting with mRNA
Li, Z. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.11.2276 · Cited by 448
Antar, L. N., Afroz, R., Dictenberg, J. B., Carroll, R. C., & Bassell, G. J. (2004) · The Journal of Neuroscience · doi:10.1523/jneurosci.0099-04.2004 · Cited by 411
Hollingsworth, E. B., McNeal, E., Burton, J. L., Williams, R., Daly, J. W., & Creveling, C. R. (1985) · Journal of Neuroscience · doi:10.1523/jneurosci.05-08-02240.1985 · Cited by 408
Mazrouï, R. (2002) · Human Molecular Genetics · doi:10.1093/hmg/11.24.3007 · Cited by 369
Phosphorylation influences the translation state of FMRP-associated polyribosomes
Ceman, S., O'Donnell, W. T., Reed, M., Patton, S., Pohl, J., & Warren, S. T. (2003) · Human Molecular Genetics · doi:10.1093/hmg/ddg350 · Cited by 327
Tiruchinapalli, D. M., Oleynikov, Y., Kelič, S., Shenoy, S. M., Hartley, A., Stanton, P. K., Singer, R. H., & Bassell, G. J. (2003) · Journal of Neuroscience · doi:10.1523/jneurosci.23-08-03251.2003 · Cited by 320
Synaptic regulation of protein synthesis and the fragile X protein
Greenough, W. T., Klintsova, A. Y., Irwin, S. A., Galvez, R., Bates, K. E., & Weiler, I. J. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.141145998 · Cited by 317
Self-citationThe brain-derived neurotrophic factor enhances synthesis of Arc in synaptoneurosomes
Yin, Y., Edelman, G. M., & Vanderklish, P. W. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.042693699 · Cited by 286
Fragile X mental retardation protein is associated with translating polyribosomes in neuronal cells
Stefani, G., Fraser, C. E., Darnell, J. C., & Darnell, R. B. (2004) · The Journal of Neuroscience · doi:10.1523/jneurosci.2306-04.2004 · Cited by 274
Irwin, S. A., Idupulapati, M., Gilbert, M. E., Harris, J. B., Chakravarti, A. B., Rogers, E. J., Crisostomo, R. A., Larsen, B. P., Mehta, A., Alcantara, C. J., et al. (2002) · American Journal of Medical Genetics · doi:10.1002/ajmg.10500 · Cited by 270
Self-citationMetabotropic glutamate receptors trigger postsynaptic protein synthesis.
Weiler, I., & Greenough, W. (1993) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.90.15.7168 · Cited by 260
Self-citationCrino, P. B., & Eberwine, J. H. (1996) · Neuron · doi:10.1016/s0896-6273(00)80248-2 · Cited by 257
Corbin, F. (1997) · Human Molecular Genetics · doi:10.1093/hmg/6.9.1465 · Cited by 239
Rickman, C. B., Li, T., Frankel, W. N., Danciger, M., Coffin, J. M., Applebury, M. L., & Farber, D. B. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.7.2955 · Cited by 216
Translational Machinery in Dendrites of Hippocampal Neurons in Culture
Tiedge, H., & Brosius, J. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-22-07171.1996 · Cited by 205
Advances in Molecular Analysis of Fragile X Syndrome
Warren, S. T. (1994) · JAMA · doi:10.1001/jama.1994.03510310066040 · Cited by 204
Torre, E. R., & Steward, O. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-03-00762.1992 · Cited by 204
Greenough, W. T., Hwang, H.-M., & Gorman, C. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.13.4549 · Cited by 195
Self-citationCeman, S., Brown, V., & Warren, S. T. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.12.7925 · Cited by 181
Khandjian, E. W., Huot, M.-E., Tremblay, S., Davidovic, L., Mazroui, R., & Bardoni, B. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0405398101 · Cited by 178
Steward, O., & Falk, P. (1986) · Journal of Neuroscience · doi:10.1523/jneurosci.06-02-00412.1986 · Cited by 173
FMRP is associated to the ribosomes via RNA
Tamanini, F. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.6.809 · Cited by 143
Identification of sites for exponential translation in living dendrites
Job, C., & Eberwine, J. (2001) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.231485698 · Cited by 135
Hagerman, R. J., & Hagerman, P. J. (2002) · Cambridge University Press eBooks · doi:10.1017/cbo9780511543876.009 · Cited by 108
Irwin, S. A., Swain, R. A., Christmon, C. A., Chakravarti, A. B., Weiler, I. J., & Greenough, W. T. (2000) · Neurobiology of Learning and Memory · doi:10.1006/nlme.1999.3914 · Cited by 84
Self-citationRNAs That Interact with the Fragile X Syndrome RNA Binding Protein FMRP
Sung, Y. J., Conti, J., Currie, J. R., Brown, W. T., & Denman, R. B. (2000) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2000.3405 · Cited by 82
Asaki, C., Usuda, N., Nakazawa, A., Kametani, K., & Suzuki, T. (2003) · Brain Research · doi:10.1016/s0006-8993(03)02523-x · Cited by 78
Todd, P. K., & Mack, K. J. (2000) · Molecular Brain Research · doi:10.1016/s0169-328x(00)00098-x · Cited by 69
Klintsova, A. Y., Matthews, J. T., Goodlett, C. R., Napper, R. M. A., & Greenough, W. T. (1997) · Alcoholism Clinical and Experimental Research · doi:10.1111/j.1530-0277.1997.tb04446.x · Cited by 55
Self-citationIrwin (2001) · doi:10.1002/1096-8628(20010115)98:2
Self-citation
Research works printed but not linked: 3
- Stefani, G., Fraser, C. E., Darnell, J. C. & Darnell, R. B. (2004) J. Neurosci. 24, 9272–9276.
- Bakker, C. E., Verheij, C., Willemsen, R., van der Helm, R., Oerlemans, F., Vermey, M., Bygrave, A., Hoogeveen, A. T., Oostra, B. A., Reyniers, E., et al. (1994) Cell 78, 23–33.8033209
- Klintsova, A. Y., Matthews, J. T., Goodlett, C. R., Napper, R. M. & Greenough, W. T. (1997) Alcohol. Clin. Exp. Res. 21, 1257–1263.9347087
Cited by
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Trinucleotide Repeat Disorders
Orr, H. T., & Zoghbi, H. Y. (2007) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.29.051605.113042 · Cited by 1,394
Fragile X Syndrome: Loss of Local mRNA Regulation Alters Synaptic Development and Function
Bassell, G. J., & Warren, S. T. (2008) · Neuron · doi:10.1016/j.neuron.2008.10.004 · Cited by 1,051
Hagerman, R. J., Berry-Kravis, E., Hazlett, H. C., Bailey, D. B., Moine, H., Kooy, R. F., Tassone, F., Gantois, I., Sonenberg, N., Mandel, J. L., et al. (2017) · Nature Reviews Disease Primers · doi:10.1038/nrdp.2017.65 · Cited by 838
Advances in the Treatment of Fragile X Syndrome
Hagerman, R. J., Berry-Kravis, E., Kaufmann, W. E., Ono, M. Y., Tartaglia, N., Lachiewicz, A., Kronk, R., Delahunty, C., Hessl, D., Visootsak, J., et al. (2009) · Pediatrics · doi:10.1542/peds.2008-0317 · Cited by 596
Park, S., Park, J. M., Kim, S., Kim, J.-A., Shepherd, J. D., Smith-Hicks, C. L., Chowdhury, S., Kaufmann, W., Kuhl, D., Ryazanov, A. G., et al. (2008) · Neuron · doi:10.1016/j.neuron.2008.05.023 · Cited by 516
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Metadata
- Authors
- Weiler, I. J., Spangler, C. C., Klintsova, A. Y., Grossman, A. W., Kim, S. H., Bertaina-Anglade, V., Khaliq, H., de Vries, F. E., Lambers, F. A. E., Hatia, F., Base, C. K., & Greenough, W. T.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Beckman Institute, Neuroscience Program, and Departments of Psychology, Psychiatry, and Cell and Structural Biology, University of Illinois at Urbana–Champaign, Urbana, IL 61801
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2004-11-17
- Volume
- 101
- Issue
- 50
- Pages
- 17504-17509
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC536018
- OpenAlex
- W2007532395
- Cited by
- 210
- References
- 52
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 3 · 2022: 7 · 2021: 4 · 2020: 4 · 2019: 4 · 2018: 4 · 2017: 8 · 2016: 4 · 2015: 10 · 2014: 7 · 2013: 7 · 2012: 16
- FWCI
- 11.25
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Pure Amsterdam UMC (publishedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Funders
- FRAXA Research Foundation (MH35321)
- Universiteit Antwerpen
- National Institutes of Health (5r03hd035565-02, 5r01hd037175-03)
- National Institute of Mental Health (MH-35321, R01MH035321, R37MH035321)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R03 HD 35565-01, HD 37175)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- FMRP, fragile X syndrome, FMR1 gene, synaptic protein synthesis, local translation, group I metabotropic glutamate receptors, protein kinase C, dendritic spines, synaptoneurosomes, visual cortex, Fmr1 knockout mice, RNA-binding proteins
- MeSH terms
- Animals, Glycine, Methionine, Microscopy, Electron, Nerve Tissue Proteins, Potassium, Resorcinols, Ribosomes, Synapses, Protein Biosynthesis, RNA-Binding Proteins, Gene Deletion, Mice, Knockout, Neurotransmitter Agents, Mice, Fragile X Messenger Ribonucleoprotein 1, Fragile X Mental Retardation Protein
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