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Cited by 159Herpesvirus Infections and TreatmentsAnimal Virus Infections StudiesCytomegalovirus and herpesvirus research

Cytoskeletal rearrangements and cell extensions induced by the US3 kinase of an alphaherpesvirus are associated with enhanced spread

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Favoreel, Herman W., Van Minnebruggen, Geert, Adriaensen, Dirk, Nauwynck, Hans J.·2005·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0409099102PMC PMC1157013
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Abstract

The US3 protein is a viral kinase that is conserved among the Alphaherpesvirinae. Here, we show that US3 of the swine alphaherpesvirus pseudorabies virus causes dramatic alterations in the cytoskeleton, resulting in the formation of long actin- and microtubule-containing cell projections in infected and transfected cells. Analysis with a GFP-labeled virus showed that multiple virus particles move inside the projections toward the tip. GFP-labeled virus could also be found in the cytoplasm of neighboring cells that were in contact with the projections. In addition, projection formation could be inhibited by using the actin-stabilizing drug jasplakinolide and could be induced by using the Rho kinase inhibitor Y27632. Analyzing the effect of these drugs on intercellular virus spread indicated that the observed US3-induced alterations in the host cytoskeleton are associated with enhanced intercellular virus spread, thereby suggesting a previously undescribed aspect of alphaherpesvirus spread.

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  • Cell-cell fusion, synapse formation, filopodial bridges, and nanotubes have been documented for cytomegaloviruses, varicella zoster virus, pseudorabies virus, and herpes simplex virus.

    “Cell-cell fusion, synapse formation, filopodial bridges, and nanotubes were documented for cytomegaloviruses (CMV), varicella zoster virus (VZV), pseudorabies virus (PRV), and herpes simplex virus (HSV-1) (Cole and Grose, 2003; Dingwell et al., 1994; Favoreel et al., 2005; Gerna et al., 2000; Sweet, 1999; van Leeuwen et al., 2002).”

    From Equid Herpesvirus-1 Exploits the Extracellular Matrix of Mononuclear Cells to Ensure Transport to Target Cells (2020) · cites this paper as Favoreel et al., 2005

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Authors
Favoreel, H. W., Van Minnebruggen, G., Adriaensen, D., & Nauwynck, H. J.
Affiliations
  • University of Antwerp (BE)
  • Laboratory of Virology and Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium; and Laboratory of Cell Biology and Histology, Department of Biomedical Sciences, University of Antwerp, 2020 Antwerp, Belgium
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2005-06-10
Volume
102
Issue
25
Pages
8990-8995
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0409099102
PMC
PMC1157013
OpenAlex
W2039667276
Cited by
159
References
39
Citations by year
2026: 5 · 2025: 5 · 2024: 1 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 7 · 2019: 5 · 2018: 7 · 2017: 8 · 2016: 8 · 2015: 4 · 2014: 5 · 2013: 7 · 2012: 3
FWCI
9.56
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion)
Topics
  • Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
Keywords
pseudorabies virus, alphaherpesvirus, cytoskeletal rearrangement, microtubules, jasplakinolide, Rho kinase inhibition, Y-27632, host cell cytoskeleton
MeSH terms
Amides, Animals, Capsid, Cytoskeleton, Male, Microtubules, Viral Plaque Assay, Herpesvirus 1, Suid, Pyridines, Swine, Testis, Protein Serine-Threonine Kinases, Microscopy, Confocal, Depsipeptides

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