Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus
Open accessAbstract
Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. Using biochemical assays, immunofluorescence, and immunoelectron microscopy in the presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules. Antibody labeling revealed the attachment of dynein, a minus end-directed, microtubule-dependent motor, to the viral capsids. We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus.
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Viruses such as herpes simplex virus and some serotypes of adenovirus could travel through cytoplasm via microtubule-mediated transport.
“Virus such as herpes simplex virus and some serotypes of adenovirus could travel through the cytoplasm via microtubule-mediated transport [76, 193, 194].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [193]
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- Authors
- Sodeik, B., Ebersold, M. W., & Helenius, A.
- Affiliations
- Yale University (US)
- Corresponding author
- Sodeik, Beate
- ORCID
- Sodeik, Beate
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1997-03-10
- Volume
- 136
- Issue
- 5
- Pages
- 1007-1021
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2132479
- OpenAlex
- W2147890616
- Cited by
- 687
- References
- 68
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 12 · 2023: 12 · 2022: 16 · 2021: 19 · 2020: 15 · 2019: 21 · 2018: 17 · 2017: 17 · 2016: 18 · 2015: 22 · 2014: 30 · 2013: 26 · 2012: 24
- FWCI
- 24.98
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI 18599)
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Keywords
- herpes simplex virus type 1, microtubules, dynein, nuclear import, nuclear pore complex, Vero cells, microtubule depolymerization
- MeSH terms
- Animals, Biological Transport, Capsid, Cell Nucleus, Chlorocebus aethiops, Cytoplasm, Dyneins, Actin Cytoskeleton, Microtubules, Vero Cells, Cytochalasin D, Nocodazole, Paclitaxel, Immediate-Early Proteins, Herpesvirus 1, Human, Capsid Proteins
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