Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission
Abstract
Transmission of HIV-1 via intercellular connections has been estimated as 100-1000 times more efficient than a cell-free process, perhaps in part explaining persistent viral spread in the presence of neutralizing antibodies. Such effective intercellular transfer of HIV-1 could occur through virological synapses or target-cell filopodia connected to infected cells. Here we report that membrane nanotubes, formed when T cells make contact and subsequently part, provide a new route for HIV-1 transmission. Membrane nanotubes are known to connect various cell types, including neuronal and immune cells, and allow calcium-mediated signals to spread between connected myeloid cells. However, T-cell nanotubes are distinct from open-ended membranous tethers between other cell types, as a dynamic junction persists within T-cell nanotubes or at their contact with cell bodies. We also report that an extracellular matrix scaffold allows T-cell nanotubes to adopt variably shaped contours. HIV-1 transfers to uninfected T cells through nanotubes in a receptor-dependent manner. These data lead us to propose that HIV-1 can spread using nanotubular connections formed by short-term intercellular unions in which T cells specialize.
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HIV-1 and HTLV-1 cell-to-cell transmission via TNT has been reported.
“Later, HIV-1 and HTLV-1 cell-to-cell transmission via TNT was reported.”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [108]
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- Authors
- Sowinski, S., Jolly, C., Berninghausen, O., Purbhoo, M. A., Chauveau, A., Köhler, K., Oddos, S., Eissmann, P., Brodsky, F. M., Hopkins, C., Önfelt, B., Sattentau, Q., & Davis, D. M.
- Affiliations
- Imperial College London (GB)
- University of Oxford (GB)
- University of California, San Francisco (US)
- Karolinska Institutet (SE)
- Corresponding author
- Davis, Daniel M.
- ORCID
- Venue
- Nature Cell Biology
- Published
- 2008-01-13
- Volume
- 10
- Issue
- 2
- Pages
- 211-219
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2088757776
- Cited by
- 754
- References
- 31
- Citations by year
- 2026: 22 · 2025: 19 · 2024: 30 · 2023: 24 · 2022: 38 · 2021: 45 · 2020: 47 · 2019: 37 · 2018: 46 · 2017: 41 · 2016: 48 · 2015: 35 · 2014: 38 · 2013: 32 · 2012: 58
- FWCI
- 20.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council (BB/D011663/1)
- National Institute of Allergy and Infectious Diseases (P01AI064520)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- tunneling nanotubes, HIV-1 transmission, T cells, cell-cell contact, virological synapse, extracellular matrix scaffolds, calcium signaling
- MeSH terms
- Humans, Intercellular Junctions, T-Lymphocytes, Virulence, HIV-1, Jurkat Cells, Cell Surface Extensions
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