The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells
Open accessAbstract
ES cells represent a valuable model for investigating early embryo development and hold promise for future regenerative medicine strategies. The self-renewal of pluripotent mouse ES cells has been shown to require extrinsic stimulation by the bone morphogenetic protein (BMP) and leukemia inhibitory factor signaling pathways and the expression of the transcription factors Oct4 and Nanog. However, the network of interactions among extrinsic and intrinsic determinants of ES cell pluripotency is currently poorly understood. Here, we show that Nanog expression is up-regulated in mouse ES cells by the binding of T (Brachyury) and STAT3 to an enhancer element in the mouse Nanog gene. We further show that Nanog blocks BMP-induced mesoderm differentiation of ES cells by physically interacting with Smad1 and interfering with the recruitment of coactivators to the active Smad transcriptional complexes. Taken together, our findings illustrate the existence of ES cell-specific regulatory networks that underlie the maintenance of ES cell pluripotency and provide mechanistic insights into the role of Nanog in this process.
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Citations
Cites
Targeted mutation of the DNA methyltransferase gene results in embryonic lethality
Li, E., Bestor, T. H., & Jaenisch, R. (1992) · Cell · doi:10.1016/0092-8674(92)90611-f · Cited by 3,984
Cited by
Nanog Is the Gateway to the Pluripotent Ground State
Silva, J., Nichols, J., Theunissen, T. W., Guo, G., van Oosten, A. L., Barrandon, O., Wray, J., Yamanaka, S., Chambers, I., & Smith, A. (2009) · Cell · doi:10.1016/j.cell.2009.07.039 · Cited by 1,031
Samavarchi-Tehrani, P., Golipour, A., David, L., Sung, H.-K., Beyer, T. A., Datti, A., Woltjen, K., Nagy, A., & Wrana, J. L. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.04.015 · Cited by 943
The regulation of TGFβ signal transduction
Moustakas, A., & Heldin, C.-H. (2009) · Development · doi:10.1242/dev.030338 · Cited by 832
A Small-Molecule Inhibitor of Tgf-β Signaling Replaces Sox2 in Reprogramming by Inducing Nanog
Ichida, J. K., Blanchard, J., Lam, K., Son, E. Y., Chung, J. E., Egli, D., Loh, K. M., Carter, A. C., Di Giorgio, F. P., Koszka, K., et al. (2009) · Cell Stem Cell · doi:10.1016/j.stem.2009.09.012 · Cited by 808
How is pluripotency determined and maintained?
Niwa, H. (2007) · Development · doi:10.1242/dev.02787 · Cited by 783
Metadata
- Authors
- Suzuki, A., Raya, Á., Kawakami, Y., Morita, M., Matsui, T., Nakashima, K., Gage, F. H., Rodríguez-Esteban, C., & Izpisúa Belmonte, J. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-07-05
- Volume
- 103
- Issue
- 27
- Pages
- 10294-10299
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2134128560
- Cited by
- 273
- References
- 1
- FWCI
- 10.31
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells. https://app.cochat.ai/papers/10.1073/pnas.0506945103
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- Suzuki, A., Raya, Á., Kawakami, Y., Morita, M., Matsui, T., Nakashima, K., Gage, F. H., Rodríguez-Esteban, C., & Izpisúa Belmonte, J. C. (2006). Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0506945103
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