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Cited by 262

The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.

Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis

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Lukowitz, Wolfgang, Nickle, Todd C., Meinke, David W., Last, Robert L., Conklin, Patricia L., Somerville, Christopher R.·2001·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.051625798
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Abstract

Arabidopsis cyt1 mutants have a complex phenotype indicative of a severe defect in cell wall biogenesis. Mutant embryos arrest as wide, heart-shaped structures characterized by ectopic accumulation of callose and the occurrence of incomplete cell walls. Texture and thickness of the cell walls are irregular, and unesterified pectins show an abnormally diffuse distribution. To determine the molecular basis of these defects, we have cloned the CYT1 gene by a map-based approach and found that it encodes mannose-1-phosphate guanylyltransferase. A weak mutation in the same gene, called vtc1 , has previously been identified on the basis of ozone sensitivity due to reduced levels of ascorbic acid. Mutant cyt1 embryos are deficient in N-glycosylation and have an altered composition of cell wall polysaccharides. Most notably, they show a 5-fold decrease in cellulose content. Characteristic aspects of the cyt1 phenotype, including radial swelling and accumulation of callose, can be mimicked with the inhibitor of N-glycosylation, tunicamycin. Our results suggest that N-glycosylation is required for cellulose biosynthesis and that a deficiency in this process can account for most phenotypic features of cyt1 embryos.

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Metadata

Authors
Lukowitz, W., Nickle, T. C., Meinke, D. W., Last, R. L., Conklin, P. L., & Somerville, C. R.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2001-02-20
Volume
98
Issue
5
Pages
2262-2267
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.051625798
OpenAlex
W2076077373
Cited by
262
References
37
FWCI
22.07
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis. https://app.cochat.ai/papers/10.1073/pnas.051625798
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Lukowitz, W., Nickle, T. C., Meinke, D. W., Last, R. L., Conklin, P. L., & Somerville, C. R. (2001). Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.051625798

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