The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect the social amoeba Dictyostelium discoideum

Pukatzki, Stefan; Kessin, Richard H.; Mekalanos, John J.

doi:10.1073/pnas.052704399

Cited by 289Bacterial biofilms and quorum sensingLegionella and Acanthamoeba researchEscherichia coli research studies

The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect the social amoeba Dictyostelium discoideum

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Pukatzki, Stefan, Kessin, Richard H., Mekalanos, John J.·2002·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.052704399PMC PMC122489

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Abstract

Genetically accessible host models are useful for studying microbial pathogenesis because they offer the means to identify novel strategies that pathogens use to evade immune mechanisms, cause cellular injury, and induce disease. We have developed conditions under which the human pathogen Pseudomonas aeruginosa infects Dictyostelium discoideum , a genetically tractable eukaryotic organism. When D. discoideum is plated on nutrient agar plates with different P. aeruginosa strains, the bacteria form lawns on these plates with amoebae embedded in them. Virulent P. aeruginosa strains kill these amoebae and leave an intact bacterial lawn. A number of P. aeruginosa mutants have been identified that are avirulent in this assay. Amoebae feed on these bacteria and form plaques in their bacterial lawns. One avirulent mutant strain carries an insertional mutation in the lasR gene. LasR is a transcription factor that controls a number of virulence genes in a density-dependent fashion. Another class of avirulent P. aeruginosa mutants is defective in type III secretion. One mutant lacks the PscJ protein, a structural component of the secretion apparatus, suggesting that cytotoxins are injected into the D. discoideum cell. One of these cytotoxins is ExoU, and exoU mutants are avirulent toward D. discoideum . Complementation of the lasR and exoU mutations restores virulence. Therefore, P. aeruginosa uses conserved virulence pathways to kill D. discoideum .

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Several bacterial pathogens for humans, such as legionella, mycobacteria and salmonella, are pathogenic also for Dictyostelium.
“Several bacterial pathogens for humans, such as legionella, mycobacteria and salmonella, are pathogenic also for Dictyostelium, favouring the use of this model to study host-pathogen interactions [9-19].”
From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [13]

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The human pathogen Pseudo utilizes conserved virulence ( the social amoeba Dictyostel
Pukatzki, S. U., Kessin, R. H., & Mekalanos, J. J. (2016)
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Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model
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Authors: Pukatzki, S., Kessin, R. H., & Mekalanos, J. J.
Affiliations: Harvard University (US)
ORCID: Pukatzki, Stefan
Mekalanos, John J.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2002-02-26
Volume: 99
Issue: 5
Pages: 3159-3164
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.052704399
PMC: PMC122489
OpenAlex: W2104817697
Cited by: 289
References: 42
Citations by year: 2026: 3 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 6 · 2021: 10 · 2020: 12 · 2019: 9 · 2018: 16 · 2017: 5 · 2016: 10 · 2015: 8 · 2014: 18 · 2013: 13 · 2012: 14
FWCI: 6.74
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Cystic Fibrosis Foundation
National Institutes of Health
University of California, San Francisco
National Institute of General Medical Sciences (R01GM033136, GM33136-20)
National Institute of Allergy and Infectious Diseases (R01AI026289, AI-26289)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Legionella and Acanthamoeba research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
Keywords: Pseudomonas aeruginosa, Dictyostelium discoideum, bacterial pathogenesis, virulence factors, type III secretion system, avirulent mutants, host-pathogen interaction, density dependence
MeSH terms: Animals, Bacterial Proteins, Bacterial Toxins, Cytotoxins, Dictyostelium, DNA-Binding Proteins, Humans, Phagocytosis, Pseudomonas aeruginosa, Soil Microbiology, Virulence, Trans-Activators

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The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect the social amoeba Dictyostelium discoideum

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