The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
The dominant role of CD8+dendritic cells in cross-presentation is not dictated by antigen capture
Open accessAbstract
Mouse spleens contain three populations of conventional (CD11c high) dendritic cells (DCs) that play distinct functions. The CD8+DC are unique in that they can present exogenous antigens on their MHC class I molecules, a process known as cross-presentation. It is unclear whether this special ability is because only the CD8+DC can capture the antigens used in cross-presentation assays, or because this is the only DC population that possesses specialized machinery for cross-presentation. To solve this important question we examined the splenic DC subsets for their ability to both present via MHC class II molecules and cross-present via MHC class I using four different forms of the model antigen ovalbumin (OVA). These forms include a cell-associated form, a soluble form, OVA expressed in bacteria, or OVA bound to latex beads. With the exception of bacterial antigen, which was poorly cross-presented by all DC, all antigenic forms were cross-presented much more efficiently by the CD8+DC. This pattern could not be attributed simply to a difference in antigen capture because all DC subsets presented the antigen via MHC class II. Indeed, direct assessments of endocytosis showed that CD8+and CD8−DC captured comparable amounts of soluble and bead-associated antigen, yet only the CD8+DC cross-presented these antigenic forms. Our results indicate that cross-presentation requires specialized machinery that is expressed by CD8+DC but largely absent from CD8−DC. This conclusion has important implications for the design of vaccination strategies based on antigen targeting to DC.
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T Helper 17 Cells Promote Cytotoxic T Cell Activation in Tumor Immunity
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Metadata
- Authors
- Schnorrer, P., Behrens, G. M. N., Wilson, N. S., Pooley, J. L., Smith, C. M., El-Sukkari, D., Davey, G., Kupresanin, F., Li, M., Maraskovsky, E., Belz, G. T., Carbone, F. R., Shortman, K., Heath, W. R., & Villadangos, J. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-07-11
- Volume
- 103
- Issue
- 28
- Pages
- 10729-10734
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2021943943
- Cited by
- 383
- References
- 44
- FWCI
- 12.53
- Type
- journal article
- Language
- en
- Open access
- green
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- Schnorrer, P., Behrens, G. M. N., Wilson, N. S., Pooley, J. L., Smith, C. M., El-Sukkari, D., Davey, G., Kupresanin, F., Li, M., Maraskovsky, E., Belz, G. T., Carbone, F. R., Shortman, K., Heath, W. R., & Villadangos, J. A. (2006). The dominant role of CD8+dendritic cells in cross-presentation is not dictated by antigen capture. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0601956103
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