The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing
Abstract
An efficient, nanoliter-scale microfabricated bioprocessor integrating all three Sanger sequencing steps, thermal cycling, sample purification, and capillary electrophoresis, has been developed and evaluated. Hybrid glass–polydimethylsiloxane (PDMS) wafer-scale construction is used to combine 250-nl reactors, affinity-capture purification chambers, high-performance capillary electrophoresis channels, and pneumatic valves and pumps onto a single microfabricated device. Lab-on-a-chip-level integration enables complete Sanger sequencing from only 1 fmol of DNA template. Up to 556 continuous bases were sequenced with 99% accuracy, demonstrating read lengths required for de novo sequencing of human and other complex genomes. The performance of this miniaturized DNA sequencer provides a benchmark for predicting the ultimate cost and efficiency limits of Sanger sequencing.
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Cites
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Self-citationGenome Sequence of the Nematode C. elegans : A Platform for Investigating Biology
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Cited by
bold : The Barcode of Life Data System (http://www.barcodinglife.org)
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Next-generation DNA sequencing
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Control and detection of chemical reactions in microfluidic systems
deMello, A. J. (2006) · Nature · doi:10.1038/nature05062 · Cited by 1,495
Microfluidic Devices for Bioapplications
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Metadata
- Authors
- Blazej, R. G., Kumaresan, P., & Mathies, R. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-05-09
- Volume
- 103
- Issue
- 19
- Pages
- 7240-7245
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2089976786
- Cited by
- 277
- References
- 23
- FWCI
- 27.06
- Type
- journal article
- Language
- en
- Open access
- closed
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- Blazej, R. G., Kumaresan, P., & Mathies, R. A. (2006). Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0602476103
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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