Next-generation DNA sequencing
Abstract
DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection. Over the past three years, massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and democratizing the field by putting the sequencing capacity of a major genome center in the hands of individual investigators. These new technologies are rapidly evolving, and near-term challenges include the development of robust protocols for generating sequencing libraries, building effective new approaches to data-analysis, and often a rethinking of experimental design. Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes, transcriptomes and interactomes to become inexpensive, routine and widespread, rather than requiring significant production-scale efforts.
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Short-read-only assemblies often struggle with repetitive and intergenic regions, resulting in fragmented assembly and poor access to regulatory and promoter sequences.
“However, short-read-only assemblies often struggle with repetitive and intergenic regions, resulting in fragmented assembly and poor access to regulatory and promoter sequences5,6.”
From Hybrid genome assembly and annotation of Danionella translucida (2019) · cites this paper as [5]
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- Authors
- Shendure, J., & Ji, H.
- Affiliations
- University of Washington (US)
- Stanford Medicine (US)
- Corresponding author
- Shendure, Jay
- Ji, Hanlee
- Venue
- Nature Biotechnology
- Published
- 2008-10
- Volume
- 26
- Issue
- 10
- Pages
- 1135-1145
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2061680337
- Cited by
- 4,509
- References
- 6
- Citations by year
- 2026: 71 · 2025: 146 · 2024: 176 · 2023: 133 · 2022: 159 · 2021: 180 · 2020: 203 · 2019: 188 · 2018: 270 · 2017: 284 · 2016: 302 · 2015: 330 · 2014: 372 · 2013: 367 · 2012: 382
- FWCI
- 128.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- DigitalCommons-IMSA (Illinois Mathematics and Science Academy) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genetics, Bioinformatics, and Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomics and Rare Diseases
- Keywords
- next-generation sequencing, sequencing cost reduction, library preparation, sequencing data analysis, experimental design, genome analysis, gene expression profiling, interactome analysis
- MeSH terms
- Chromosome Mapping, Forecasting, Sequence Alignment, Sequence Analysis, DNA, Genomics
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