Cited by 367Blood properties and coagulationBarrier Structure and Function StudiesAcute Ischemic Stroke Management

Penetrating arterioles are a bottleneck in the perfusion of neocortex

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Nishimura, Nozomi, Schaffer, Chris B., Friedman, Beth, Lyden, Patrick D., Kleinfeld, David·2007·Proceedings of the National Academy of Sciences

Abstract

Penetrating arterioles bridge the mesh of communicating arterioles on the surface of cortex with the subsurface microvascular bed that feeds the underlying neural tissue. We tested the conjecture that penetrating arterioles, which are positioned to regulate the delivery of blood, are loci of severe ischemia in the event of occlusion. Focal photothrombosis was used to occlude single penetrating arterioles in rat parietal cortex, and the resultant changes in flow of red blood cells were measured with two-photon laser-scanning microscopy in individual subsurface microvessels that surround the occlusion. We observed that the average flow of red blood cells nearly stalls adjacent to the occlusion and remains within 30% of its baseline value in vessels as far as 10 branch points downstream from the occlusion. Preservation of average flow emerges 350 μm away; this length scale is consistent with the spatial distribution of penetrating arterioles. We conclude that penetrating arterioles are a bottleneck in the supply of blood to neocortex, at least to superficial layers.

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Crossref · 10.1073/pnas.0609551104

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Authors
Nishimura, N., Schaffer, C. B., Friedman, B., Lyden, P. D., & Kleinfeld, D.
Affiliations
  • University of California San Diego (US)
  • Neurosciences,
Corresponding author
Kleinfeld, David
Venue
Proceedings of the National Academy of Sciences
Published
2007-01-02
Volume
104
Issue
1
Pages
365-370
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2036472881
Cited by
367
References
32
Citations by year
2026: 13 · 2025: 13 · 2024: 14 · 2023: 22 · 2022: 18 · 2021: 30 · 2020: 23 · 2019: 28 · 2018: 17 · 2017: 20 · 2016: 21 · 2015: 19 · 2014: 15 · 2013: 28 · 2012: 21
FWCI
10.05
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute of Neurological Disorders and Stroke (NS/043300, R01NS043300)
  • National Institute of Biomedical Imaging and Bioengineering (R01EB003832, EB/003832)
  • National Center for Research Resources (RR/021907, R21RR021907)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Blood properties and coagulation
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Health Sciences › Medicine › Epidemiology › Acute Ischemic Stroke Management
Keywords
penetrating arterioles, cerebral blood flow, arteriolar occlusion, ischemia, red blood cell flow, microvascular bed, parietal cortex, two-photon microscopy, cerebral microcirculation
MeSH terms
Animals, Arterioles, Brain Ischemia, Cerebrovascular Circulation, Humans, Male, Models, Cardiovascular, Perfusion, Rats, Sprague-Dawley, Neocortex, Stroke, Rats

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