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Cited by 484

The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.

Spermatogonial stem cells share some, but not all, phenotypic and functional characteristics with other stem cells

Open access
Kubota, Hiroshi, Avarbock, Mary R., Brinster, Ralph L.·2003·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0631767100
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Abstract

Spermatogonial stem cells (SSCs) are responsible for maintaining spermatogenesis throughout life in the male by continuous production of daughter cells that differentiate into spermatozoa. However, no unique phenotypic markers to identify SSCs have been described. In this study, the SSC surface phenotype was characterized by using flow cytometric cell sorting in conjunction with a transplantation functional assay for SSCs. Highly enriched stem cell activity was found in the MHC class I (MHC-I) – Thy-1 + c-kit – cell fraction of the mouse cryptorchid testis. There was little or no stem cell activity in any other fraction. The antigenic phenotype of the MHC-I – Thy-1 + c-kit – SSCs was α6-integrin + CD24 + αvintegrin – Sca-1 – CD34 – . Subsequently, testis side population (SP) cells, which are defined by a Hoechst dye efflux assay, were identified. Their surface phenotype was found to be MHC-I + Thy-1 – Sca-1 + , and the transplantation assay demonstrated that the testis SP and SSCs are distinct populations. In several other tissues, the SP has been shown to contain stem cells, but we found that this characteristic does not define SSCs. The identification of a surface phenotype that allows production of a highly enriched SSC population will facilitate functional and genomic studies and enable further comparison with other stem cells.

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Metadata

Authors
Kubota, H., Avarbock, M. R., & Brinster, R. L.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2003-05-08
Volume
100
Issue
11
Pages
6487-6492
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0631767100
OpenAlex
W1993491850
Cited by
484
References
40
FWCI
21.41
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Spermatogonial stem cells share some, but not all, phenotypic and functional characteristics with other stem cells. https://app.cochat.ai/papers/10.1073/pnas.0631767100
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Kubota, H., Avarbock, M. R., & Brinster, R. L. (2003). Spermatogonial stem cells share some, but not all, phenotypic and functional characteristics with other stem cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0631767100

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.