Cited by 477Genetic and Kidney Cyst DiseasesBone Metabolism and DiseasesCellular Mechanics and Interactions

The DOI matches its Crossref record; 102 of the 102 references it cites were found in a registry or an index.

Primary cilia mediate mechanosensing in bone cells by a calcium-independent mechanism

Open access

Malone, Amanda M. D., Anderson, Charles T., Tummala, Padmaja, Kwon, Ronald Y., Johnston, Tyler R., Stearns, Tim, Jacobs, Christopher R.·2007·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.0700636104PMC PMC1939687

Open this paper in CoChat — free account

Abstract

Primary cilia are sensory organelles that translate extracellular chemical and mechanical cues into cellular responses. Bone is an exquisitely mechanosensitive organ, and its homeostasis depends on the ability of bone cells to sense and respond to mechanical stimuli. One such stimulus is dynamic fluid flow, which triggers biochemical and transcriptional changes in bone cells by an unknown mechanism. Here we report that bone cells possess primary cilia that project from the cell surface and deflect during fluid flow and that these primary cilia are required for osteogenic and bone resorptive responses to dynamic fluid flow. We also show that, unlike in kidney cells, primary cilia in bone translate fluid flow into cellular responses in bone cells independently of Ca 2+ flux and stretch-activated ion channels. These results suggest that primary cilia might regulate homeostasis in diverse tissues by allowing mechanical signals to alter cellular activity via tissue-specific pathways. Our identification of a mechanism for mechanotransduction in bone could lead to therapeutic approaches for combating bone loss due to osteoporosis and disuse.

Integrity signals

Registry

Crossref · 10.1073/pnas.0700636104

Cites

102 of 102 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Isolation of Putative Progenitor Endothelial Cells for Angiogenesis
Asahara, T., Murohara, T., Sullivan, A., Silver, M., van der Zee, R., Li, T., Witzenbichler, B., Schatteman, G., & Isner, J. M. (1997) · Science · doi:10.1126/science.275.5302.964 · Cited by 8,725
Bone Marrow Origin of Endothelial Progenitor Cells Responsible for Postnatal Vasculogenesis in Physiological and Pathological Neovascularization
Asahara, T., Masuda, H., Takahashi, T., Kalka, C., Pastore, C., Silver, M., Kearne, M., Magner, M., & Isner, J. M. (1999) · Circulation Research · doi:10.1161/01.res.85.3.221 · Cited by 3,263
Flow-mediated endothelial mechanotransduction
Davies, P. F. (1995) · Physiological Reviews · doi:10.1152/physrev.1995.75.3.519 · Cited by 2,802
Neovascularization of ischemic myocardium by human bone-marrow–derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function
Kocher, A. A., Schuster, M., Szabolcs, M., Takuma, S., Burkhoff, D., Wang, J., Homma, S., Edwards, N. M., & Itescu, S. (2001) · Nature Medicine · doi:10.1038/86498 · Cited by 2,524
Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization
Takahashi, T., Kalka, C., Masuda, H., Chen, D., Silver, M., Kearney, M., Magner, M., Isner, J. M., & Asahara, T. (1999) · Nature Medicine · doi:10.1038/7434 · Cited by 2,421
Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursors
Peichev, M., Naiyer, A. J., Pereira, D., Zhu, Z., Lane, W. J., Williams, M., Oz, M., Hicklin, D. J., Witte, L., Moore, M. A. S., et al. (2000) · Blood · doi:10.1182/blood.v95.3.952.003k27_952_958 · Cited by 2,268
Polycystins 1 and 2 mediate mechanosensation in the primary cilium of kidney cells
Nauli, S. M., Alenghat, F. J., Luo, Y., Williams, E., Vassilev, P., Li, X., Elia, A. E. H., Lu, W., Brown, E. M., Quinn, S. J., et al. (2003) · Nature Genetics · doi:10.1038/ng1076 · Cited by 2,057
Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization
Kalka, C., Masuda, H., Takahashi, T., Kalka-Moll, W. M., Silver, M., Kearney, M., Li, T., Isner, J. M., & Asahara, T. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.7.3422 · Cited by 1,862
Impaired recruitment of bone-marrow–derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth
Lyden, D., Hattori, K., Dias, S., Costa, C., Blaikie, P., Butros, L., Chadburn, A., Heissig, B., Marks, W., Witte, L., et al. (2001) · Nature Medicine · doi:10.1038/nm1101-1194 · Cited by 1,860
VEGF contributes to postnatal neovascularization by mobilizing bone marrow‐derived endothelial progenitor cells
Asahara, T., Takahashi, T., Masuda, H., Kalka, C., Chen, D., Iwaguro, H., Inai, Y., Silver, M., & Isner, J. M. (1999) · The EMBO Journal · doi:10.1093/emboj/18.14.3964 · Cited by 1,851

Show all 102 references

Research works printed but not linked: 2

GC Reilly, TR Haut, CE Yellowley, HJ Donahue, CR Jacobs Biorheology 40, 591–603 (2003).
10.1097/00006534-200301000-00037 · DOI

Sorted by citations

Items 101–200 of 474

Primary Cilia: Cellular Sensors for the Skeleton
Anderson, C. T., Castillo, A. B., Brugmann, S. A., Helms, J. A., Jacobs, C. R., & Stearns, T. (2008) · The Anatomical Record · doi:10.1002/ar.20754 · Cited by 70
Self-citation
Sending mixed signals: Cilia-dependent signaling during development and disease
Elliott, K. H., & Brugmann, S. A. (2018) · Developmental Biology · doi:10.1016/j.ydbio.2018.03.007 · Cited by 74
Primary cilia are highly oriented with respect to collagen direction and long axis of extensor tendon
Donnelly, E. L., Ascenzi, M., & Farnum, C. E. (2009) · Journal of Orthopaedic Research® · doi:10.1002/jor.20946 · Cited by 73
Mechanobiological Modulation of Cytoskeleton and Calcium Influx in Osteoblastic Cells by Short-Term Focused Acoustic Radiation Force
Zhang, S., Cheng, J., & Qin, Y. (2012) · PLoS ONE · doi:10.1371/journal.pone.0038343 · Cited by 71
The G protein-coupled receptor Gpr161 regulates forelimb formation, limb patterning and skeletal morphogenesis in a primary cilium-dependent manner
Hwang, S.-H., White, K. A., Somatilaka, B. N., Shelton, J. M., Richardson, J. A., & Mukhopadhyay, S. (2018) · Development · doi:10.1242/dev.154054 · Cited by 60
TGFβ1 – induced recruitment of human bone mesenchymal stem cells is mediated by the primary cilium in a SMAD3-dependent manner
Labour, M.-N., Riffault, M., Christensen, S. T., & Hoey, D. A. (2016) · Scientific Reports · doi:10.1038/srep35542 · Cited by 68
The effect of bioreactor induced vibrational stimulation on extracellular matrix production from human derived fibroblasts
Wolchok, J. C., Brokopp, C. E., Underwood, C. J., & Tresco, P. A. (2008) · Biomaterials · doi:10.1016/j.biomaterials.2008.08.035 · Cited by 69
Biological Basis of Bone Formation, Remodeling, and Repair—Part III: Biomechanical Forces
Allori, A. C., Sailon, A. M., Pan, J. H., & Warren, S. M. (2008) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2008.0084 · Cited by 67
Pulsed electromagnetic fields promote osteoblast mineralization and maturation needing the existence of primary cilia
Yan, J., Zhou, J., Ma, H., Ma, X., Gao, Y., Shi, W., Fang, Q.-Q., Ren, Q., Xian, C. J., & Chen, K. (2015) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2015.01.031 · Cited by 66
Primary Cilia and Intraflagellar Transport Proteins in Bone and Cartilage
Yuan, X., & Yang, S. (2016) · Journal of Dental Research · doi:10.1177/0022034516652383 · Cited by 65
Generation of special autosomal dominant polycystic kidney disease iPSCs with the capability of functional kidney-like cell differentiation
Huang, J., Zhou, S., Niu, X., Hu, B., Li, Q., Zhang, F., Zhang, X., Cai, X., Lou, Y., Liu, F., et al. (2017) · Stem Cell Research & Therapy · doi:10.1186/s13287-017-0645-8 · Cited by 65
Adipose-Derived Stem Cells in Functional Bone Tissue Engineering: Lessons from Bone Mechanobiology
Bodle, J. C., Hanson, A. D., & Loboa, E. G. (2011) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2010.0738 · Cited by 63
Bone adaptation: Safety factors and load predictability in shaping skeletal form
Willie, B. M., Zimmermann, E. A., Vitienes, I., Main, R. P., & Komarova, S. V. (2019) · Bone · doi:10.1016/j.bone.2019.115114 · Cited by 71
Primary Cilia of Odontoblasts: Possible Role in Molar Morphogenesis
Thivichon‐Prince, B., Couble, M., Giamarchi, A., Delmas, P., Franco, B., Romio, L., Struys, T., Lambrichts, I., Ressnikoff, D., Magloire, H., et al. (2009) · Journal of Dental Research · doi:10.1177/0022034509345822 · Cited by 66
The Roles of Primary Cilia in Cardiovascular Diseases
Pala, R., Jamal, M., Alshammari, Q. A., & Nauli, S. M. (2018) · Cells · doi:10.3390/cells7120233 · Cited by 65
Cigarette Smoke Induces the Risk of Metabolic Bone Diseases: Transforming Growth Factor Beta Signaling Impairment via Dysfunctional Primary Cilia Affects Migration, Proliferation, and Differentiation of Human Mesenchymal Stem Cells
Aspera-Werz, R. H., Chen, T., Ehnert, S., Zhu, S., Fröhlich, T., & Nussler, A. K. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20122915 · Cited by 58
Flow-induced prostaglandin E2 release regulates Na and K transport in the collecting duct
Flores, D., Liu, Y., Liu, W., Satlin, L. M., & Rohatgi, R. (2012) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00169.2012 · Cited by 63
Key developments that impacted the field of mechanobiology and mechanotransduction
Wall, M. E., Butler, D. L., Haj, A. J. E., Bodle, J. C., Loboa, E. G., & Banes, A. J. (2017) · Journal of Orthopaedic Research® · doi:10.1002/jor.23707 · Cited by 61
TGF-β1 impairs mechanosensation of human osteoblasts via HDAC6-mediated shortening and distortion of primary cilia
Ehnert, S., Sreekumar, V., Aspera-Werz, R. H., Sajadian, S. O., Wintermeyer, E., Sandmann, G. H., Bahrs, C., Hengstler, J. G., Godoy, P., & Nussler, A. K. (2017) · Journal of Molecular Medicine · doi:10.1007/s00109-017-1526-4 · Cited by 60
Mechanical control of tissue-engineered bone
Hung, B. P., Hutton, D. L., & Grayson, W. L. (2013) · Stem Cell Research & Therapy · doi:10.1186/scrt158 · Cited by 59
Engineering Biomaterials for Regenerative Medicine
Bhatia, S. K. (2011) · Springer eBooks · doi:10.1007/978-1-4614-1080-5 · Cited by 95
The Primary Cilium as a Novel Extracellular Sensor in Bone
Hoey, D. A., Chen, J. C., & Jacobs, C. R. (2012) · Frontiers in Endocrinology · doi:10.3389/fendo.2012.00075 · Cited by 59
Self-citation
Mechanobiology of limb musculoskeletal development
Arvind, V., & Huang, A. H. (2017) · Annals of the New York Academy of Sciences · doi:10.1111/nyas.13427 · Cited by 60
The Mechanotransduction Signaling Pathways in the Regulation of Osteogenesis
Liu, Z., Wang, Q., Zhang, J., Qi, S., Duan, Y., & Li, C. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241814326 · Cited by 77
Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole–cilium interface and facilitates proper cilium formation and function
Lee, Y. L., Sante, J. M., Comerci, C. J., Cyge, B., Menezes, L. F., Li, F., Germino, G. G., Moerner, W. E., Takemaru, K., & Stearns, T. (2014) · Molecular Biology of the Cell · doi:10.1091/mbc.e14-02-0735 · Cited by 64
Self-citation
Osteocyte-Mediated Translation of Mechanical Stimuli to Cellular Signaling and Its Role in Bone and Non-bone-Related Clinical Complications
Yan, Y., Wang, L., Ge, L., & Pathak, J. L. (2020) · Current Osteoporosis Reports · doi:10.1007/s11914-020-00564-9 · Cited by 63
The role of centrosomes in fertilization, cell division and establishment of asymmetry during embryo development
Schatten, H., & Sun, Q. (2010) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2010.01.012 · Cited by 57
Mechanobiology of bone
Iolascon, G., Resmini, G., & Tarantino, U. (2013) · Aging Clinical and Experimental Research · doi:10.1007/s40520-013-0101-2 · Cited by 58
High-resolution 3D imaging of osteocytes and computational modelling in mechanobiology: insights on bone development, ageing, health and disease
Goggin, P., Zygalakis, K. C., Oreffo, R. O. C., & Schneider, P. (2016) · European Cells and Materials · doi:10.22203/ecm.v031a18 · Cited by 56
Origin of Reparative Stem Cells in Fracture Healing
Bragdon, B. C., & Bahney, C. S. (2018) · Current Osteoporosis Reports · doi:10.1007/s11914-018-0458-4 · Cited by 57
Osteoblastic differentiation enhances expression of TRPV4 that is required for calcium oscillation induced by mechanical force
Suzuki, T., Notomi, T., Miyajima, D., Mizoguchi, F., Hayata, T., Nakamoto, T., Hanyu, R., Kamolratanakul, P., Mizuno, A., Suzuki, M., et al. (2013) · Bone · doi:10.1016/j.bone.2013.01.001 · Cited by 56
The cytoskeleton and connected elements in bone cell mechano-transduction
Gould, N. R., Torre, O. M., Leser, J. M., & Stains, J. P. (2021) · Bone · doi:10.1016/j.bone.2021.115971 · Cited by 56
Role of Intraflagellar Transport and Primary Cilia in Skeletal Development
Serra, R. A. (2008) · The Anatomical Record · doi:10.1002/ar.20634 · Cited by 52
Estrogen deficiency attenuates fluid flow‐induced [Ca 2+ ] i oscillations and mechanoresponsiveness of MLO‐Y4 osteocytes
Deepak, V., Kayastha, P., & McNamara, L. M. (2017) · The FASEB Journal · doi:10.1096/fj.201601280r · Cited by 57
Physiological mechanisms and therapeutic potential of bone mechanosensing
Xiao, Z., & Quarles, L. D. (2015) · Reviews in Endocrine and Metabolic Disorders · doi:10.1007/s11154-015-9313-4 · Cited by 56
Mechanics rules cell biology
Wang, J. H., & Li, B. (2010) · BMC Sports Science Medicine and Rehabilitation · doi:10.1186/1758-2555-2-16 · Cited by 52
FOP Is a Centriolar Satellite Protein Involved in Ciliogenesis
Lee, J. Y., & Stearns, T. (2013) · PLoS ONE · doi:10.1371/journal.pone.0058589 · Cited by 55
Self-citation
Insights into interstitial flow, shear stress, and mass transport effects on ECM heterogeneity in bioreactor-cultivated engineered cartilage hydrogels
Chen, T., Buckley, M. R., Cohen, I., et al. (2011) · Biomechanics and Modeling in Mechanobiology · doi:10.1007/s10237-011-0343-x · Cited by 52
The Subchondral Bone Is Affected by Bone Marrow Stimulation: A Systematic Review of Preclinical Animal Studies
Seow, D., Yasui, Y., Hutchinson, I. D., et al. (2017) · Cartilage · doi:10.1177/1947603517711220 · Cited by 52
SLUG Is Expressed in Endothelial Cells Lacking Primary Cilia to Promote Cellular Calcification
Sánchez‐Duffhues, G., de Vinuesa, A. G., Lindeman, J. H. N., Mulder-Stapel, A. A., DeRuiter, M. C., van Munsteren, C., Goumans, M., Hierck, B. P., & ten Dijke, P. (2015) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.115.305268 · Cited by 51
A paradigm shift for bone quality in dentistry: A literature review
Kuroshima, S., Kaku, M., Ishimoto, T., Sasaki, M., Nakano, T., & Sawase, T. (2017) · Journal of Prosthodontic Research · doi:10.1016/j.jpor.2017.05.006 · Cited by 53
Identification of Elongated Primary Cilia with Impaired Mechanotransduction in Idiopathic Scoliosis Patients
Oliazadeh, N., Gorman, K. F., Eveleigh, R. J. M., Bourque, G., & Moreau, A. (2017) · Scientific Reports · doi:10.1038/srep44260 · Cited by 51
A Trabecular Bone Explant Model of Osteocyte–Osteoblast Co-Culture for Bone Mechanobiology
Chan, M. E., Lu, X. L., Huo, B., Baik, A. D., Chiang, V., Guldberg, R. E., Lu, H. H., & Guo, X.-D. E. (2009) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-009-0075-5 · Cited by 51
Polycystin-1 Mediates Mechanical Strain-Induced Osteoblastic Mechanoresponses via Potentiation of Intracellular Calcium and Akt/β-Catenin Pathway
Wang, H., Sun, W. S., Ma, J., Pan, Y., Wang, L., & Zhang, W. (2014) · PLoS ONE · doi:10.1371/journal.pone.0091730 · Cited by 51
Flow-induced mechanotransduction in skeletal cells
Alfieri, R. R., Vassalli, M., & Viti, F. (2019) · Biophysical Reviews · doi:10.1007/s12551-019-00596-1 · Cited by 51
Bone fracture healing: perspectives according to molecular basis
Ruggieri, I. N. C., Cícero, A. M., Issa, J. P. M., & Feldman, S. (2020) · Journal of Bone and Mineral Metabolism · doi:10.1007/s00774-020-01168-0 · Cited by 52
The importance of foetal movement for co-ordinated cartilage and bone development in utero
Shea, C. A., Rolfe, R. A., & Murphy, P. (2015) · Bone and Joint Research · doi:10.1302/2046-3758.47.2000387 · Cited by 49
Primary cilia as the nexus of biophysical and hedgehog signaling at the tendon enthesis
Fang, F., Schwartz, A. G., Moore, E. R., Sup, M. E., & Thomopoulos, S. (2020) · Science Advances · doi:10.1126/sciadv.abc1799 · Cited by 48
An experimental and computational analysis of primary cilia deflection under fluid flow
Downs, M. E., Nguyen, A. M., Herzog, F. A., Hoey, D. A., & Jacobs, C. R. (2012) · Computer Methods in Biomechanics & Biomedical Engineering · doi:10.1080/10255842.2011.653784 · Cited by 47
Self-citation
Application of computational fluid dynamics in tissue engineering
Patrachari, A. R., Podichetty, J. T., & Madihally, S. V. (2012) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2012.03.010 · Cited by 49
Centrosome Fine Ultrastructure of the Osteocyte Mechanosensitive Primary Cilium
Uzbekov, R. E., Maurel, D. B., Aveline, P. C., Pallu, S., Benhamou, C. L., & Rochefort, G. Y. (2012) · Microscopy and Microanalysis · doi:10.1017/s1431927612013281 · Cited by 51
Primary Cilia: The Chemical Antenna Regulating Human Adipose-Derived Stem Cell Osteogenesis
Bodle, J. C., Rubenstein, C. D., Phillips, M. E., Bernacki, S. H., Qi, J., Banes, A. J., & Loboa, E. G. (2013) · PLoS ONE · doi:10.1371/journal.pone.0062554 · Cited by 46
Stimulation of Bone Repair with Ultrasound
Padilla, F. R., Puts, R., Vico, L., Guignandon, A., & Raum, K. (2015) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-22536-4_21 · Cited by 48
Pulsed electromagnetic fields regulate osteocyte apoptosis, RANKL/OPG expression, and its control of osteoclastogenesis depending on the presence of primary cilia
Wang, P., Tang, C., Wu, J., Yang, Y., Yan, Z., Liu, X., Shao, X., Zhai, M., Gao, J., Liang, S., et al. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.27734 · Cited by 46
Concise Review: Primary Cilia: Control Centers for Stem Cell Lineage Specification and Potential Targets for Cell-Based Therapies
Bodle, J. C., & Loboa, E. G. (2016) · Stem Cells · doi:10.1002/stem.2341 · Cited by 46
Zic1 transcription factor in bone: neural developmental protein regulates mechanotransduction in osteocytes
Kalogeropoulos, M., Varanasi, S. S., Olstad, O. K., Sanderson, P. L., Gautvik, V. T., Reppe, S., Francis, R. M., Gautvik, K. M., Birch, M., & Datta, H. K. (2010) · The FASEB Journal · doi:10.1096/fj.09-148908 · Cited by 45
Primary Cilia and Calcium Signaling Interactions
Saternos, H. C., Ley, S. T., & AbouAlaiwi, W. A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21197109 · Cited by 46
Tunnels in the rock: Dynamics of osteocyte morphogenesis
Moharrer, Y., & Boerckel, J. D. (2021) · Bone · doi:10.1016/j.bone.2021.116104 · Cited by 43
Pathophysiological mechanism of acute bone loss after fracture
Zheng, X.-Q., Huang, J., Lin, J., & Song, C. (2022) · Journal of Advanced Research · doi:10.1016/j.jare.2022.08.019 · Cited by 47
A fluid–structure interaction model to characterize bone cell stimulation in parallel-plate flow chamber systems
Vaughan, T. J., Haugh, M. G., & McNamara, L. M. (2013) · Journal of The Royal Society Interface · doi:10.1098/rsif.2012.0900 · Cited by 43
Mechanical loading disrupts osteocyte plasma membranes which initiates mechanosensation events in bone
Yu, K., Sellman, D., Bahraini, A., Hagan, M. L., Elsherbini, A. M., Vanpelt, K. T., Marshall, P. L., Hamrick, M. W., McNeil, A. K., McNeil, P. L., et al. (2017) · Journal of Orthopaedic Research® · doi:10.1002/jor.23665 · Cited by 43
The effects of locomotion on bone marrow mesenchymal stem cell fate: insight into mechanical regulation and bone formation
Sun, Y., Yuan, Y. J., Wu, W., Lei, L., & Zhang, L. (2021) · Cell & Bioscience · doi:10.1186/s13578-021-00601-9 · Cited by 43
Primary cilia mechanics affects cell mechanosensation: A computational study
Khayyeri, H., Barreto, S., & Lacroix, D. (2015) · Journal of Theoretical Biology · doi:10.1016/j.jtbi.2015.04.034 · Cited by 42
Direct demonstration of tubular fluid flow sensing by macula densa cells
Sipos, A., Vargas, S. L., & Peti‐Peterdi, J. (2010) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00469.2009 · Cited by 42
Calcium channels in primary cilia
Nauli, S. M., Pala, R., & Kleene, S. J. (2016) · Current Opinion in Nephrology & Hypertension · doi:10.1097/mnh.0000000000000251 · Cited by 42
Low‐intensity pulsed ultrasound stimulation promotes osteoblast differentiation through hedgehog signaling
Matsumoto, K., Shimo, T., Kurio, N., Okui, T., Ibaragi, S., Kunisada, Y., Obata, K., Masui, M., Pang, P., Horikiri, Y., et al. (2017) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26418 · Cited by 42
The Interaction of Biological Factors with Mechanical Signals in Bone Adaptation: Recent Developments
Robling, A. G. (2012) · Current Osteoporosis Reports · doi:10.1007/s11914-012-0099-y · Cited by 41
Primary Cilia Exist in a Small Fraction of Cells in Trabecular Bone and Marrow
Coughlin, T. R., Voisin, M. C., Schaffler, M. B., Niebur, G. L., & McNamara, L. M. (2014) · Calcified Tissue International · doi:10.1007/s00223-014-9928-6 · Cited by 41
Osteocyte function under compressive mechanical force
Takano‐Yamamoto, T. (2013) · Japanese Dental Science Review · doi:10.1016/j.jdsr.2013.10.004 · Cited by 46
The sclerostin-bone protein interactome
Ketha, H., Craig, T. A., Madden, B. J., Bergen, H. R., & Kumar, R. (2011) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2011.12.048 · Cited by 41
Mechanisms of bone response to injury
Dittmer, K. E., & Firth, E. C. (2017) · Journal of Veterinary Diagnostic Investigation · doi:10.1177/1040638716679861 · Cited by 41
Shear-mediated crystallization from amorphous calcium phosphate to bone apatite
Niu, X., Wang, L., Tian, F., Wang, L., Li, P., Feng, Q., & Fan, Y. (2015) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2015.09.024 · Cited by 41
Ciliary dysfunction in polycystic kidney disease: an emerging model with polarizing potential
Robert, J. K. (2008) · Frontiers in bioscience · doi:10.2741/3016 · Cited by 41
Convergence of Calcium Channel Regulation and Mechanotransduction in Skeletal Regenerative Biomaterial Design
LaGuardia, J. S., Shariati, K., Bedar, M., Ren, X., Moghadam, S., Huang, K. X., Chen, W., Kang, Y., Yamaguchi, D. T., & Lee, J. C. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202301081 · Cited by 43
Primary cilia are necessary for Prx1-expressing cells to contribute to postnatal skeletogenesis
Moore, E. R., Yang, Y., & Jacobs, C. R. (2018) · Journal of Cell Science · doi:10.1242/jcs.217828 · Cited by 40
Self-citation
The growth plate’s response to load is partially mediated by mechano-sensing via the chondrocytic primary cilium
Rais, Y., Reich, A., Simsa-Maziel, S., Moshe, M., Idelevich, A., Kfir, T., Miosge, N., & Ornan, E. M. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1690-4 · Cited by 38
In situ deflection of tendon cell‐cilia in response to tensile loading: an in vitro study
Lavagnino, M., ARNOCZKY, S. P., & Gardner, K. L. (2011) · Journal of Orthopaedic Research® · doi:10.1002/jor.21337 · Cited by 39
Interleukin-1β sequesters hypoxia inducible factor 2α to the primary cilium
Wann, A. K., Thompson, C. L., Chapple, J. P., & Knight, M. M. (2013) · Cilia · doi:10.1186/2046-2530-2-17 · Cited by 38
Axonemal Lumen Dominates Cytosolic Protein Diffusion inside the Primary Cilium
Luo, W., Ruba, A., Takao, D., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-16103-z · Cited by 38
Negative Regulation of Hedgehog Signaling by Liver X Receptors
Kim, W.-K., Meliton, V., Park, K. W., Hong, C., Tontonoz, P., Niewiadomski, P., Waschek, J. A., Tetradis, S., & Parhami, F. (2009) · Molecular Endocrinology · doi:10.1210/me.2008-0453 · Cited by 39
Biological and Chemical Removal of Primary Cilia Affects Mechanical Activation of Chondrogenesis Markers in Chondroprogenitors and Hypertrophic Chondrocytes
Deren, M. E., Yang, X., Guan, Y. A., & Chen, Q. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17020188 · Cited by 38
Resveratrol protects primary cilia integrity of human mesenchymal stem cells from cigarette smoke to improve osteogenic differentiation in vitro
Sreekumar, V., Aspera-Werz, R. H., Ehnert, S., Strobel, J., Tendulkar, G., Heid, D., Schreiner, A. J., Arnscheidt, C., & Nussler, A. K. (2017) · Archives of Toxicology · doi:10.1007/s00204-017-2149-9 · Cited by 38
Multiscale Modeling Provides Differentiated Insights to Fluid Flow-Driven Stimulation of Bone Cellular Activities
Estermann, S.-J., & Scheiner, S. (2018) · Frontiers in Physics · doi:10.3389/fphy.2018.00076 · Cited by 37
Adenylyl cyclase 6 mediates loading‐induced bone adaptation in vivo
Lee, K. L., Hoey, D. A., Spasić, M. R., Tang, T., Hammond, H. K., & Jacobs, C. R. (2013) · The FASEB Journal · doi:10.1096/fj.13-240432 · Cited by 37
Self-citation
Are all osteocytes equal? Multiscale modelling of cortical bone to characterise the mechanical stimulation of osteocytes
Vaughan, T. J., Verbruggen, S. W., & McNamara, L. M. (2013) · International Journal for Numerical Methods in Biomedical Engineering · doi:10.1002/cnm.2578 · Cited by 37
Sensing in the Mouth: A Model for Filiform Papillae as Strain Amplifiers
Lauga, E., Pipe, C. J., & Révérend, B. L. (2016) · Frontiers in Physics · doi:10.3389/fphy.2016.00035 · Cited by 37
PTH1R translocation to primary cilia in mechanically‐stimulated ostecytes prevents osteoclast formation via regulation of CXCL5 and IL‐6 secretion
Tirado‐Cabrera, I., Martín‐Guerrero, E., Heredero‐Jiménez, S., Ardura, J. A., & Gortázar, A. R. (2022) · Journal of Cellular Physiology · doi:10.1002/jcp.30849 · Cited by 36
Identification and functional characterization of the Piezo1 channel pore domain
Nosyreva, E. D., Thompson, D., & Syeda, R. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.ra120.015905 · Cited by 35
Primary cilia act as mechanosensors during bone healing around an implant
Leucht, P., Monica, S. D., Temiyasathit, S., Lenton, K. A., Manu, A., Longaker, M. T., Jacobs, C. R., Spilker, R. L., Guo, H., Brunski, J., et al. (2012) · Medical Engineering & Physics · doi:10.1016/j.medengphy.2012.06.005 · Cited by 37
Self-citation
The structure-mechanics relationship and the response to load of the acellular bone of neoteleost fish: a review
Dean, M. N., & Shahar, R. (2012) · Journal of Applied Ichthyology · doi:10.1111/j.1439-0426.2012.01991.x · Cited by 36
The dentin–pulp border: a dynamic interface between hard and soft tissues
Tjäderhane, L., & Haapasalo, M. (2009) · Endodontic Topics · doi:10.1111/j.1601-1546.2012.00266.x · Cited by 36
Cilia Ift protein and motor -related bone diseases and mouse models
Yang, S. (2015) · Frontiers in bioscience · doi:10.2741/4323 · Cited by 37
Mechanisms of fluid-flow-induced matrix production in bone tissue engineering
Morris, H. L., Reed, C. I., Haycock, J. W., et al. (2010) · Proceedings of the Institution of Mechanical Engineers Part H Journal of Engineering in Medicine · doi:10.1243/09544119jeim751 · Cited by 35
Immersed Boundary Models for Quantifying Flow-Induced Mechanical Stimuli on Stem Cells Seeded on 3D Scaffolds in Perfusion Bioreactors
Guyot, Y., Smeets, B., Odenthal, T., Subramani, R., Luyten, F. P., Ramón, H., Papantoniou, I., & Geris, L. (2016) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1005108 · Cited by 34
Mesenchymal stem cell mechanotransduction is cAMP dependent and regulated by adenylyl cyclase 6 and the primary cilium
Johnson, G. P., Stavenschi, E., Eichholz, K. F., Corrigan, M. A., Fair, S., & Hoey, D. A. (2018) · Journal of Cell Science · doi:10.1242/jcs.222737 · Cited by 36
UTP-induced ATP release is a fine-tuned signalling pathway in osteocytes
Kringelbach, T. M., Aslan, D., Novak, I., Schwarz, P., & Jørgensen, N. R. (2014) · Purinergic Signalling · doi:10.1007/s11302-013-9404-1 · Cited by 34
Primary cilia act as microgravity sensors by depolymerizing microtubules to inhibit osteoblastic differentiation and mineralization
Shi, W., Zhang, Y., Chen, K., et al. (2020) · Bone · doi:10.1016/j.bone.2020.115346 · Cited by 34
Mechanotransduction
Tschumperlin, D. J. (2011) · Comprehensive physiology · doi:10.1002/cphy.c100016 · Cited by 46
Fluid flow stress induced contraction and re-spread of mesenchymal stem cells: a microfluidic study
Zheng, W., Xie, Y., Zhang, W., Wang, D., Ma, W., Wang, Z., & Jiang, X. (2012) · Integrative Biology · doi:10.1039/c2ib20094e · Cited by 33
Cell Signaling in Tenocytes: Response to Load and Ligands in Health and Disease
Wall, M. E., Dyment, N. A., Bodle, J. C., et al. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-33943-6_7 · Cited by 33

Authors: Malone, A. M. D., Anderson, C. T., Tummala, P., Kwon, R. Y., Johnston, T. R., Stearns, T., & Jacobs, C. R.
Affiliations: VA Palo Alto Health Care System (US)
Stanford University (US)
Stanford Medicine (US)
ORCID: Anderson, Charles T.
Tummala, Padmaja
Kwon, Ronald Y.
Johnston, Tyler R.
Stearns, Tim
Jacobs, Christopher R.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2007-08-14
Volume: 104
Issue: 33
Pages: 13325-13330
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.0700636104
PMC: PMC1939687
OpenAlex: W2104440688
Semantic Scholar: f451262cfba816230b82b3b56064b900c1cc691a
Cited by: 477
References: 102
Citations by year: 2026: 17 · 2025: 11 · 2024: 9 · 2023: 20 · 2022: 21 · 2021: 24 · 2020: 32 · 2019: 21 · 2018: 19 · 2017: 26 · 2016: 33 · 2015: 27 · 2014: 24 · 2013: 36 · 2012: 40
FWCI: 15.53
Type: journal article
Language: en
Update notices: correction (2008-01-15)
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: University of Missouri
National Institutes of Health
National Institute of General Medical Sciences (R01GM052022)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR-45989, R01AR045989)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords: primary cilia, mechanosensing, mechanotransduction, bone cells, fluid flow, osteogenic differentiation, bone resorption, calcium-independent signaling, stretch-activated channels, bone homeostasis, osteoporosis, disuse bone loss
MeSH terms: Animals, Base Sequence, Calcium, Cilia, Fluorescent Antibody Technique, Osteoblasts, Blotting, Western, 3T3 Cells, DNA Primers, Mechanotransduction, Cellular, TRPP Cation Channels, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Primary cilia mediate mechanosensing in bone cells by a calcium-independent mechanism

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks