The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Physical plasticity of the nucleus in stem cell differentiation
Open accessAbstract
Cell differentiation in embryogenesis involves extensive changes in gene expression structural reorganization within the nucleus, including chromatin condensation and nucleoprotein immobilization. We hypothesized that nuclei in naive stem cells would therefore prove to be physically plastic and also more pliable than nuclei in differentiated cells. Micromanipulation methods indeed show that nuclei in human embryonic stem cells are highly deformable and stiffen 6-fold through terminal differentiation, and that nuclei in human adult stem cells possess an intermediate stiffness and deform irreversibly. Because the nucleo-skeletal component Lamin A/C is not expressed in either type of stem cell, we knocked down Lamin A/C in human epithelial cells and measured a deformability similar to that of adult hematopoietic stem cells. Rheologically, lamin-deficient states prove to be the most fluid-like, especially within the first ≈10 sec of deformation. Nuclear distortions that persist longer than this are irreversible, and fluorescence-imaged microdeformation with photobleaching confirms that chromatin indeed flows, distends, and reorganizes while the lamina stretches. The rheological character of the nucleus is thus set largely by nucleoplasm/chromatin, whereas the extent of deformation is modulated by the lamina.
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Cites
Calcium's Role in Mechanotransduction during Muscle Development
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Larson, R. G. (1998) · Cited by 3,188
Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
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Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction
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Cited by
Growth Factors, Matrices, and Forces Combine and Control Stem Cells
Discher, D. E., Mooney, D. J., & Zandstra, P. W. (2009) · Science · doi:10.1126/science.1171643 · Cited by 2,667
Self-citationMechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus
Wang, N., Tytell, J. D., & Ingber, D. E. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2594 · Cited by 1,762
Materials as stem cell regulators
Murphy, W. L., McDevitt, T. C., & Engler, A. J. (2014) · Nature Materials · doi:10.1038/nmat3937 · Cited by 947
Dechat, T., Pfleghaar, K., Sengupta, K., Shimi, T., Shumaker, D. K., Solimando, L., & Goldman, R. D. (2008) · Genes & Development · doi:10.1101/gad.1652708 · Cited by 940
Isolated nuclei adapt to force and reveal a mechanotransduction pathway in the nucleus
Guilluy, C., Osborne, L. D., Van Landeghem, L., Sharek, L., Superfine, R., Garcia-Mata, R., & Burridge, K. (2014) · Nature Cell Biology · doi:10.1038/ncb2927 · Cited by 607
Metadata
- Authors
- Pajerowski, J. D., Dahl, K. N., Zhong, F. L., Sammak, P. J., & Discher, D. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2007-10-02
- Volume
- 104
- Issue
- 40
- Pages
- 15619-15624
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2172002964
- Cited by
- 833
- References
- 49
- FWCI
- 9.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Pajerowski, J. D., Dahl, K. N., Zhong, F. L., Sammak, P. J., & Discher, D. E. (2007). Physical plasticity of the nucleus in stem cell differentiation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0702576104
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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