The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Hyperpolarized 13 C allows a direct measure of flux through a single enzyme-catalyzed step by NMR
Open accessAbstract
13 C NMR is a powerful tool for monitoring metabolic fluxes in vivo . The recent availability of automated dynamic nuclear polarization equipment for hyperpolarizing 13 C nuclei now offers the potential to measure metabolic fluxes through select enzyme-catalyzed steps with substantially improved sensitivity. Here, we investigated the metabolism of hyperpolarized [1- 13 C 1 ]pyruvate in a widely used model for physiology and pharmacology, the perfused rat heart. Dissolved 13 CO 2 , the immediate product of the first step of the reaction catalyzed by pyruvate dehydrogenase, was observed with a temporal resolution of ≈1 s along with H 13 CO 3 − , the hydrated form of 13 CO 2 generated catalytically by carbonic anhydrase. In hearts presented with the medium-chain fatty acid octanoate in addition to hyperpolarized [1- 13 C 1 ]pyruvate, production of 13 CO 2 and H 13 CO 3 − was suppressed by ≈90%, whereas the signal from [1- 13 C 1 ]lactate was enhanced. In separate experiments, it was shown that O 2 consumption and tricarboxylic acid (TCA) cycle flux were unchanged in the presence of added octanoate. Thus, the rate of appearance of 13 CO 2 and H 13 CO 3 − from [1- 13 C 1 ]pyruvate does not reflect production of CO 2 in the TCA cycle but rather reflects flux through pyruvate dehydrogenase exclusively.
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Increase in signal-to-noise ratio of > 10,000 times in liquid-state NMR
Ardenkjær-Larsen, J. H., Fridlund, B., Gram, A., Hansson, G., Hansson, L., Lerche, M. H., Servin, R., Thaning, M., & Golman, K. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1733835100 · Cited by 2,861
The Failing Heart — An Engine Out of Fuel
Neubauer, S. (2007) · New England Journal of Medicine · doi:10.1056/nejmra063052 · Cited by 2,417
Golman, K., in ‘t Zandt, R., & Thaning, M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601319103 · Cited by 659
The CREATE-ECLA Trial Group Investigators* (2005) · JAMA · doi:10.1001/jama.293.4.437 · Cited by 641
Chaitman, B. R., Skettino, S. L., Parker, J. O., Hanley, P., Meluzin, J., Kuch, J., Pepine, C. J., Wang, W., Nelson, J. J., Hebert, D. A., et al. (2004) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2003.11.045 · Cited by 567
Sodi-Pallares, D., Testelli, M. R., Fishleder, B. L., Bisteni, A., Medrano, G. A., Friedland, C., & Micheli, A. D. (1962) · The American Journal of Cardiology · doi:10.1016/0002-9149(62)90035-8 · Cited by 503
Studies of acidosis in the ischaemic heart by phosphorus nuclear magnetic resonance
Garlick, P. B., Radda, G. K., & Seeley, P. J. (1979) · Biochemical Journal · doi:10.1042/bj1840547 · Cited by 483
Molecular imaging with endogenous substances
Golman, K., Ardenkjær-Larsen, J. H., Petersson, J. S., Månsson, S., & Leunbach, I. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1733836100 · Cited by 427
Lee, L., Campbell, R., Scheuermann-Freestone, M., Taylor, R., Gunaruwan, P., Williams, L., Ashrafian, H., Horowitz, J., Fraser, A. G., Clarke, K., et al. (2005) · Circulation · doi:10.1161/circulationaha.105.551457 · Cited by 357
Malloy, C. R., Sherry, A. D., & Jeffrey, F. M. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68590-4 · Cited by 322
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Cited by
Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate
Gallagher, F. A., Kettunen, M. I., Day, S. E., Hu, D.-E., Ardenkjær-Larsen, J. H., Zandt, R. I. ‘., Jensen, P. R., Karlsson, M., Golman, K., Lerche, M. H., et al. (2008) · Nature · doi:10.1038/nature07017 · Cited by 849
Kurhanewicz, J., Vigneron, D. B., Brindle, K., Chekmenev, E. Y., Comment, A., Cunningham, C. H., DeBerardinis, R. J., Green, G. G., Leach, M. O., Rajan, S. S., et al. (2011) · Neoplasia · doi:10.1593/neo.101102 · Cited by 681
Self-citationMcGarrah, R. W., Crown, S. B., Zhang, G.-F., Shah, S. H., & Newgard, C. B. (2018) · Circulation Research · doi:10.1161/circresaha.117.311002 · Cited by 423
Small animal cardiovascular MR imaging and spectroscopy
Bakermans, A. J., Abdurrachim, D., Moonen, R. P. M., Motaal, A. G., Prompers, J. J., Strijkers, G. J., Vandoorne, K., & Nicolay, K. (2015) · Progress in Nuclear Magnetic Resonance Spectroscopy · doi:10.1016/j.pnmrs.2015.03.001 · Cited by 35
Graham, J. E., Niks, D., Zane, G. M., Gui, Q., Hom, K., Hille, R., Wall, J. D., & Raman, C. S. (2022) · ACS Catalysis · doi:10.1021/acscatal.2c00316 · Cited by 21
Metadata
- Authors
- Merritt, M. E., Harrison, C., Storey, C., Jeffrey, F. M., Sherry, A. D., & Malloy, C. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2007-12-11
- Volume
- 104
- Issue
- 50
- Pages
- 19773-19777
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2004311009
- Cited by
- 278
- References
- 27
- FWCI
- 7.55
- Type
- journal article
- Language
- en
- Open access
- green
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