The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
A genetically encoded fluorescent sensor of ERK activity
Open accessAbstract
The activity of the ERK has complex spatial and temporal dynamics that are important for the specificity of downstream effects. However, current biochemical techniques do not allow for the measurement of ERK signaling with fine spatiotemporal resolution. We developed a genetically encoded, FRET-based sensor of ERK activity (the extracellular signal-regulated kinase activity reporter, EKAR), optimized for signal-to-noise ratio and fluorescence lifetime imaging. EKAR selectively and reversibly reported ERK activation in HEK293 cells after epidermal growth factor stimulation. EKAR signals were correlated with ERK phosphorylation, required ERK activity, and did not report the activities of JNK or p38. EKAR reported ERK activation in the dendrites and nucleus of hippocampal pyramidal neurons in brain slices after theta-burst stimuli or trains of back-propagating action potentials. EKAR therefore permits the measurement of spatiotemporal ERK signaling dynamics in living cells, including in neuronal compartments in intact tissues.
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Sun, H., Charles, C. H., Lau, L. F., & Tonks, N. K. (1993) · Cell · doi:10.1016/0092-8674(93)90383-2 · Cited by 1,156
Locally dynamic synaptic learning rules in pyramidal neuron dendrites
Harvey, C. D., & Svoboda, K. (2007) · Nature · doi:10.1038/nature06416 · Cited by 625
Self-citationJacobs, D., Glossip, D., Xing, H., Muslin, A. J., & Kornfeld, K. (1999) · Genes & Development · doi:10.1101/gad.13.2.163 · Cited by 439
Lu, P.-J., Zhou, X. Z., Liou, Y.-C., Noel, J. P., & Lu, K. P. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c100228200 · Cited by 244
Cited by
Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues
Chudakov, D. M., Matz, M. V., Lukyanov, S., & Lukyanov, K. A. (2010) · Physiological Reviews · doi:10.1152/physrev.00038.2009 · Cited by 1,375
Improving FRET dynamic range with bright green and red fluorescent proteins
Lam, A. J., St-Pierre, F., Gong, Y., Marshall, J. D., Cranfill, P. J., Baird, M. A., McKeown, M. R., Wiedenmann, J., Davidson, M. W., Schnitzer, M. J., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2171 · Cited by 732
Hirata, E., Girotti, M. R., Viros, A., Hooper, S., Spencer-Dene, B., Matsuda, M., Larkin, J., Marais, R., & Sahai, E. (2015) · Cancer Cell · doi:10.1016/j.ccell.2015.03.008 · Cited by 593
Development of an optimized backbone of FRET biosensors for kinases and GTPases
Komatsu, N., Aoki, K., Yamada, M., Yukinaga, H., Fujita, Y., Kamioka, Y., & Matsuda, M. (2011) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-01-0072 · Cited by 577
Imaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor
Tantama, M., Hung, Y. P., & Yellen, G. (2011) · Journal of the American Chemical Society · doi:10.1021/ja202902d · Cited by 408
Metadata
- Authors
- Harvey, C. D., Ehrhardt, A. G., Cellurale, C., Zhong, H., Yasuda, R., Davis, R. J., & Svoboda, K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-12-09
- Volume
- 105
- Issue
- 49
- Pages
- 19264-19269
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2040172334
- Cited by
- 345
- References
- 4
- FWCI
- 5.71
- Type
- journal article
- Language
- en
- Open access
- green
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