The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Development of an optimized backbone of FRET biosensors for kinases and GTPases
Open accessAbstract
Biosensors based on the principle of Förster (or fluorescence) resonance energy transfer (FRET) have shed new light on the spatiotemporal dynamics of signaling molecules. Among them, intramolecular FRET biosensors have been increasingly used due to their high sensitivity and user-friendliness. Time-consuming optimizations by trial and error, however, obstructed the development of intramolecular FRET biosensors. Here we report an optimized backbone for rapid development of highly sensitive intramolecular FRET biosensors. The key concept is to exclude the “orientation-dependent” FRET and to render the biosensors completely “distance-dependent” with a long, flexible linker. We optimized a pair of fluorescent proteins for distance-dependent biosensors, and then developed a long, flexible linker ranging from 116 to 244 amino acids in length, which reduced the basal FRET signal and thereby increased the gain of the FRET biosensors. Computational simulations provided insight into the mechanisms by which this optimized system was the rational strategy for intramolecular FRET biosensors. With this backbone system, we improved previously reported FRET biosensors of PKA, ERK, JNK, EGFR/Abl, Ras, and Rac1. Furthermore, this backbone enabled us to develop novel FRET biosensors for several kinases of RSK, S6K, Akt, and PKC and to perform quantitative evaluation of kinase inhibitors in living cells.
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Self-citationNuclear and cytosolic JNK signalling in neurons
Coffey, E. T. (2014) · Nature Reviews Neuroscience · doi:10.1038/nrn3729 · Cited by 338
A highly photostable and bright green fluorescent protein
Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01278-2 · Cited by 293
An odorant-binding protein required for suppression of sweet taste by bitter chemicals
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Metadata
- Authors
- Komatsu, N., Aoki, K., Yamada, M., Yukinaga, H., Fujita, Y., Kamioka, Y., & Matsuda, M.
- Venue
- Molecular Biology of the Cell
- Published
- 2011-12
- Volume
- 22
- Issue
- 23
- Pages
- 4647-4656
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2113974479
- Cited by
- 577
- References
- 41
- FWCI
- 13.88
- Type
- journal article
- Language
- en
- Open access
- green
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