The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification
Open accessAbstract
For vertebrate olfactory signal transduction, a calcium-activated chloride conductance serves as a major amplification step. However, the molecular identity of the olfactory calcium-activated chloride channel (CaCC) is unknown. Here we report a proteomic screen for cilial membrane proteins of mouse olfactory sensory neurons (OSNs) that identified all the known olfactory transduction components as well as Anoctamin 2 (ANO2). Ano2 transcripts were expressed specifically in OSNs in the olfactory epithelium, and ANO2::EGFP fusion protein localized to the OSN cilia when expressed in vivo using an adenoviral vector. Patch-clamp analysis revealed that ANO2, when expressed in HEK-293 cells, forms a CaCC and exhibits channel properties closely resembling the native olfactory CaCC. Considering these findings together, we propose that ANO2 constitutes the olfactory calcium-activated chloride channel.
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Studies on expression and function of the TMEM16A calcium-activated chloride channel
Huang, F., Rock, J. R., Harfe, B. D., Cheng, T., Huang, X., Jan, Y. N., & Jan, L. Y. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0911935106 · Cited by 310
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Ayoglu, B., Mitsios, N., Kockum, I., Khademi, M., Zandian, A., Sjöberg, R., Forsström, B., Bredenberg, J., Lima Bomfim, I., Holmgren, E., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1518553113 · Cited by 111
Deep Sequencing of the Murine Olfactory Receptor Neuron Transcriptome
Kanageswaran, N., Demond, M., Nagel, M., Schreiner, B. S. P., Baumgart, S., Scholz, P., Altmüller, J., Becker, C., Doerner, J. F., Conrad, H., et al. (2015) · PLOS ONE · doi:10.1371/journal.pone.0113170 · Cited by 85
Metadata
- Authors
- Stephan, A. B., Shum, E. Y., Hirsh, S., Cygnar, K. D., Reisert, J., & Zhao, H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2009-07-14
- Volume
- 106
- Issue
- 28
- Pages
- 11776-11781
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2032864009
- Cited by
- 342
- References
- 30
- FWCI
- 12.60
- Type
- journal article
- Language
- en
- Open access
- green
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