Deep Sequencing of the Murine Olfactory Receptor Neuron Transcriptome
Open accessAbstract
The ability of animals to sense and differentiate among thousands of odorants relies on a large set of olfactory receptors (OR) and a multitude of accessory proteins within the olfactory epithelium (OE). ORs and related signaling mechanisms have been the subject of intensive studies over the past years, but our knowledge regarding olfactory processing remains limited. The recent development of next generation sequencing (NGS) techniques encouraged us to assess the transcriptome of the murine OE. We analyzed RNA from OEs of female and male adult mice and from fluorescence-activated cell sorting (FACS)-sorted olfactory receptor neurons (ORNs) obtained from transgenic OMP-GFP mice. The Illumina RNA-Seq protocol was utilized to generate up to 86 million reads per transcriptome. In OE samples, nearly all OR and trace amine-associated receptor (TAAR) genes involved in the perception of volatile amines were detectably expressed. Other genes known to participate in olfactory signaling pathways were among the 200 genes with the highest expression levels in the OE. To identify OE-specific genes, we compared olfactory neuron expression profiles with RNA-Seq transcriptome data from different murine tissues. By analyzing different transcript classes, we detected the expression of non-olfactory GPCRs in ORNs and established an expression ranking for GPCRs detected in the OE. We also identified other previously undescribed membrane proteins as potential new players in olfaction. The quantitative and comprehensive transcriptome data provide a virtually complete catalogue of genes expressed in the OE and present a useful tool to uncover candidate genes involved in, for example, olfactory signaling, OR trafficking and recycling, and proliferation.
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The de-enrichment of Ace2 and Tmprss2 in olfactory sensory neurons relative to whole olfactory mucosa was also observed in two other mouse RNA-Seq datasets.
“The de-enrichment of Ace2 and Tmprss2 in OSNs relative to WOM was also observed in two other mouse RNA-Seq datasets (47, 48) (Figure S4B).”
From Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia (2020) · cites this paper as [47]
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- [77]Stephan AB, Shum EY, Hirsh S, Cygnar KD, Reisert J, et al (2009) ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification. Proc. Natl. Acad. Sci. U.S.A. 106 (28): 11776– 11781. 10.1073/pnas.0903304106 19561302 PMC2702256 · DOI
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Brann, D. H., Tsukahara, T., Weinreb, C., Lipovsek, M., Van den Berge, K., Gong, B., Chance, R., Macaulay, I. C., Chou, H.-J., Fletcher, R. B., et al. (2020) · Science Advances · doi:10.1126/sciadv.abc5801 · Cited by 1,198 · see citing works
Butowt, R., & Bilinska, K. (2020) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.0c00172 · Cited by 346 · see citing works
- Authors
- Kanageswaran, N., Demond, M., Nagel, M., Schreiner, B. S. P., Baumgart, S., Scholz, P., Altmüller, J., Becker, C., Doerner, J. F., Conrad, H., Oberland, S., Wetzel, C. H., Neuhaus, E. M., Hatt, H., & Gisselmann, G.
- Affiliations
- Ruhr University Bochum (DE)
- University of Duisburg-Essen (DE)
- University of Cologne (DE)
- Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103 (DE)
- Jena University Hospital (DE)
- University of Regensburg (DE)
- Corresponding author
- Kanageswaran, Ninthujah
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-01-15
- Volume
- 10
- Issue
- 1
- Pages
- e0113170
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4295871
- OpenAlex
- W2161341195
- Cited by
- 85
- References
- 205
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 1 · 2023: 8 · 2022: 9 · 2021: 8 · 2020: 11 · 2019: 6 · 2018: 10 · 2017: 6 · 2016: 9 · 2015: 8
- FWCI
- 7.17
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Kölner Universitäts PublikationsServer (Universität zu Köln) (publishedVersion)
- MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
- Dokumentenrepositorium der RUB (Ruhr University Bochum) (submittedVersion, cc-by)
- Refubium (Universitätsbibliothek der Freien Universität Berlin) (submittedVersion, cc-by)
- Universitätsbibliothek der FU Berlin Hochschulschriftenstelle u. Dokumentenserver (cc-by)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Health Sciences › Nursing › Nutrition and Dietetics › Biochemical Analysis and Sensing Techniques
- Keywords
- olfactory sensory neurons, olfactory epithelium, transcriptome, olfactory receptors, trace amine-associated receptors, G protein-coupled receptors, olfactory signaling, odorant perception, mice
- MeSH terms
- Animals, Female, Flow Cytometry, Male, Mice, Transgenic, Olfactory Mucosa, Olfactory Pathways, Signal Transduction, Olfactory Receptor Neurons, Mice, High-Throughput Nucleotide Sequencing, Transcriptome
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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