The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Genetic engineering of human stem cells for enhanced angiogenesis using biodegradable polymeric nanoparticles
Open accessAbstract
Stem cells hold great potential as cell-based therapies to promote vascularization and tissue regeneration. However, the use of stem cells alone to promote angiogenesis remains limited because of insufficient expression of angiogenic factors and low cell viability after transplantation. Here, we have developed vascular endothelial growth factor (VEGF) high-expressing, transiently modified stem cells for the purposes of promoting angiogenesis. Nonviral, biodegradable polymeric nanoparticles were developed to deliver hVEGF gene to human mesenchymal stem cells (hMSCs) and human embryonic stem cell-derived cells (hESdCs). Treated stem cells demonstrated markedly enhanced hVEGF production, cell viability, and engraftment into target tissues. S.c. implantation of scaffolds seeded with VEGF-expressing stem cells (hMSCs and hESdCs) led to 2- to 4-fold-higher vessel densities 2 weeks after implantation, compared with control cells or cells transfected with VEGF by using Lipofectamine 2000, a leading commercial reagent. Four weeks after intramuscular injection into mouse ischemic hindlimbs, genetically modified hMSCs substantially enhanced angiogenesis and limb salvage while reducing muscle degeneration and tissue fibrosis. These results indicate that stem cells engineered with biodegradable polymer nanoparticles may be therapeutic tools for vascularizing tissue constructs and treating ischemic disease.
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Cites
1 retracted
Angiogenesis by Implantation of Peripheral Blood Mononuclear Cells and Platelets Into Ischemic Limbs
Iba, O., Matsubara, H., Nozawa, Y., Fujiyama, S., Amano, K., Mori, Y., Kojima, H., & Iwasaka, T. (2002) · Circulation · doi:10.1161/01.cir.0000031332.45480.79 · Cited by 268
RetractedVascular-specific growth factors and blood vessel formation
Yancopoulos, G. D., Davis, S., Gale, N. W., Rudge, J. S., Wiegand, S. J., & Holash, J. (2000) · Nature · doi:10.1038/35025215 · Cited by 3,699
Design and development of polymers for gene delivery
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Progress and problems with the use of viral vectors for gene therapy
Thomas, C. E., Ehrhardt, A., & Kay, M. A. (2003) · Nature Reviews Genetics · doi:10.1038/nrg1066 · Cited by 2,501
Human Mesenchymal Stem Cells Differentiate to a Cardiomyocyte Phenotype in the Adult Murine Heart
Toma, C., Pittenger, M. F., Cahill, K. S., Byrne, B. J., & Kessler, P. D. (2002) · Circulation · doi:10.1161/hc0102.101442 · Cited by 2,234
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Cited by
Current Challenges of Cancer Anti-angiogenic Therapy and the Promise of Nanotherapeutics
Abdalla, A. M. E., Xiao, L., Ullah, M. W., Yu, M., Ouyang, C., & Yang, G. (2018) · Theranostics · doi:10.7150/thno.21674 · Cited by 240
Non-genetic engineering of cells for drug delivery and cell-based therapy
Wang, Q., Cheng, H., Peng, H., Zhou, H., Li, P. Y., & Langer, R. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.12.003 · Cited by 225
Self-citationKo, E., Lee, J. S., Kim, H., Yang, S. Y., Yang, D., Yang, K., Lee, J., Shin, J., Yang, H. S., Ryu, W., et al. (2018) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.7b03328 · Cited by 150
Self-citationGenetic Engineering of Mesenchymal Stem Cells and Its Application in Human Disease Therapy
Hodgkinson, C. P., Gomez, J. A., Mirotsou, M., & Dzau, V. J. (2010) · Human Gene Therapy · doi:10.1089/hum.2010.165 · Cited by 150
Standardization of Models and Methods Used to Assess Nanoparticles in Cardiovascular Applications
Fitzgerald, K. T., Holladay, C. A., McCarthy, C., Power, K. A., Pandit, A., & Gallagher, W. M. (2011) · Small · doi:10.1002/smll.201001347 · Cited by 26
Metadata
- Authors
- Yang, F., Cho, S.-W., Son, S. M., Bogatyrev, S. R., Singh, D., Green, J. J., Mei, Y., Park, S., Bhang, S. H., Kim, B.-S., Langer, R., & Anderson, D. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2009-10-05
- Volume
- 107
- Issue
- 8
- Pages
- 3317-3322
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1977255778
- Cited by
- 301
- References
- 30
- FWCI
- 9.98
- Type
- journal article
- Language
- en
- Open access
- green
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- Yang, F., Cho, S.-W., Son, S. M., Bogatyrev, S. R., Singh, D., Green, J. J., Mei, Y., Park, S., Bhang, S. H., Kim, B.-S., Langer, R., & Anderson, D. G. (2010). Genetic engineering of human stem cells for enhanced angiogenesis using biodegradable polymeric nanoparticles. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0905432106
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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