Electrospun Silk Fibroin Nanofibrous Scaffolds with Two-Stage Hydroxyapatite Functionalization for Enhancing the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells
Abstract
The development of functional scaffolds with improved osteogenic potential is important for successful bone formation and mineralization in bone tissue engineering. In this study, we developed a functional electrospun silk fibroin (SF) nanofibrous scaffold functionalized with two-stage hydroxyapatite (HAp) particles, using mussel adhesive-inspired polydopamine (PDA) chemistry. HAp particles were first incorporated into SF scaffolds during the electrospinning process, and then immobilized onto the electrospun SF nanofibrous scaffolds containing HAp via PDA-mediated adhesive chemistry. We obtained two-stage HAp-functionalized SF nanofibrous scaffolds with improved mechanical properties and capable of providing a bone-specific physiological microenvironment. The developed scaffolds were tested for their ability to enhance the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) in vitro and repair bone defect in vivo. To boost their ability for bone repair, we genetically modified hADMSCs with the transcriptional coactivator with PDZ-binding motif (TAZ) via polymer nanoparticle-mediated gene delivery. TAZ is a well-known transcriptional modulator that activates the osteogenic differentiation of mesenchymal stem cells (MSCs). Two-stage HAp-functionalized SF scaffolds significantly promoted the osteogenic differentiation of TAZ-transfected hADMSCs in vitro and enhanced mineralized bone formation in a critical-sized calvarial bone defect model. Our s…
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HA over PDA coatings could promote osteogenic differentiation for enhanced bone formation in vivo and in vitro.
“Ko et al. established a functional electrospun silk fibroin (SF) nanofibrous scaffold functionalized with two-stage HA particles, using PDA chemistry and fruitfully confirmed that HA over PDA coatings could promote osteogenic differentiation for enhanced bone formation in vivo and in vitro (Figure 4) [43].”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [43]
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“Reprinted with permission from reference [43].”
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- Authors
- Ko, E., Lee, J. S., Kim, H., Yang, S. Y., Yang, D., Yang, K., Lee, J., Shin, J., Yang, H. S., Ryu, W., & Cho, S.-W.
- Affiliations
- Yonsei University (KR)
- Dankook University (KR)
- Corresponding author
- Ryu, WonHyoung
- Cho, Seung-Woo
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-05-05
- Volume
- 10
- Issue
- 9
- Pages
- 7614-7625
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2610551227
- Cited by
- 150
- References
- 3
- Citations by year
- 2026: 7 · 2025: 14 · 2024: 14 · 2023: 11 · 2022: 18 · 2021: 22 · 2020: 20 · 2019: 26 · 2018: 18
- FWCI
- 7.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Yonsei University (2016-22-0102)
- Ministry of Science, ICT and Future Planning (2016R1A5A1004694)
- Institute for Basic Science (IBS-R026-D1)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- silk fibroin, electrospun nanofibrous scaffolds, hydroxyapatite, polydopamine, osteogenic differentiation, adipose-derived stem cells, TAZ, gene delivery, bone tissue engineering, bone defect repair, mineralization, calvarial defect
- MeSH terms
- Cell Differentiation, Fibroins, Humans, Osteogenesis, Durapatite, Tissue Engineering, Silk, Tissue Scaffolds, Nanofibers, Mesenchymal Stem Cells
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