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A muscle-specific transgenic reporter line of the sea anemone, Nematostella vectensis
Open accessAbstract
The sea anemone, Nematostella vectensis , has become an attractive new model organism for comparative genomics and evolutionary developmental biology. Over the last few years, many genes have been isolated and their expression patterns studied to gain insight into their function. More recently, functional tools have been developed to manipulate gene function; however, most of these approaches rely on microinjection and are limited to early stages of development. Transgenic lines would significantly enhance the tractability of the system. In particular, the study of gene- or tissue-specific promoters would be most useful. Here we report the stable establishment of a transgenic line using the I-SceI meganuclease system to facilitate integration into the genome. We isolated a 1.6-kb fragment of the regulatory upstream region of the Myosin Heavy Chain1 ( MyHC1 ) gene and found that the transgene is specifically expressed in the retractor and tentacle muscles of Nematostella polyps, faithfully reproducing the expression of the endogenous MyHC1 gene. This demonstrates that the 1.6-kb fragment contains all of the regulatory elements necessary to drive correct expression and suggests that retractor and tentacle muscles in Nematostella are distinct from other myoepithelial cells. The transgene is transmitted through the germline at high frequency, and G 1 transgenic polyps have only one integration site. The relatively high frequency of transgenesis, in combination with gene- or tissue-specific promoters, will foster experimental possibilities for studying in vivo gene functions in gene regulatory networks and developmental processes in the nonbilaterian sea anemone, Nematostella vectensis .
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“The first selection marker comprised the coding sequence for mCherry under the control of the myosin heavy chain1 (MHC1) promoter, which drives expression in muscle cells 16 (MHC1-mCherry).”
From TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis (2014) · cites this paper as [16]
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Metadata
- Authors
- Renfer, E., Amon-Hassenzahl, A., Steinmetz, P. R. H., & Technau, U.
- Affiliations
- University of Vienna (AT)
- Technische Universität Darmstadt (DE)
- Corresponding author
- Technau, Ulrich
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2009-12-29
- Volume
- 107
- Issue
- 1
- Pages
- 104-108
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2806778
- OpenAlex
- W2081565890
- Cited by
- 164
- References
- 20
- Citations by year
- 2026: 8 · 2025: 11 · 2024: 16 · 2023: 5 · 2022: 18 · 2021: 13 · 2020: 7 · 2019: 7 · 2018: 5 · 2017: 8 · 2016: 10 · 2015: 11 · 2014: 12 · 2013: 3 · 2012: 11
- FWCI
- 7.77
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Phaidra (Universität Wien) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Marine Invertebrate Physiology and Ecology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- Nematostella vectensis, I-SceI meganuclease, muscle-specific promoter, retractor muscle, germline transmission, transgene integration, gene regulatory networks
- MeSH terms
- Animals, Cell Differentiation, Genetic Vectors, Muscles, Promoter Regions, Genetic, Regeneration, Sea Anemones, Genes, Reporter, Gene Transfer Techniques, Myosin Heavy Chains, Protein Isoforms, Animals, Genetically Modified
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