The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases
Open accessAbstract
High-resolution imaging of molecules intrinsically involved in malignancy and metastasis would be of great value for clinical detection and staging of tumors. We now report in vivo visualization of matrix metalloproteinase activities by MRI and fluorescence of dendrimeric nanoparticles coated with activatable cell penetrating peptides (ACPPs), labeled with Cy5, gadolinium, or both. Uptake of such nanoparticles in tumors is 4- to 15-fold higher than for unconjugated ACPPs. With fluorescent molecules, we are able to detect residual tumor and metastases as small as 200 μm, which can be resected under fluorescence guidance and analyzed histopathologically with fluorescence microscopy. We show that uptake via this mechanism is comparable to that of other near infrared protease sensors, with the added advantage that the approach is translatable to MRI. Once activated, the Gd-labeled nanoparticles deposit high levels (30–50 μM) of Gd in tumor parenchyma with even higher amounts deposited in regions of infiltrative tumor, resulting in useful T 1 contrast lasting several days after injection. These results should improve MRI-guided clinical staging, presurgical planning, and intraoperative fluorescence-guided surgery. The approach may be generalizable to deliver radiation-sensitizing and chemotherapeutic agents.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Tumor imaging by means of proteolytic activation of cell-penetrating peptides
Jiang, T., Olson, E. S., Nguyen, Q. T., Roy, M., Jennings, P. A., & Tsien, R. Y. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408191101 · Cited by 822
Self-citationNguyen, Q. T., Olson, E. S., Aguilera, T. A., Jiang, T., Scadeng, M., Ellies, L. G., & Tsien, R. Y. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0910261107 · Cited by 474
Self-citationTumor Paint: A Chlorotoxin:Cy5.5 Bioconjugate for Intraoperative Visualization of Cancer Foci
Veiseh, M., Gabikian, P., Bahrami, S.-B., Veiseh, O., Zhang, M., Hackman, R. C., Ravanpay, A. C., Stroud, M. R., Kusuma, Y., Hansen, S. J., et al. (2007) · Cancer Research · doi:10.1158/0008-5472.can-06-3948 · Cited by 422
Dendrimer-Based Nanoprobe for Dual Modality Magnetic Resonance and Fluorescence Imaging
Talanov, V. S., Regino, C. A. S., Kobayashi, H., Bernardo, M., Choyke, P. L., & Brechbiel, M. W. (2006) · Nano Letters · doi:10.1021/nl060765q · Cited by 266
Olson, E. S., Aguilera, T. A., Jiang, T., Ellies, L. G., Nguyen, Q. T., Wong, E. H., Gross, L. A., & Tsien, R. Y. (2009) · Integrative Biology · doi:10.1039/b904890a · Cited by 263
Self-citationTransgenic Polyoma middle-T mice model premalignant mammary disease.
Maglione, J. E., Moghanaki, D. M., Young, L. J. T., Manner, C. K., Ellies, L. G., Joseph, S. O., Nicholson, B., Cardiff, R. D., & MacLeod, C. L. (2001) · PubMed · Cited by 256
Self-citationOptical Imaging of Matrix Metalloproteinase–2 Activity in Tumors: Feasibility Study in a Mouse Model
Bremer, C., Bredow, S., Mahmood, U., Weissleder, R., & Tung, C.-H. (2001) · Radiology · doi:10.1148/radiol.2212010368 · Cited by 255
Inversion recovery TrueFISP: Quantification of T1, T2, and spin density
Schmitt, P., Griswold, M. A., Jakob, P. M., Kotas, M., Gulani, V., Flentje, M., & Haase, A. (2004) · Magnetic Resonance in Medicine · doi:10.1002/mrm.20058 · Cited by 242
Bryant, L. H., Brechbiel, M. W., Wu, C., Bulte, J. W. M., Herynek, V., & Frank, J. A. (1999) · Journal of Magnetic Resonance Imaging · doi:10.1002/(sici)1522-2586(199902)9:2<348::aid-jmri30>3.0.co;2-j · Cited by 236
Aguilera, T. A., Olson, E. S., Timmers, M. M., Jiang, T., & Tsien, R. Y. (2009) · Integrative Biology · doi:10.1039/b904878b · Cited by 198
Self-citation
Cited by
Cell-Penetrating Peptides: Design, Synthesis, and Applications
Copolovici, D. M., Langel, K., Eriste, E., & Langel, Ü. (2014) · ACS Nano · doi:10.1021/nn4057269 · Cited by 963
Wang, Y., Zhou, K., Huang, G., Hensley, C., Huang, X., Ma, X., Zhao, T., Sumer, B. D., DeBerardinis, R. J., & Gao, J. (2014) · Nature Materials · doi:10.1038/nmat3819 · Cited by 773
Enzyme-responsive nanomaterials for controlled drug delivery
Hu, Q., Katti, P. S., & Gu, Z. (2014) · Nanoscale · doi:10.1039/c4nr04249b · Cited by 668
Aptamers from Cell-Based Selection for Bioanalytical Applications
Tan, W., Donovan, M. J., & Jiang, J. (2013) · Chemical Reviews · doi:10.1021/cr300468w · Cited by 649
Mechanisms of drug release in nanotherapeutic delivery systems
Wong, P. T., & Choi, S. K. (2015) · Chemical Reviews · doi:10.1021/cr5004634 · Cited by 507
Metadata
- Authors
- Olson, E. S., Jiang, T., Aguilera, T. A., Nguyen, Q. T., Ellies, L. G., Scadeng, M., & Tsien, R. Y.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-02-16
- Volume
- 107
- Issue
- 9
- Pages
- 4311-4316
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2046314109
- Cited by
- 567
- References
- 22
- FWCI
- 32.77
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases. https://app.cochat.ai/papers/10.1073/pnas.0910283107
- The paper
- Olson, E. S., Jiang, T., Aguilera, T. A., Nguyen, Q. T., Ellies, L. G., Scadeng, M., & Tsien, R. Y. (2010). Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0910283107
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
