The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Reengineering orthogonally selective riboswitches
Open accessAbstract
The ability to independently control the expression of multiple genes by addition of distinct small-molecule modulators has many applications from synthetic biology, functional genomics, pharmaceutical target validation, through to gene therapy. Riboswitches are relatively simple, small-molecule–dependent, protein-free, mRNA genetic switches that are attractive targets for reengineering in this context. Using a combination of chemical genetics and genetic selection, we have developed riboswitches that are selective for synthetic “nonnatural” small molecules and no longer respond to the natural intracellular ligands. The orthogonal selectivity of the riboswitches is also demonstrated in vitro using isothermal titration calorimetry and x-ray crystallography. The riboswitches allow highly responsive, dose-dependent, orthogonally selective, and dynamic control of gene expression in vivo. It is possible that this approach may be further developed to reengineer other natural riboswitches for application as small-molecule responsive genetic switches in both prokaryotes and eukaryotes.
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Cites
Mfold web server for nucleic acid folding and hybridization prediction
Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
Adenine riboswitches and gene activation by disruption of a transcription terminator
Mandal, M., & Breaker, R. R. (2004) · Nature Structural & Molecular Biology · doi:10.1038/nsmb710 · Cited by 514
Cited by
Serganov, A., & Nudler, E. (2013) · Cell · doi:10.1016/j.cell.2012.12.024 · Cited by 1,013
Currin, A., Swainston, N., Day, P. J., & Kell, D. B. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00351a · Cited by 388
Metabolite recognition principles and molecular mechanisms underlying riboswitch function
Serganov, A., & Patel, D. J. (2012) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-101211-113224 · Cited by 160
E. coli strain engineering for the production of advanced biopharmaceutical products
Selas Castiñeiras, T., Williams, S. G., Hitchcock, A. G., & Smith, D. C. (2018) · FEMS Microbiology Letters · doi:10.1093/femsle/fny162 · Cited by 92
Modular riboswitch toolsets for synthetic genetic control in diverse bacterial species
Robinson, C. J., Vincent, H. A., Wu, M.-C., Lowe, P. T., Dunstan, M. S., Leys, D., & Micklefield, J. (2014) · Journal of the American Chemical Society · doi:10.1021/ja502873j · Cited by 75
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Metadata
- Authors
- Dixon, N., Duncan, J. N., Geerlings, T., Dunstan, M. S., McCarthy, J. E. G., Leys, D., & Micklefield, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-01-26
- Volume
- 107
- Issue
- 7
- Pages
- 2830-2835
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2092435968
- Cited by
- 168
- References
- 2
- FWCI
- 5.91
- Type
- journal article
- Language
- en
- Open access
- green
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- Dixon, N., Duncan, J. N., Geerlings, T., Dunstan, M. S., McCarthy, J. E. G., Leys, D., & Micklefield, J. (2010). Reengineering orthogonally selective riboswitches. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0911209107
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