The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli
Open accessAbstract
Extraintestinal pathogenic Escherichia coli (ExPEC) are a common cause of disease in both mammals and birds. A vaccine to prevent such infections would be desirable given the increasing antibiotic resistance of these bacteria. We have determined the genome sequence of ExPEC IHE3034 (ST95) isolated from a case of neonatal meningitis and compared this to available genome sequences of other ExPEC strains and a few nonpathogenic E. coli . We found 19 genomic islands present in the genome of IHE3034, which are absent in the nonpathogenic E. coli isolates. By using subtractive reverse vaccinology we identified 230 antigens present in ExPEC but absent (or present with low similarity) in nonpathogenic strains. Nine antigens were protective in a mouse challenge model. Some of them were also present in other pathogenic non-ExPEC strains, suggesting that a broadly protective E. coli vaccine may be possible. The gene encoding the most protective antigen was detected in most of the E. coli isolates, highly conserved in sequence and found to be exported by a type II secretion system which seems to be nonfunctional in nonpathogenic strains.
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Cites
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Metadata
- Authors
- Moriel, D. G., Bertoldi, I., Spagnuolo, A., Marchi, S., Rosini, R., Nesta, B., Pastorello, I., Corea, V. A. M., Torricelli, G., Cartocci, E., Savino, S., Scarselli, M., Dobrindt, U., Hacker, J., Tettelin, H., Tallon, L. J., Sullivan, S., Wieler, L. H., Ewers, C., Pickard, D., Dougan, G., Fontana, M. R., Rappuoli, R., Pizza, M., & Serino, L.
- Affiliations
- Novartis (Italy) (IT)
- University of Würzburg (DE)
- University of Maryland, Baltimore (US)
- J. Craig Venter Institute (US)
- Freie Universität Berlin (DE)
- Wellcome Sanger Institute (GB)
- Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2010-05-03
- Volume
- 107
- Issue
- 20
- Pages
- 9072-9077
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2889118
- OpenAlex
- W1972223538
- Cited by
- 261
- References
- 54
- Citations by year
- 2026: 8 · 2025: 12 · 2024: 13 · 2023: 13 · 2022: 4 · 2021: 15 · 2020: 18 · 2019: 10 · 2018: 20 · 2017: 10 · 2016: 28 · 2015: 19 · 2014: 20 · 2013: 28 · 2012: 26
- FWCI
- 15.11
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Bundesministerium für Bildung und Forschung (01KI07120)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Keywords
- extraintestinal pathogenic Escherichia coli, vaccine antigens, genomic islands, neonatal meningitis, ST95, type II secretion system, conserved antigens, mouse challenge protection, genome sequence
- MeSH terms
- Animals, Antigens, Bacterial, Base Sequence, Escherichia coli, Escherichia coli Infections, Finland, Humans, Molecular Sequence Data, Genome, Bacterial, Sequence Analysis, DNA, Computational Biology, Meningitis, Escherichia coli, Escherichia coli Vaccines, Genomic Islands, Mice, Secretory Pathway
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