Cited by 261Escherichia coli research studiesvaccines and immunoinformatics approachesMicrobial infections and disease research

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Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli

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Moriel, Danilo Gomes, Bertoldi, Isabella, Spagnuolo, Angela, Marchi, Sara, Rosini, Roberto, Nesta, Barbara, Pastorello, Ilaria, Corea, Vanja A. Mariani, Torricelli, Giulia, Cartocci, Elena, Savino, Silvana, Scarselli, Maria, Dobrindt, Ulrich, Hacker, Jörg, Tettelin, Hervé, Tallon, Luke J., Sullivan, Steven, Wieler, Lothar H., Ewers, Christa, Pickard, Derek, Dougan, Gordon, Fontana, Maria Rita, Rappuoli, Rino, Pizza, Mariagrazia, Serino, Laura·2010·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.0915077107PMC PMC2889118

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Abstract

Extraintestinal pathogenic Escherichia coli (ExPEC) are a common cause of disease in both mammals and birds. A vaccine to prevent such infections would be desirable given the increasing antibiotic resistance of these bacteria. We have determined the genome sequence of ExPEC IHE3034 (ST95) isolated from a case of neonatal meningitis and compared this to available genome sequences of other ExPEC strains and a few nonpathogenic E. coli . We found 19 genomic islands present in the genome of IHE3034, which are absent in the nonpathogenic E. coli isolates. By using subtractive reverse vaccinology we identified 230 antigens present in ExPEC but absent (or present with low similarity) in nonpathogenic strains. Nine antigens were protective in a mouse challenge model. Some of them were also present in other pathogenic non-ExPEC strains, suggesting that a broadly protective E. coli vaccine may be possible. The gene encoding the most protective antigen was detected in most of the E. coli isolates, highly conserved in sequence and found to be exported by a type II secretion system which seems to be nonfunctional in nonpathogenic strains.

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Authors: Moriel, D. G., Bertoldi, I., Spagnuolo, A., Marchi, S., Rosini, R., Nesta, B., Pastorello, I., Corea, V. A. M., Torricelli, G., Cartocci, E., Savino, S., Scarselli, M., Dobrindt, U., Hacker, J., Tettelin, H., Tallon, L. J., Sullivan, S., Wieler, L. H., Ewers, C., Pickard, D., Dougan, G., Fontana, M. R., Rappuoli, R., Pizza, M., & Serino, L.
Affiliations: Novartis (Italy) (IT)
University of Würzburg (DE)
University of Maryland, Baltimore (US)
J. Craig Venter Institute (US)
Freie Universität Berlin (DE)
Wellcome Sanger Institute (GB)
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
ORCID: Moriel, Danilo Gomes
Spagnuolo, Angela
Torricelli, Giulia
Cartocci, Elena
Savino, Silvana
Scarselli, Maria
Dobrindt, Ulrich
Tettelin, Hervé
Tallon, Luke J.
Sullivan, Steven
Wieler, Lothar H.
Ewers, Christa
Rappuoli, Rino
Pizza, Mariagrazia
Serino, Laura
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2010-05-03
Volume: 107
Issue: 20
Pages: 9072-9077
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.0915077107
PMC: PMC2889118
OpenAlex: W1972223538
Cited by: 261
References: 54
Citations by year: 2026: 8 · 2025: 12 · 2024: 13 · 2023: 13 · 2022: 4 · 2021: 15 · 2020: 18 · 2019: 10 · 2018: 20 · 2017: 10 · 2016: 28 · 2015: 19 · 2014: 20 · 2013: 28 · 2012: 26
FWCI: 15.11
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Wellcome Trust
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Bundesministerium für Bildung und Forschung (01KI07120)
Topics: Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords: extraintestinal pathogenic Escherichia coli, vaccine antigens, genomic islands, neonatal meningitis, ST95, type II secretion system, conserved antigens, mouse challenge protection, genome sequence
MeSH terms: Animals, Antigens, Bacterial, Base Sequence, Escherichia coli, Escherichia coli Infections, Finland, Humans, Molecular Sequence Data, Genome, Bacterial, Sequence Analysis, DNA, Computational Biology, Meningitis, Escherichia coli, Escherichia coli Vaccines, Genomic Islands, Mice, Secretory Pathway

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Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli

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