The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors
Open accessAbstract
Human embryonic stem cells (hESCs) hold enormous promise for regenerative medicine. Typically, hESC-based applications would require their in vitro differentiation into a desirable homogenous cell population. A major challenge of the current hESC differentiation paradigm is the inability to effectively capture and, in the long-term, stably expand primitive lineage-specific stem/precursor cells that retain broad differentiation potential and, more importantly, developmental stage-specific differentiation propensity. Here, we report synergistic inhibition of glycogen synthase kinase 3 (GSK3), transforming growth factor β (TGF-β), and Notch signaling pathways by small molecules can efficiently convert monolayer cultured hESCs into homogenous primitive neuroepithelium within 1 wk under chemically defined condition. These primitive neuroepithelia can stably self-renew in the presence of leukemia inhibitory factor, GSK3 inhibitor (CHIR99021), and TGF-β receptor inhibitor (SB431542); retain high neurogenic potential and responsiveness to instructive neural patterning cues toward midbrain and hindbrain neuronal subtypes; and exhibit in vivo integration. Our work uniformly captures and maintains primitive neural stem cells from hESCs.
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Cites
Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
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GSK3 signalling in neural development
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Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy
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Direct Neural Fate Specification from Embryonic Stem Cells
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Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage
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Cited by
Sellgren, C. M., Gracias, J., Watmuff, B., Biag, J. D., Thanos, J. M., Whittredge, P. B., Fu, T., Worringer, K., Brown, H. E., Wang, J., et al. (2019) · Nature Neuroscience · doi:10.1038/s41593-018-0334-7 · Cited by 786
Distinct Lineage Specification Roles for NANOG, OCT4, and SOX2 in Human Embryonic Stem Cells
Wang, Z., Oron, E., Nelson, B., Razis, S., & Ivanova, N. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2012.02.016 · Cited by 554
Small molecules enhance CRISPR genome editing in pluripotent stem cells
Yu, C., Liu, Y., Ma, T., Liu, K., Xu, S., Zhang, Y., Liu, H., La Russa, M., Xie, M., Ding, S., et al. (2015) · Cell stem cell · doi:10.1016/j.stem.2015.01.003 · Cited by 455
Self-citationGSK-3 Inhibitors: Preclinical and Clinical Focus on CNS
Eldar-Finkelman, H., & Martinez, A. (2011) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2011.00032 · Cited by 354
Progressive degeneration of human neural stem cells caused by pathogenic LRRK2
Liu, G.-H., Qu, J., Suzuki, K., Nivet, E., Li, M., Montserrat, N., Yi, F., Xu, X., Ruiz, S., Zhang, W., et al. (2012) · Nature · doi:10.1038/nature11557 · Cited by 339
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Metadata
- Authors
- Li, W., Sun, W., Zhang, Y., Wei, W., Ambasudhan, R., Xia, P., Talantova, M., Lin, T., Kim, J., Wang, X., Kim, W. R., Lipton, S. A., Zhang, K., & Ding, S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-04-27
- Volume
- 108
- Issue
- 20
- Pages
- 8299-8304
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2168250872
- Cited by
- 375
- References
- 32
- FWCI
- 9.64
- Type
- journal article
- Language
- en
- Open access
- green
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- Li, W., Sun, W., Zhang, Y., Wei, W., Ambasudhan, R., Xia, P., Talantova, M., Lin, T., Kim, J., Wang, X., Kim, W. R., Lipton, S. A., Zhang, K., & Ding, S. (2011). Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1014041108
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