Cited by 322Cancer Cells and Metastasis3D Printing in Biomedical ResearchMathematical Biology Tumor Growth

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Retaining cell–cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer

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Kondo, Jumpei, Endo, Hiroko, Okuyama, Hiroaki, Ishikawa, Osamu, Iishi, Hiroyasu, Tsujii, Masahiko, Ohue, Masayuki, Inoue, Masahiro·2011·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1015938108PMC PMC3076886

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Abstract

Primary culture of the cancer cells from patients’ tumors can provide crucial information of individual tumors, yet the technology has not been optimized until now. We developed an innovative culture method for primary colorectal cancer cells, based on the principle that cell–cell contact of cancer cells was maintained throughout the process. When tumor tissue was dissociated into cell clusters, in which cell–cell contact was retained, they rapidly formed spheroids that we termed cancer tissue-originated spheroids (CTOSs). CTOSs of colorectal cancer consisted of highly purified and viable cancer cells, and they were prepared with high efficiency. In immunodeficient mice, CTOSs formed xenograft tumors that retained the features of the parental tumors. Moreover, CTOSs were able to be cultured and expanded in vitro using a 3D culture system and stem cell culture medium. This method allowed evaluation of chemosensitivity and signal pathway activation in cancer cells from individual patients. Easy preparation and culture of pure primary cancer cells provides an innovative platform for studying cancer biology and developing personalized medicine.

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Authors: Kondo, J., Endo, H., Okuyama, H., Ishikawa, O., Iishi, H., Tsujii, M., Ohue, M., & Inoue, M.
Affiliations: Osaka International Cancer Institute (JP)
University of California, San Francisco (US)
Surgery, and
The University of Osaka (JP)
ORCID: Kondo, Jumpei
Ishikawa, Osamu
Iishi, Hiroyasu
Ohue, Masayuki
Inoue, Masahiro
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2011-03-28
Volume: 108
Issue: 15
Pages: 6235-6240
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1015938108
PMC: PMC3076886
OpenAlex: W2043079400
Cited by: 322
References: 39
Citations by year: 2026: 12 · 2025: 16 · 2024: 24 · 2023: 29 · 2022: 29 · 2021: 33 · 2020: 28 · 2019: 25 · 2018: 29 · 2017: 23 · 2016: 25 · 2015: 21 · 2014: 15 · 2013: 10 · 2012: 2
FWCI: 4.40
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Ministry of Education, Culture, Sports, Science and Technology
Japan Society for the Promotion of Science (22791240)
Topics: Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Physical Sciences › Mathematics › Modeling and Simulation › Mathematical Biology Tumor Growth
Keywords: colorectal cancer, cell-cell contact, tumor cell dissociation, tumor xenografts, 3D cell culture, cancer stem cells, chemosensitivity, personalized medicine, tumor microenvironment
MeSH terms: Animals, Cell Communication, Cell Survival, Humans, Tumor Cells, Cultured, Colorectal Neoplasms, Cadherins, Spheroids, Cellular, Cell Culture Techniques, Xenograft Model Antitumor Assays, Mice

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Retaining cell–cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer

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