The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization
Open accessAbstract
To fuse with oocytes, spermatozoa of eutherian mammals must pass through extracellular coats, the cumulus cell layer, and the zona pellucida (ZP). It is generally believed that the acrosome reaction (AR) of spermatozoa, essential for zona penetration and fusion with oocytes, is triggered by sperm contact with the zona pellucida. Therefore, in most previous studies of sperm–oocyte interactions in the mouse, the cumulus has been removed before insemination to facilitate the examination of sperm–zona interactions. We used transgenic mouse spermatozoa, which enabled us to detect the onset of the acrosome reaction using fluorescence microscopy. We found that the spermatozoa that began the acrosome reaction before reaching the zona were able to penetrate the zona and fused with the oocyte's plasma membrane. In fact, most fertilizing spermatozoa underwent the acrosome reaction before reaching the zona pellucida of cumulus-enclosed oocytes, at least under the experimental conditions we used. The incidence of in vitro fertilization of cumulus-free oocytes was increased by coincubating oocytes with cumulus cells, suggesting an important role for cumulus cells and their matrix in natural fertilization.
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Cited by
Extracellular vesicles: roles in gamete maturation, fertilization and embryo implantation
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Endoplasmic Reticulum Stress and Homeostasis in Reproductive Physiology and Pathology
Guzel, E., Arlier, S., Guzeloglu-Kayisli, O., Tabak, M., Ekiz, T., Semerci, N., Larsen, K., Schatz, F., Lockwood, C., & Kayisli, U. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18040792 · Cited by 257
The functional anatomy of the human spermatozoon: relating ultrastructure and function
Mortimer, D. (2018) · MHR: Basic science of reproductive medicine · doi:10.1093/molehr/gay040 · Cited by 63
EDC IMPACT: Chemical UV filters can affect human sperm function in a progesterone-like manner
Rehfeld, A., Egeberg, D. L., Almstrup, K., Petersen, J. H., Dissing, S., & Skakkebæk, N. E. (2018) · Endocrine Connections · doi:10.1530/ec-17-0156 · Cited by 58
Metadata
- Authors
- Jin, M., Fujiwara, E., Kakiuchi, Y., Okabe, M., Satouh, Y., Baba, S. A., Chiba, K., & Hirohashi, N.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-03-07
- Volume
- 108
- Issue
- 12
- Pages
- 4892-4896
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2127489545
- Cited by
- 422
- References
- 19
- FWCI
- 42.71
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization. https://app.cochat.ai/papers/10.1073/pnas.1018202108
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- Jin, M., Fujiwara, E., Kakiuchi, Y., Okabe, M., Satouh, Y., Baba, S. A., Chiba, K., & Hirohashi, N. (2011). Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1018202108
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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