The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
The bacterial actin MreB rotates, and rotation depends on cell-wall assembly
Open accessAbstract
Bacterial cells possess multiple cytoskeletal proteins involved in a wide range of cellular processes. These cytoskeletal proteins are dynamic, but the driving forces and cellular functions of these dynamics remain poorly understood. Eukaryotic cytoskeletal dynamics are often driven by motor proteins, but in bacteria no motors that drive cytoskeletal motion have been identified to date. Here, we quantitatively study the dynamics of the Escherichia coli actin homolog MreB, which is essential for the maintenance of rod-like cell shape in bacteria. We find that MreB rotates around the long axis of the cell in a persistent manner. Whereas previous studies have suggested that MreB dynamics are driven by its own polymerization, we show that MreB rotation does not depend on its own polymerization but rather requires the assembly of the peptidoglycan cell wall. The cell-wall synthesis machinery thus either constitutes a novel type of extracellular motor that exerts force on cytoplasmic MreB, or is indirectly required for an as-yet-unidentified motor. Biophysical simulations suggest that one function of MreB rotation is to ensure a uniform distribution of new peptidoglycan insertion sites, a necessary condition to maintain rod shape during growth. These findings both broaden the view of cytoskeletal motors and deepen our understanding of the physical basis of bacterial morphogenesis.
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Regulation of peptidoglycan synthesis and remodelling
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Nonthermal ATP-dependent fluctuations contribute to the in vivo motion of chromosomal loci
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Metadata
- Authors
- van Teeffelen, S., Wang, S., Furchtgott, L., Huang, K. C., Wingreen, N. S., Shaevitz, J. W., & Gitai, Z.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-09-08
- Volume
- 108
- Issue
- 38
- Pages
- 15822-15827
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2095658523
- Cited by
- 417
- References
- 27
- FWCI
- 20.10
- Type
- journal article
- Language
- en
- Open access
- bronze
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- van Teeffelen, S., Wang, S., Furchtgott, L., Huang, K. C., Wingreen, N. S., Shaevitz, J. W., & Gitai, Z. (2011). The bacterial actin MreB rotates, and rotation depends on cell-wall assembly. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1108999108
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