The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Nonthermal ATP-dependent fluctuations contribute to the in vivo motion of chromosomal loci
Open accessAbstract
Chromosomal loci jiggle in place between segregation events in prokaryotic cells and during interphase in eukaryotic nuclei. This motion seems random and is often attributed to Brownian motion. However, we show here that locus dynamics in live bacteria and yeast are sensitive to metabolic activity. When ATP synthesis is inhibited, the apparent diffusion coefficient decreases, whereas the subdiffusive scaling exponent remains constant. Furthermore, the magnitude of locus motion increases more steeply with temperature in untreated cells than in ATP-depleted cells. This “superthermal” response suggests that untreated cells have an additional source of molecular agitation, beyond thermal motion, that increases sharply with temperature. Such ATP-dependent fluctuations are likely mechanical, because the heat dissipated from metabolic processes is insufficient to account for the difference in locus motion between untreated and ATP-depleted cells. Our data indicate that ATP-dependent enzymatic activity, in addition to thermal fluctuations, contributes to the molecular agitation driving random (sub)diffusive motion in the living cell.
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Cited by
The Bacterial Cytoplasm Has Glass-like Properties and Is Fluidized by Metabolic Activity
Parry, B. R., Surovtsev, I. V., Cabeen, M. T., O’Hern, C. S., Dufresne, E. R., & Jacobs-Wagner, C. (2014) · Cell · doi:10.1016/j.cell.2013.11.028 · Cited by 822
Transcription-coupled changes in nuclear mobility of mammalian cis-regulatory elements
Gu, B., Swigut, T., Spencley, A., Bauer, M. R., Chung, M., Meyer, T., & Wysocka, J. (2018) · Science · doi:10.1126/science.aao3136 · Cited by 363
Cytoplasmic organization promotes protein diffusion in Xenopus extracts
Huang, W. Y. C., Cheng, X., & Ferrell, J. E. (2022) · Nature Communications · doi:10.1038/s41467-022-33339-0 · Cited by 35
Loop-extruders alter bacterial chromosome topology to direct entropic forces for segregation
Harju, J., van Teeseling, M. C. F., & Broedersz, C. P. (2024) · Nature Communications · doi:10.1038/s41467-024-49039-w · Cited by 27
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Dynamics of chromosome organization in a minimal bacterial cell
Gilbert, B. R., Thornburg, Z. R., Brier, T. A., Stevens, J. A., Grünewald, F., Stone, J. E., Marrink, S. J., & Luthey-Schulten, Z. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2023.1214962 · Cited by 19
Metadata
- Authors
- Weber, S. C., Spakowitz, A. J., & Theriot, J. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2012-04-19
- Volume
- 109
- Issue
- 19
- Pages
- 7338-7343
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2053101927
- Cited by
- 344
- References
- 43
- FWCI
- 28.77
- Type
- journal article
- Language
- en
- Open access
- bronze
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