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Cited by 440Estrogen and related hormone effectsProstate Cancer Treatment and ResearchUrinary Bladder and Prostate Research

A role for estrogen receptor β in the regulation of growth of the ventral prostate

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Weihua, Zhang, Mäkelä, Sari, Andersson, Leif C., Salmi, Saija, Saji, Shigehira, Webster, Jeanette I., Jensen, Elwood V., Nilsson, Stefan +2 more·2001·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.111150898PMC PMC33468
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Abstract

In normal rats and mice, immunostaining with specific antibodies revealed that nuclei of most prostatic epithelial cells harbor estrogen receptor β (ERβ). In rat ventral prostate, 530- and 549-aa isoforms of the receptor were identified. These sediment in the 4S region of low-salt sucrose gradients, indicating that prostatic ERβ does not contain the same protein chaperones that are associated with ERα. Estradiol (E 2 ) binding and ERβ immunoreactivity coincide on the gradient, with no indication of ERα. In prostates from mice in which the ERβ gene has been inactivated (BERKO), androgen receptor (AR) levels are elevated, and the tissue contains multiple hyperplastic foci. Most epithelial cells express the proliferation antigen Ki-67 . In contrast, prostatic epithelium from wild-type littermates is single layered with no hyperplasia, and very few cells express Ki-67 . Rat ventral prostate contains an estrogenic component, which comigrates on HPLC with the testosterone metabolite 5α-androstane-3β,17β-diol (3βAdiol). This compound, which competes with E 2 for binding to ERβ and elicits an estrogenic response in the aorta but not in the pituitary, decreases the AR content in prostates of wild-type mice but does not affect the elevated levels seen in ERβ knockout (BERKO) mice. Thus ERβ, probably as a complex with 3βAdiol, is involved in regulating the AR content of the rodent prostate and in restraining epithelial growth. These findings suggest that ligands specific for ERβ may be useful in the prevention and/or clinical management of prostatic hyperplasia and neoplasia.

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  1. [1]Kozak I, Bartsch W, Krieg M, Voigt K D. Prostate. 1982;3:433–438. doi: 10.1002/pros.2990030503. [DOI] [PubMed] [Google Scholar] · DOI
  2. [2]Shao T C, Tindall D J, Cunningham G R. Prostate. 1986;8:349–362. doi: 10.1002/pros.2990080407. [DOI] [PubMed] [Google Scholar] · DOI
  3. [3]Martikainen P M, Mäkelä S I, Santti R S, Harkonen P L, Suominen J J. Prostate. 1987;11:291–303. doi: 10.1002/pros.2990110402. [DOI] [PubMed] [Google Scholar] · DOI
  4. [4]Kawamura H, Kimura M, Ichihara I. Anat Anz. 1993;175:569–575. doi: 10.1016/s0940-9602(11)80251-7. [DOI] [PubMed] [Google Scholar] · DOI
  5. [5]Krieg M, Weisser H, Tunn S. Verh Dtsch Ges Pathol. 1993;77:19–24. [PubMed] [Google Scholar]
  6. [6]Pylkkanen L, Mäkelä S, Santti R. Eur J Urol. 1996;30:243–248. doi: 10.1159/000474175. [DOI] [PubMed] [Google Scholar] · DOI
  7. [7]Olbina G, Miljkovic D, Hoffman R M, Geller J. Prostate. 1998;37:126–129. doi: 10.1002/(sici)1097-0045(19981001)37:2<126::aid-pros9>3.0.co;2-e. [DOI] [PubMed] [Google Scholar] · DOI
  8. [8]Messina M J, Persky V, Setchell K D, Barnes S. Nutr Cancer. 1994;21:113–131. doi: 10.1080/01635589409514310. [DOI] [PubMed] [Google Scholar] · DOI
  9. [9]Bosland M C, Ford H, Horton L. Carcinogenesis. 1995;16:1311–1317. doi: 10.1093/carcin/16.6.1311. [DOI] [PubMed] [Google Scholar] · DOI
  10. [10]Barnes S, Peterson T G, Coward L. J Cell Biochem. 1995;22:181–177. doi: 10.1002/jcb.240590823. [DOI] [PubMed] [Google Scholar] · DOI
  11. [11]Goodman M T, Wilkens L R, Hankin J H, Lyu L C, Wu A H, Kolonel L N. Am J Epidemiol. 1997;146:294–306. doi: 10.1093/oxfordjournals.aje.a009270. [DOI] [PubMed] [Google Scholar] · DOI
  12. [12]Landstrom M, Zhang J X, Hallmans G, Aman P, Bergh A, Damber J E, Mazur W, Wahala K, Adlercreutz H. Prostate. 1998;36:151–161. doi: 10.1002/(sici)1097-0045(19980801)36:3<151::aid-pros2>3.0.co;2-k. [DOI] [PubMed] [Google Scholar] · DOI
  13. [13]Hempstock J, Kavanagh J P, George N J. Br J Urol. 1998;82:560–563. doi: 10.1046/j.1464-410x.1998.00769.x. [DOI] [PubMed] [Google Scholar] · DOI
  14. [14]Paech K, Webb P, Kuiper G G, Nilsson S, Gustafsson J-Å, Kushner P J, Scanlan T S. Science. 1997;277:1508–1510. doi: 10.1126/science.277.5331.1508. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  15. [15]Weihua Z, Saji S, Mäkinen S, Cheng G, Jensen E V, Warner M, Gustafsson J-Å. Proc Natl Acad Sci USA. 2000;97:5936–5941. doi: 10.1073/pnas.97.11.5936. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI · Paper page
  16. [16]Ekman P, Barrack E R, Greene G L, Jensen E V, Walsh P C. J Clin Endocrinol Metab. 1983;57:166–176. doi: 10.1210/jcem-57-1-166. [DOI] [PubMed] [Google Scholar] · DOI
  17. [17]Bodker A, Andersson K E, Batra S, Juhl B R, Meyhoff H H. Scand J Urol Nephrol. 1993;27:169–174. doi: 10.3109/00365599309181244. [DOI] [PubMed] [Google Scholar] · DOI
  18. [18]Cooke P S, Buchanan D L, Young P, Setiawan T, Brody J, Korach K S, Taylor J, Lubahn D B, Cunha G R. Proc Natl Acad Sci USA. 1997;94:6535–6540. doi: 10.1073/pnas.94.12.6535. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  19. [19]Kuiper G G, Enmark E, Pelto-Huikko M, Nilsson S, Gustafsson J-Å. Proc Natl Acad Sci USA. 1996;93:5925–5930. doi: 10.1073/pnas.93.12.5925. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  20. [20]Prins G S, Marmer M, Woodham C, Chang W, Kuiper G G, Gustafsson J-Å, Birch L. Endocrinology. 1998;139:874–883. doi: 10.1210/endo.139.3.5827. [DOI] [PubMed] [Google Scholar] · DOI
  21. [21]Krege J H, Hodgin J B, Couse J F, Warner M, Mahler J F, Sar M, Korach K S, Gustafsson J-Å, Smithies O. Proc Natl Acad Sci USA. 1998;95:15677–15682. doi: 10.1073/pnas.95.26.15677. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  22. [22]Schneider M R, Hartmann R W, Sinowatz F, Amselgruber W. J Cancer Res Clin Oncol. 1986;112:258–265. doi: 10.1007/BF00395920. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  23. [23]Lamartiniere C A, Zhang J X, Cotroneo M S. Am J Clin Nutr. 1998;68:1400.S–1405S. doi: 10.1093/ajcn/68.6.1400S. [DOI] [PubMed] [Google Scholar] · DOI
  24. [24]Voigt K-D, Bartsch W. J Steroid Biochem. 1986;25:749–757. doi: 10.1016/0022-4731(86)90304-3. [DOI] [PubMed] [Google Scholar] · DOI
  25. [25]Kuiper G G, Carlsson B, Grandien K, Enmark E, Haggblad J, Nilsson S, Gustafsson J-Å. Endocrinology. 1998;138:863–870. doi: 10.1210/endo.138.3.4979. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  26. [26]Turcotte G, Chapdelaine A, Roberts K D, Chevalier S. J Steroid Biochem. 1988;29:69–76. doi: 10.1016/0022-4731(88)90378-0. [DOI] [PubMed] [Google Scholar] · DOI
  27. [27]Saji S, Jensen E V, Nilsson S, Rylander T, Warner M, Gustafsson J-Å. Proc Natl Acad Sci USA. 2000;97:337–342. doi: 10.1073/pnas.97.1.337. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  28. [28]Jensen E V, Suzuki T, Kawashima T, Stumpf W E, Jungblut P W, DeSombre E R. Proc Natl Acad Sci USA. 1968;59:632–638. doi: 10.1073/pnas.59.2.632. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  29. [29]Barkhem T, Carlsson B, Nilsson Y, Enmark E, Gustafsson J-Å, Nilsson S. Mol Pharmacol. 1998;54:105–112. doi: 10.1124/mol.54.1.105. [DOI] [PubMed] [Google Scholar] · DOI
  30. [30]Gerdes J, Lemke H, Baisch H, Wacker H H, Schwab U, Stein H. J Immunol. 1984;133:1710–1715. [PubMed] [Google Scholar]
  31. [31]Pratt W B, Toft D O. Endocr Rev. 1997;18:306–360. doi: 10.1210/edrv.18.3.0303. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  32. [32]Sultan C, Terouanne B, Tahiri B, Lumbroso S, Avances C, Orio F., Jr Bull Cancer. 1999;86:618–621. [PubMed] [Google Scholar]
  33. [33]Gann P H, Hennekens C H, Ma J, Longcope C, Stampfer M J. J Natl Cancer Inst. 1996;88:1118–1126. doi: 10.1093/jnci/88.16.1118. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  34. [34]Gustafsson O, Norming U, Gustafsson S, Eneroth P, Astrom G, Nyman C R. Br J Urol. 1996;77:433–440. doi: 10.1046/j.1464-410x.1996.89120.x. [DOI] [PubMed] [Google Scholar] · DOI
  35. [35]Vatten L J, Ursin G, Ross R K, Stanczyk F Z, Lobo R A, Harvei S, Jellum E. Cancer Epidemiol Biomarkers Prevention. 1997;6:967–969. [PubMed] [Google Scholar]

Cited by

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Authors
Weihua, Z., Mäkelä, S., Andersson, L. C., Salmi, S., Saji, S., Webster, J. I., Jensen, E. V., Nilsson, S., Warner, M., & Gustafsson, J.-Å.
Affiliations
  • University of Helsinki (FI)
  • Houston Methodist (US)
  • Departments of Medical Nutrition and Biosciences, Karolinska Institute, Novum, Huddinge, S-141 86 Sweden; Institute of Biomedicine, Department of Anatomy, University of Turku, Turku FIN-20520, Finland; Department of Pathology, Haartman Institute, University of Helsinki, Helsinki FIN-00014, Finland; Department of Oncology and Pathology, Karolinska Institute, Huddinge, S-171 76 Sweden; and KaroBio AB, Novum, Huddinge S-141 86, Sweden
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2001-05-22
Volume
98
Issue
11
Pages
6330-6335
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.111150898
PMC
PMC33468
OpenAlex
W2058177423
Cited by
440
References
1
Citations by year
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FWCI
19.53
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Johns Hopkins University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Prostate Cancer Treatment and Research
  • Health Sciences › Medicine › Urology › Urinary Bladder and Prostate Research
Keywords
estrogen receptor beta, ventral prostate, prostatic hyperplasia, androgen receptor, estrogenic activity, Ki-67, ERβ knockout mice, prostatic neoplasia, ERβ isoforms, molecular chaperones
MeSH terms
Animals, Centrifugation, Density Gradient, Estrogens, Male, Prostate, Receptors, Estrogen, Sucrose, Androstane-3,17-diol, Rats, Sprague-Dawley, Mice, Knockout, Ki-67 Antigen, Protein Isoforms, Estrogen Receptor beta, Mice, Rats

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