Energetics of stalk intermediates in membrane fusion are controlled by lipid composition
Open accessAbstract
We have used X-ray diffraction on the rhombohedral phospholipid phase to reconstruct stalk structures in different pure lipids and lipid mixtures with unprecedented resolution, enabling a quantitative analysis of geometry, as well as curvature and hydration energies. Electron density isosurfaces are used to study shape and curvature properties of the bent lipid monolayers. We observe that the stalk structure is highly universal in different lipid systems. The associated curvatures change in a subtle, but systematic fashion upon changes in lipid composition. In addition, we have studied the hydration interaction prior to the transition from the lamellar to the stalk phase. The results indicate that facilitating dehydration is the key to promote stalk formation, which becomes favorable at an approximately constant interbilayer separation of 9.0 ± 0.5 Å for the investigated lipid compositions.
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This morphological change of the plasma membrane juxtaposes the membranes closer than the critical distance (0.9 nm) to promote stalk formation, and subsequently leads to fusion pore opening.
“This morphological change of the plasma membrane juxtaposes the membranes closer than the critical distance (0.9 nm) to promote stalk formation48 (Fig. 5d), and subsequently leads to fusion pore opening (Fig. 5e).”
From Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis (2015) · cites this paper as [48]
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“, HIV) membrane fusion involve the formation of helix bundles (68, 69).”
BackgroundMolecular anatomy of a trafficking organelle
Takamori, S., Holt, M., Stenius, K., Lemke, E. A., Grønborg, M., Riedel, D., Urlaub, H., Schenck, S., Brügger, B., Ringler, P., et al. (2006) · Cell · doi:10.1016/j.cell.2006.10.030 · Cited by 2,373 · see citing works
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The role of cholesterol in membrane fusion
Yang, S.-T., Kreutzberger, A. J. B., Lee, J., Kiessling, V., & Tamm, L. K. (2016) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2016.05.003 · Cited by 421 · see citing works
Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis
Zhou, Q., Lai, Y., Bacaj, T., Zhao, M., Lyubimov, A. Y., Uervirojnangkoorn, M., Zeldin, O. B., Brewster, A. S., Sauter, N. K., Cohen, A. E., et al. (2015) · Nature · doi:10.1038/nature14975 · Cited by 348 · see citing works
- Authors
- Aeffner, S., Reusch, T., Weinhausen, B., & Salditt, T.
- Affiliations
- University of Göttingen (DE)
- ORCID
- Salditt, Tim
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2012-05-15
- Volume
- 109
- Issue
- 25
- Pages
- E1609-18
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3382523
- OpenAlex
- W2014908030
- Cited by
- 185
- References
- 3
- Citations by year
- 2026: 13 · 2025: 9 · 2024: 13 · 2023: 16 · 2022: 15 · 2021: 19 · 2020: 14 · 2019: 6 · 2018: 17 · 2017: 16 · 2016: 11 · 2015: 12 · 2014: 13 · 2013: 9 · 2012: 2
- FWCI
- 4.60
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DORA PSI (Paul Scherrer Institute) (submittedVersion)
- GRO (submittedVersion, cc-by)
- Funders
- Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und Molekulare Biowissenschaften
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- membrane fusion, rhombohedral phase, lipid composition, curvature energy, hydration energy, dehydration, lipid mixtures
- MeSH terms
- Energy Metabolism, Lipids, Membrane Fusion, Water, X-Ray Diffraction
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