The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Krüppel-like Factor 7 engineered for transcriptional activation promotes axon regeneration in the adult corticospinal tract
Open accessAbstract
Axon regeneration in the central nervous system normally fails, in part because of a developmental decline in the intrinsic ability of CNS projection neurons to extend axons. Members of the KLF family of transcription factors regulate regenerative potential in developing CNS neurons. Expression of one family member, KLF7, is down-regulated developmentally, and overexpression of KLF7 in cortical neurons in vitro promotes axonal growth. To circumvent difficulties in achieving high neuronal expression of exogenous KLF7, we created a chimera with the VP16 transactivation domain, which displayed enhanced neuronal expression compared with the native protein while maintaining transcriptional activation and growth promotion in vitro. Overexpression of VP16-KLF7 overcame the developmental loss of regenerative ability in cortical slice cultures. Adult corticospinal tract (CST) neurons failed to up-regulate KLF7 in response to axon injury, and overexpression of VP16-KLF7 in vivo promoted both sprouting and regenerative axon growth in the CST of adult mice. These findings identify a unique means of promoting CST axon regeneration in vivo by reengineering a developmentally down-regulated, growth-promoting transcription factor.
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Cites
A new mathematical model for relative quantification in real-time RT-PCR
Pfaffl, M. W. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.9.e45 · Cited by 34,610
Chondroitinase ABC promotes functional recovery after spinal cord injury
Bradbury, E. J., Moon, L. D. F., Popat, R. J., King, V. R., Bennett, G. S., Patel, P. N., Fawcett, J. W., & McMahon, S. B. (2002) · Nature · doi:10.1038/416636a · Cited by 2,357
Promoting Axon Regeneration in the Adult CNS by Modulation of the PTEN/mTOR Pathway
Park, K. K., Liu, K., Hu, Y., Smith, P. D., Wang, C., Cai, B., Xu, B., Connolly, L., Kramvis, I., Sahin, M., et al. (2008) · Science · doi:10.1126/science.1161566 · Cited by 1,686
GAL4-VP16 is an unusually potent transcriptional activator
Sadowski, I., Ma, J., Triezenberg, S., & Ptashne, M. (1988) · Nature · doi:10.1038/335563a0 · Cited by 1,333
Neuronal Subtype-Specific Genes that Control Corticospinal Motor Neuron Development In Vivo
Arlotta, P., Molyneaux, B. J., Chen, J., Inoue, J., Kominami, R., & Macklis, J. D. (2005) · Neuron · doi:10.1016/j.neuron.2004.12.036 · Cited by 1,154
PTEN deletion enhances the regenerative ability of adult corticospinal neurons
Liu, K., Lu, Y., Lee, J. K., Samara, R., Willenberg, R., Sears-Kraxberger, I., Tedeschi, A., Park, K. K., Jin, D., Cai, B., et al. (2010) · Nature Neuroscience · doi:10.1038/nn.2603 · Cited by 985
Self-citationSustained axon regeneration induced by co-deletion of PTEN and SOCS3
Sun, F., Park, K. K., Belin, S., Wang, D., Lu, T., Chen, G., Zhang, K., Yeung, C., Feng, G., Yankner, B. A., et al. (2011) · Nature · doi:10.1038/nature10594 · Cited by 754
cAMP and Schwann cells promote axonal growth and functional recovery after spinal cord injury
Pearse, D. D., Pereira, F. C., Marcillo, A. E., Bates, M. L., Berrocal, Y. A., Filbin, M. T., & Bunge, M. B. (2004) · Nature Medicine · doi:10.1038/nm1056 · Cited by 727
KLF Family Members Regulate Intrinsic Axon Regeneration Ability
Moore, D. L., Blackmore, M. G., Hu, Y., Kaestner, K. H., Bixby, J. L., Lemmon, V. P., & Goldberg, J. L. (2009) · Science · doi:10.1126/science.1175737 · Cited by 714
Self-citationGrill, R., Murai, K., Blesch, A., Gage, F. H., & Tuszynski, M. H. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-14-05560.1997 · Cited by 618
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Regulation of axonal regeneration after mammalian spinal cord injury
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Restoring function after spinal cord injury: towards clinical translation of experimental strategies
Ramer, L. M., Ramer, M. S., & Bradbury, E. J. (2014) · The Lancet Neurology · doi:10.1016/s1474-4422(14)70144-9 · Cited by 324
An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury
Hilton, B. J., Husch, A., Schaffran, B., Lin, T.-C., Burnside, E. R., Dupraz, S., Schelski, M., Kim, J., Müller, J. A., Schoch, S., et al. (2022) · Neuron · doi:10.1016/j.neuron.2021.10.007 · Cited by 116
Wong, K. A., & Benowitz, L. I. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231710179 · Cited by 66
Ajoy, R., Lo, Y.-C., Ho, M.-H., Chen, Y.-Y., Wang, Y., Chen, Y.-H., Jing-Yuan, C., Changou, C. A., Hsiung, Y.-C., Chen, H.-M., et al. (2021) · Molecular Psychiatry · doi:10.1038/s41380-021-01103-3 · Cited by 57
Metadata
- Authors
- Blackmore, M. G., Wang, Z., Lerch, J. K., Motti, D., Zhang, Y. P., Shields, C. B., Lee, J. K., Goldberg, J. L., Lemmon, V. P., & Bixby, J. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2012-04-23
- Volume
- 109
- Issue
- 19
- Pages
- 7517-7522
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2002314797
- Cited by
- 272
- References
- 52
- FWCI
- 6.25
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Krüppel-like Factor 7 engineered for transcriptional activation promotes axon regeneration in the adult corticospinal tract. https://app.cochat.ai/papers/10.1073/pnas.1120684109
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- Blackmore, M. G., Wang, Z., Lerch, J. K., Motti, D., Zhang, Y. P., Shields, C. B., Lee, J. K., Goldberg, J. L., Lemmon, V. P., & Bixby, J. L. (2012). Krüppel-like Factor 7 engineered for transcriptional activation promotes axon regeneration in the adult corticospinal tract. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1120684109
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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