The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Cyclooxygenase-2 expression is induced during human megakaryopoiesis and characterizes newly formed platelets
Open accessAbstract
Cyclooxygenase (COX)-1 or -2 and prostaglandin (PG) synthases catalyze the formation of various PGs and thromboxane (TX) A 2 . We have investigated the expression and activity of COX-1 and -2 during human megakaryocytopoiesis. We analyzed megakaryocytes from bone marrow biopsies and derived from thrombopoietin-treated CD34 + hemopoietic progenitor cells in culture. Platelets were obtained from healthy donors and patients with high platelet regeneration because of immune thrombocytopenia or peripheral blood stem cell transplantation. By immunocytochemistry, COX-1 was observed in CD34 + cells and in megakaryocytes at each stage of maturation, whereas COX-2 was induced after 6 days of culture, and remained detectable in mature megakaryocytes. CD34 + cells synthesized more PGE 2 than TXB 2 (214 ± 50 vs. 30 ± 10 pg/10 6 cells), whereas the reverse was true in mature megakaryocytes (TXB 2 8,440 ± 2,500 vs. PGE 2 906 ± 161 pg/10 6 cells). By immunostaining, COX-2 was observed in <10% of circulating platelets from healthy controls, whereas up to 60% of COX-2-positive platelets were found in patients. A selective COX-2 inhibitor reduced platelet production of both PGE 2 and TXB 2 to a significantly greater extent in patients than in healthy subjects. Finally, we found that COX-2 and the inducible PGE-synthase were coexpressed in mature megakaryocytes and in platelets. We conclude that both COX-isoforms contribute to prostanoid formation during human megakaryocytopoiesis and that COX-2-derived PGE 2 and TXA 2 may play an unrecognized role in inflammatory and hemostatic responses in clinical syndromes associated with high platelet turnover.
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Cites
The Coxibs, Selective Inhibitors of Cyclooxygenase-2
FitzGerald, G. A., & Patrono, C. (2001) · New England Journal of Medicine · doi:10.1056/nejm200108093450607 · Cited by 1,565
Self-citationEikelboom, J. W., Hirsh, J., Weitz, J. I., Johnston, M., Yi, Q., & Yusuf, S. (2002) · Circulation · doi:10.1161/01.cir.0000013777.21160.07 · Cited by 1,053
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Self-citationAspirin as an Antiplatelet Drug
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Self-citationPatrignani, P., Filabozzi, P., & Patrono, C. (1982) · Journal of Clinical Investigation · doi:10.1172/jci110576 · Cited by 937
Self-citationActivated platelets mediate inflammatory signaling by regulated interleukin 1β synthesis
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Low dose aspirin and inhibition of thromboxane B2 production in healthy subjects
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Self-citationWhy there are two cyclooxygenase isozymes
Smith, W. L., & Langenbach, R. (2001) · Journal of Clinical Investigation · doi:10.1172/jci13271 · Cited by 611
Characterization of the human gene (PTGS2) encoding prostaglandin‐endoperoxide synthase 2
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Cited by
Platelet Activation and Atherothrombosis
Davì, G., & Patrono, C. (2007) · New England Journal of Medicine · doi:10.1056/nejmra071014 · Cited by 2,135
Self-citationProstaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis
North, T. E., Goessling, W., Walkley, C. R., Lengerke, C., Kopani, K. R., Lord, A. M., Weber, G. J., Bowman, T. V., Jang, I.-H., Grosser, T., et al. (2007) · Nature · doi:10.1038/nature05883 · Cited by 1,029
Patrono, C., Coller, B., Dalen, J. E., Fitz Gerald, G. A., Fuster, V., Gent, M., Hirsh, J., & Roth, G. (2001) · Chest · doi:10.1378/chest.119.1_suppl.39s · Cited by 963
Self-citationWeitz, J. I., Eikelboom, J. W., & Samama, M. M. (2012) · Chest · doi:10.1378/chest.11-2294 · Cited by 527
Guthikonda, S., Alviar, C. L., Vaduganathan, M., Arikan, M., Tellez, A., DeLao, T., Granada, J. F., Dong, J.-F., Kleiman, N. S., & Lev, E. I. (2008) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2008.05.031 · Cited by 337
Metadata
- Authors
- Rocca, B., Secchiero, P., Ciabattoni, G., Ranelletti, F. O., Catani, L., Guidotti, L., Melloni, E., Maggiano, N., Zauli, G., & Patrono, C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-05-21
- Volume
- 99
- Issue
- 11
- Pages
- 7634-7639
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2020789281
- Cited by
- 317
- References
- 29
- FWCI
- 9.10
- Type
- journal article
- Language
- en
- Open access
- green
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- Rocca, B., Secchiero, P., Ciabattoni, G., Ranelletti, F. O., Catani, L., Guidotti, L., Melloni, E., Maggiano, N., Zauli, G., & Patrono, C. (2002). Cyclooxygenase-2 expression is induced during human megakaryopoiesis and characterizes newly formed platelets. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.112202999
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