The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Using translational enhancers to increase transgene expression in Drosophila
Open accessAbstract
The ability to specify the expression levels of exogenous genes inserted in the genomes of transgenic animals is critical for the success of a wide variety of experimental manipulations. Protein production can be regulated at the level of transcription, mRNA transport, mRNA half-life, or translation efficiency. In this report, we show that several well-characterized sequence elements derived from plant and insect viruses are able to function in Drosophila to increase the apparent translational efficiency of mRNAs by as much as 20-fold. These increases render expression levels sufficient for genetic constructs previously requiring multiple copies to be effective in single copy, including constructs expressing the temperature-sensitive inactivator of neuronal function Shibire ts1 , and for the use of cytoplasmic GFP to image the fine processes of neurons.
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Cites
A dimorphic pheromone circuit in Drosophila from sensory input to descending output
Ruta, V., Datta, S. R., Vasconcelos, M. L., Freeland, J., Looger, L. L., & Axel, R. (2010) · Nature · doi:10.1038/nature09554 · Cited by 344
Waddell, S., Armstrong, J. D., Kitamoto, T., Kaiser, K., & Quinn, W. G. (2000) · Cell · doi:10.1016/s0092-8674(00)00183-5 · Cited by 332
Cited by
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Port, F., Chen, H.-M., Lee, T., & Bullock, S. L. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1405500111 · Cited by 1,046
Sun, F., Zeng, J., Jing, M., Zhou, J., Feng, J., Owen, S. F., Luo, Y., Li, F., Wang, H., Yamaguchi, T., et al. (2018) · Cell · doi:10.1016/j.cell.2018.06.042 · Cited by 837
Nern, A., Pfeiffer, B. D., & Rubin, G. M. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1506763112 · Cited by 584
Self-citationA multilevel multimodal circuit enhances action selection in drosophila
Ohyama, T., Schneider-Mizell, C. M., Fetter, R. D., Aleman, J. V., Franconville, R., Rivera-Alba, M., Mensh, B. D., Branson, K. M., Simpson, J. H., Truman, J. W., et al. (2015) · Nature · doi:10.1038/nature14297 · Cited by 502
Self-citationDrosophila neuroblasts: a model for stem cell biology
Homem, C. C. F., & Knoblich, J. A. (2012) · Development · doi:10.1242/dev.080515 · Cited by 476
Metadata
- Authors
- Pfeiffer, B. D., Truman, J. W., & Rubin, G. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2012-04-09
- Volume
- 109
- Issue
- 17
- Pages
- 6626-6631
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2025654941
- Cited by
- 395
- References
- 2
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- green
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