The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Conjugation to gold nanoparticles enhances polyethylenimine's transfer of plasmid DNA into mammalian cells
Open accessAbstract
Branched polyethylenimine (PEI) chains with an average molecular mass of 2 kDa (PEI2) have been covalently attached to gold nanoparticles (GNPs), and the potency of the resulting PEI2–GNPs conjugates as vectors for the delivery of plasmid DNA into monkey kidney (COS-7) cells in the presence of serum in vitro has been systematically investigated. The transfection efficiencies vary as a function of the PEI/gold molar ratio in the conjugates, with the best one (PEI2–GNPII) being 12 times more potent than the unmodified polycation. This potency can be further doubled by adding amphiphilic N -dodecyl–PEI2 during complex formation with DNA. The resulting ternary complexes are at least 1 order of magnitude more efficient than the 25-kDa PEI, one of the premier polycationic gene-delivery vectors. Importantly, although unmodified PEI2 transfects just 4% of the cells, PEI2–GNPII transfects 25%, and the PEI2–GNPII/dodecyl–PEI2 ternary complex transfects 50% of the cells. The intracellular trafficking of the DNA complexes of these vectors, monitored by transmission electron microscopy, has detected the complexes in the nucleus <1 h after transfection.
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Enhancing polyethylenimine's delivery of plasmid DNA into mammalian cells
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Self-citationNon-viral gene therapy: polycation-mediated DNA delivery
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Metadata
- Authors
- Thomas, M., & Klibanov, A. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-07-28
- Volume
- 100
- Issue
- 16
- Pages
- 9138-9143
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1994025696
- Cited by
- 549
- References
- 34
- FWCI
- 4.18
- Type
- journal article
- Language
- en
- Open access
- green
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