The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm
Open accessAbstract
Biofilms represent the predominant mode of microbial growth in the natural environment. Bacillus subtilis is a ubiquitous Gram-positive soil bacterium that functions as an effective plant growth-promoting agent. The biofilm matrix is composed of an exopolysaccharide and an amyloid fiber-forming protein, TasA, and assembles with the aid of a small secreted protein, BslA. Here we show that natively synthesized and secreted BslA forms surface layers around the biofilm. Biophysical analysis demonstrates that BslA can self-assemble at interfaces, forming an elastic film. Molecular function is revealed from analysis of the crystal structure of BslA, which consists of an Ig-type fold with the addition of an unusual, extremely hydrophobic “cap” region. A combination of in vivo biofilm formation and in vitro biophysical analysis demonstrates that the central hydrophobic residues of the cap are essential to allow a hydrophobic, nonwetting biofilm to form as they control the surface activity of the BslA protein. The hydrophobic cap exhibits physiochemical properties remarkably similar to the hydrophobic surface found in fungal hydrophobins; thus, BslA is a structurally defined bacterial hydrophobin. We suggest that biofilms formed by other species of bacteria may have evolved similar mechanisms to provide protection to the resident bacterial community.
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Cites
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The Jpred 3 secondary structure prediction server
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Microbial Extracellular Polymeric Substances (EPSs) in Ocean Systems
Decho, A. W., & Gutierrez, T. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00922 · Cited by 733
Hobley, L., Harkins, C., MacPhee, C. E., & Stanley-Wall, N. R. (2015) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuv015 · Cited by 581
“Tensiometry demonstrated that the recombinant BslA, while soluble in so-lution, was surface active meaning that the protein migrates to an air/water or oil/water interface where it forms an elastic film (Hobley et al. 2013).”
BackgroundMethodInfluentialSelf-citationBacillus subtilis biofilm formation and social interactions
Arnaouteli, S., Bamford, N. C., Stanley-Wall, N. R., & Kovács, Á. T. (2021) · Nature Reviews Microbiology · doi:10.1038/s41579-021-00540-9 · Cited by 532
Self-citationFlemming, H.-C. (2016) · Microorganisms · doi:10.3390/microorganisms4040041 · Cited by 374
Probiotic Bacillus subtilis protects against α-synuclein aggregation in C. elegans
Goya, M. E., Xue, F., Sampedro-Torres-Quevedo, C., Arnaouteli, S., Riquelme-Dominguez, L., Romanowski, A., Brydon, J., Ball, K. L., Stanley-Wall, N. R., & Doitsidou, M. (2020) · Cell Reports · doi:10.1016/j.celrep.2019.12.078 · Cited by 205
Self-citation
Metadata
- Authors
- Hobley, L., Ostrowski, A., Rao, F. V., Bromley, K. M., Porter, M., Prescott, A. R., MacPhee, C. E., van Aalten, D. M. F., & Stanley-Wall, N. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2013-07-31
- Volume
- 110
- Issue
- 33
- Pages
- 13600-13605
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2143160816
- Cited by
- 294
- References
- 47
- FWCI
- 9.68
- Type
- journal article
- Language
- en
- Open access
- green
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- Hobley, L., Ostrowski, A., Rao, F. V., Bromley, K. M., Porter, M., Prescott, A. R., MacPhee, C. E., van Aalten, D. M. F., & Stanley-Wall, N. R. (2013). BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1306390110
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