The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Dnmt2-dependent methylomes lack defined DNA methylation patterns
Open accessAbstract
Several organisms have retained methyltransferase 2 ( Dnmt2 ) as their only candidate DNA methyltransferase gene. However, information about Dnmt2-dependent methylation patterns has been limited to a few isolated loci and the results have been discussed controversially. In addition, recent studies have shown that Dnmt2 functions as a tRNA methyltransferase, which raised the possibility that Dnmt2 -only genomes might be unmethylated. We have now used whole-genome bisulfite sequencing to analyze the methylomes of Dnmt2 -only organisms at single-base resolution. Our results show that the genomes of Schistosoma mansoni and Drosophila melanogaster lack detectable DNA methylation patterns. Residual unconverted cytosine residues shared many attributes with bisulfite deamination artifacts and were observed at comparable levels in Dnmt2 -deficient flies. Furthermore, genetically modified Dnmt2 -only mouse embryonic stem cells lost the DNA methylation patterns found in wild-type cells. Our results thus uncover fundamental differences among animal methylomes and suggest that DNA methylation is dispensable for a considerable number of eukaryotic organisms.
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Human DNA methylomes at base resolution show widespread epigenomic differences
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EUKARYOTIC CYTOSINE METHYLTRANSFERASES
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Genome-Wide Evolutionary Analysis of Eukaryotic DNA Methylation
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Methylation of tRNA Asp by the DNA Methyltransferase Homolog Dnmt2
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Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation
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Transcriptome-wide distribution and function of RNA hydroxymethylcytosine
Delatte, B., Wang, F., Ngoc, L. V., Collignon, E., Bonvin, E., Deplus, R., Calonne, E., Hassabi, B., Putmans, P., Awe, S., et al. (2016) · Science · doi:10.1126/science.aac5253 · Cited by 411
Olova, N., Krueger, F., Andrews, S., Oxley, D., Berrens, R. V., Branco, M. R., & Reik, W. (2018) · Genome Biology · doi:10.1186/s13059-018-1408-2 · Cited by 313
Self-citationDNA methylation in social insects: how epigenetics can control behavior and longevity
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Identification and functional characterization of transcriptional activators in human cells
Alerasool, N., Leng, H., Lin, Z.-Y., Gingras, A.-C., & Taipale, M. (2022) · Molecular Cell · doi:10.1016/j.molcel.2021.12.008 · Cited by 120
Metadata
- Authors
- Raddatz, G., Guzzardo, P. M., Olova, N., Fantappié, M. R., Rampp, M., Schaefer, M., Reik, W., Hannon, G. J., & Lyko, F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2013-05-02
- Volume
- 110
- Issue
- 21
- Pages
- 8627-8631
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2159016980
- Cited by
- 257
- References
- 42
- FWCI
- 11.83
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Raddatz, G., Guzzardo, P. M., Olova, N., Fantappié, M. R., Rampp, M., Schaefer, M., Reik, W., Hannon, G. J., & Lyko, F. (2013). Dnmt2-dependent methylomes lack defined DNA methylation patterns. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1306723110
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