The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory
Open accessAbstract
Significance: We combined molecule counting and superresolution microscopy to observe the size, shape, and biomolecular composition of vesicles in the endocytic pathway. From a large number of steady-state snapshots, we follow the maturation of vesicles to endosomes. First, phosphatidylinositol 3-phosphate (PI3P) is generated on newly formed vesicles, principally after they shed their clathrin coat. Next, PI3P sites increase (∼100-fold) on small vesicles whose size is tightly regulated. Finally, the GTPase Vps21 (Rab5 homolog) is associated with small endosomal structures during the initial phase of PI3P production, whereas Ypt7 (Rab7 homolog) localizes to larger endosomal structures, which exhibit saturating levels of PI3P.This new technique opens up the possibility of accurately measuring molecular stoichiometries across several orders of magnitude while resolving subdiffraction-limit organelles.
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Stochastic Gene Expression in a Single Cell
Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002) · Science · doi:10.1126/science.1070919 · Cited by 5,441
Global analysis of protein expression in yeast
Ghaemmaghami, S., Huh, W.-K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., & Weissman, J. S. (2003) · Nature · doi:10.1038/nature02046 · Cited by 3,750
Phosphoinositides in cell regulation and membrane dynamics
Di Paolo, G., & De Camilli, P. (2006) · Nature · doi:10.1038/nature05185 · Cited by 2,790
Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise
Newman, J. R. S., Ghaemmaghami, S., Ihmels, J., Breslow, D. K., Noble, M., DeRisi, J. L., & Weissman, J. S. (2006) · Nature · doi:10.1038/nature04785 · Cited by 1,483
Stochastic protein expression in individual cells at the single molecule level
Cai, L., Friedman, N., & Xie, X. S. (2006) · Nature · doi:10.1038/nature04599 · Cited by 1,145 · cited 4×
“Burd CG, Emr SD (1998) Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains.”
BackgroundInfluentialRabs and their effectors: achieving specificity in membrane traffic
Grosshans, B. L., Ortiz, D., & Novick, P. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601617103 · Cited by 1,005
A Modular Design for the Clathrin- and Actin-Mediated Endocytosis Machinery
Kaksonen, M., Toret, C. P., & Drubin, D. G. (2005) · Cell · doi:10.1016/j.cell.2005.09.024 · Cited by 726
McBride, H. M., Rybin, V., Murphy, C., Giner, A., Teasdale, R., & Zerial, M. (1999) · Cell · doi:10.1016/s0092-8674(00)81966-2 · Cited by 520
Veatch, S. L., Machta, B. B., Shelby, S. A., Chiang, E. N., Holowka, D. A., & Baird, B. A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0031457 · Cited by 290
Clathrin-mediated endocytosis in budding yeast
Weinberg, J., & Drubin, D. G. (2012) · Trends in Cell Biology · doi:10.1016/j.tcb.2011.09.001 · Cited by 236 · cited 2×
“Formation of vesicles, tethering, fusion, and maturation to endosomes are controlled by over 60 proteins in concert with conversion of phosphoinositides (PIs) (4, 5).”
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Cited by
Super-resolution microscopy with DNA-PAINT
Schnitzbauer, J., Strauss, M. T., Schlichthaerle, T., Schueder, F., & Jungmann, R. (2017) · Nature Protocols · doi:10.1038/nprot.2017.024 · Cited by 1,047
Fluorescence nanoscopy in cell biology
Sahl, S. J., Hell, S. W., & Jakobs, S. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.71 · Cited by 990
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Wu, C.-Y., Roybal, K. T., Puchner, E. M., Onuffer, J., & Lim, W. A. (2015) · Science · doi:10.1126/science.aab4077 · Cited by 687
Self-citationSR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data
Levet, F., Hosy, E., Kechkar, A., Butler, C., Beghin, A., Choquet, D., & Sibarita, J.-B. (2015) · Nature Methods · doi:10.1038/nmeth.3579 · Cited by 502
Quantitative super-resolution imaging with qPAINT
Jungmann, R., Avendaño, M. S., Dai, M., Woehrstein, J. B., Agasti, S. S., Feiger, Z., Rodal, A., & Yin, P. (2016) · Nature Methods · doi:10.1038/nmeth.3804 · Cited by 420
Metadata
- Authors
- Puchner, E. M., Walter, J. M., Kasper, R., Huang, B., & Lim, W. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2013-09-16
- Volume
- 110
- Issue
- 40
- Pages
- 16015-16020
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2063096840
- Cited by
- 177
- References
- 10
- FWCI
- 11.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Puchner, E. M., Walter, J. M., Kasper, R., Huang, B., & Lim, W. A. (2013). Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1309676110
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