The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Dissecting the role of the Golgi complex and lipid rafts in biosynthetic transport of cholesterol to the cell surface
Open accessAbstract
In this study, we compared the transport of newly synthesized cholesterol with that of influenza virus hemagglutinin (HA) from the endoplasmic reticulum to the plasma membrane. The arrival of cholesterol on the cell surface was monitored by cyclodextrin removal, and HA transport was monitored by surface trypsinization and endoglycosidase H digestion. We found that disassembly of the Golgi complex by brefeldin A treatment resulted in partial inhibition of cholesterol transport while completely blocking HA transport. Further, microtubule depolymerization by nocodazole inhibited cholesterol and HA transport to a similar extent. When the partitioning of cholesterol into lipid rafts was analyzed, we found that newly synthesized cholesterol began to associate with low-density detergent-resistant membranes rapidly after synthesis, before it was detectable on the cell surface, and its raft association increased further upon chasing. When cholesterol transport was blocked by using 15°C incubation, the association of newly synthesized cholesterol with low-density detergent-insoluble membranes was decreased and cholesterol accumulated in a fraction with intermediate density. Our results provide evidence for the partial contribution of the Golgi complex to the transport of newly synthesized cholesterol to the cell surface and suggest that detergent-resistant membranes are involved in the process.
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Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
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Functional rafts in cell membranes
Simons, K., & Ikonen, E. (1997) · Nature · doi:10.1038/42408 · Cited by 9,757
Self-citationA Receptor-Mediated Pathway for Cholesterol Homeostasis
Brown, M. S., & Goldstein, J. L. (1986) · Science · doi:10.1126/science.3513311 · Cited by 5,703
Matlin, K. S., & Simons, K. (1984) · The Journal of cell biology · doi:10.1083/jcb.99.6.2131 · Cited by 253
Cited by
Lipid landscapes and pipelines in membrane homeostasis
Holthuis, J. C. M., & Menon, A. K. (2014) · Nature · doi:10.1038/nature13474 · Cited by 901
Cholesterol metabolism and homeostasis in the brain
Zhang, J., & Liu, Q. (2015) · Protein & Cell · doi:10.1007/s13238-014-0131-3 · Cited by 686
Lipid transfer proteins: the lipid commute via shuttles, bridges and tubes
Wong, L. H., Gatta, A. T., & Levine, T. P. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0071-5 · Cited by 505
Pol, A., Luetterforst, R., Lindsay, M., Heino, S., Ikonen, E., & Parton, R. G. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.152.5.1057 · Cited by 326
Self-citationIntracellular cholesterol transport
Soccio, R. E., & Breslow, J. L. (2004) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000131264.66417.d5 · Cited by 297
Metadata
- Authors
- Heino, S., Lusa, S., Somerharju, P., Ehnholm, C., Olkkonen, V. M., & Ikonen, E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-07-11
- Volume
- 97
- Issue
- 15
- Pages
- 8375-8380
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2072024204
- Cited by
- 254
- References
- 4
- FWCI
- 19.80
- Type
- journal article
- Language
- en
- Open access
- green
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- Heino, S., Lusa, S., Somerharju, P., Ehnholm, C., Olkkonen, V. M., & Ikonen, E. (2000). Dissecting the role of the Golgi complex and lipid rafts in biosynthetic transport of cholesterol to the cell surface. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.140218797
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