The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Improving fatty acids production by engineering dynamic pathway regulation and metabolic control
Open accessAbstract
Significance: One important synthetic chemistry reaction endowed by nature is the decarboxylative carbon condensation reaction using malonyl-CoA as carbon donor. Previous metabolic engineering efforts centered on the malonyl-CoA–dependent pathway have resulted in the production of many value-added compounds. Here we mimicked the native biological systems and used a dynamic regulatory network to optimize production titers and yield. The naturally existing transcriptional regulator FapR was rewired to dynamically control gene expressions involved in the supply and consumption of malonyl-CoA. Applying this metabolic control allowed the engineered cell to dynamically regulate pathway expression and compensated the metabolic activity of critical enzymes. The synthetic malonyl-CoA switch engineered in this study opens up new venues for dynamic pathway optimization and efficient production of malonyl-CoA–derived compounds.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Network motifs: simple building blocks of complex networks.
Milo, R., Shen-Orr, S., Itzkovitz, S., Kashtan, N., Chklovskii, D., & Alon, U. (2002) · Science · doi:10.1126/science.298.5594.824 · Cited by 7,368
Assessing computational tools for the discovery of transcription factor binding sites
Tompa, M., Li, N., Bailey, T. L., Church, G. M., De Moor, B., Eskin, E., Favorov, A. V., Frith, M. C., Fu, Y., Kent, W. J., et al. (2005) · Nature Biotechnology · doi:10.1038/nbt1053 · Cited by 1,268
Anthony, J. R., Anthony, L. C., Nowroozi, F., Kwon, G., Newman, J. D., & Keasling, J. D. (2009) · Metabolic Engineering · doi:10.1016/j.ymben.2008.07.007 · Cited by 292
Cited by
Gupta, A., Reizman, I. M. B., Reisch, C. R., & Prather, K. L. J. (2017) · Nature Biotechnology · doi:10.1038/nbt.3796 · Cited by 490
Xu, P., Qiao, K., Ahn, W. S., & Stephanopoulos, G. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1607295113 · Cited by 441
Self-citationBiomass-derived aviation fuels: Challenges and perspective
Wang, M., Dewil, R., Maniatis, K., Wheeldon, J., Tan, T., Baeyens, J., & Fang, Y. (2019) · Progress in Energy and Combustion Science · doi:10.1016/j.pecs.2019.04.004 · Cited by 331
Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals
Yan, Q., & Pfleger, B. F. (2020) · Metabolic Engineering · doi:10.1016/j.ymben.2019.04.009 · Cited by 105
Xia, Y., Du, X., Liu, B., Guo, S., & Huo, Y.-X. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae429 · Cited by 31
Metadata
- Authors
- Xu, P., Li, L., Zhang, F., Stephanopoulos, G., & Koffas, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2014-07-21
- Volume
- 111
- Issue
- 31
- Pages
- 11299-11304
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2161782059
- Cited by
- 487
- References
- 3
- FWCI
- 18.62
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Improving fatty acids production by engineering dynamic pathway regulation and metabolic control. https://app.cochat.ai/papers/10.1073/pnas.1406401111
- The paper
- Xu, P., Li, L., Zhang, F., Stephanopoulos, G., & Koffas, M. (2014). Improving fatty acids production by engineering dynamic pathway regulation and metabolic control. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1406401111
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
