The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Quantitative and stoichiometric analysis of the microRNA content of exosomes
Open accessAbstract
Significance: Exosomes have been a subject of great interest in recent years, especially in the context of the microRNAs (miRNAs) that they contain. Exosome-mediated miRNA transfer between cells has been proposed to be a mechanism for intercellular signaling and exosome-associated miRNAs in biofluids have been suggested as potential minimally invasive biomarkers for multiple human disease states. Remarkably, we show here that most exosomes derived from standard preparations do not harbor many copies of miRNA molecules. These findings suggest a reevaluation of current models of the mechanism of exosome-mediated miRNA communication and indicate that stoichiometric analysis will be valuable for the study of other populations of extracellular vesicles and their associated RNAs as well.
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Cites
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Cited by
The biology , function , and biomedical applications of exosomes
Kalluri, R., & LeBleu, V. S. (2020) · Science · doi:10.1126/science.aau6977 · Cited by 10,770
“Although exosomal cargo analyses require large pools of purified exosomes, not all exosomes contain a similar abundance of a given cargo, as observed, for example, with miRNA exosomal cargo (22).”
BackgroundCommunication by Extracellular Vesicles: Where We Are and Where We Need to Go
Tkach, M., & Théry, C. (2016) · Cell · doi:10.1016/j.cell.2016.01.043 · Cited by 3,444
Regulation of microRNA function in animals
Gebert, L. F. R., & MacRae, I. J. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0045-7 · Cited by 2,284
Extracellular Vesicles in Cancer: Cell-to-Cell Mediators of Metastasis
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Exosome and Exosomal MicroRNA: Trafficking, Sorting, and Function
Zhang, J., Li, S., Li, L., Li, M., Guo, C., Yao, J., & Mi, S. (2015) · Genomics, Proteomics & Bioinformatics · doi:10.1016/j.gpb.2015.02.001 · Cited by 1,901
Metadata
- Authors
- Chevillet, J. R., Kang, Q., Ruf, I. K., Briggs, H. A., Vojtech, L. N., Hughes, S. M., Cheng, H. H., Arroyo, J. D., Meredith, E. K., Gallichotte, E. N., Pogosova-Agadjanyan, E. L., Morrissey, C., Stirewalt, D. L., Hladik, F., Yu, E. Y., Higano, C. S., & Tewari, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2014-09-29
- Volume
- 111
- Issue
- 41
- Pages
- 14888-14893
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2073341747
- Cited by
- 1,096
- References
- 10
- FWCI
- 28.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Chevillet, J. R., Kang, Q., Ruf, I. K., Briggs, H. A., Vojtech, L. N., Hughes, S. M., Cheng, H. H., Arroyo, J. D., Meredith, E. K., Gallichotte, E. N., Pogosova-Agadjanyan, E. L., Morrissey, C., Stirewalt, D. L., Hladik, F., Yu, E. Y., Higano, C. S., & Tewari, M. (2014). Quantitative and stoichiometric analysis of the microRNA content of exosomes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1408301111
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