Cited by 273Microbial Community Ecology and PhysiologyGenomics and Phylogenetic StudiesWastewater Treatment and Nitrogen Removal

Genomic and proteomic characterization of “ Candidatus Nitrosopelagicus brevis”: An ammonia-oxidizing archaeon from the open ocean

Open access
Santoro, Alyson E., Dupont, Christopher L., Richter, R. Alex, Craig, Matthew T., Carini, Paul, McIlvin, Matthew R., Yang, Youngik, Orsi, William D. +2 more·2015·Proceedings of the National Academy of Sciences

Abstract

Thaumarchaeota are among the most abundant microbial cells in the ocean, but difficulty in cultivating marine Thaumarchaeota has hindered investigation into the physiological and evolutionary basis of their success. We report here a closed genome assembled from a highly enriched culture of the ammonia-oxidizing pelagic thaumarchaeon CN25, originating from the open ocean. The CN25 genome exhibits strong evidence of genome streamlining, including a 1.23-Mbp genome, a high coding density, and a low number of paralogous genes. Proteomic analysis recovered nearly 70% of the predicted proteins encoded by the genome, demonstrating that a high fraction of the genome is translated. In contrast to other minimal marine microbes that acquire, rather than synthesize, cofactors, CN25 encodes and expresses near-complete biosynthetic pathways for multiple vitamins. Metagenomic fragment recruitment indicated the presence of DNA sequences >90% identical to the CN25 genome throughout the oligotrophic ocean. We propose the provisional name "Candidatus Nitrosopelagicus brevis" str. CN25 for this minimalist marine thaumarchaeon and suggest it as a potential model system for understanding archaeal adaptation to the open ocean.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1073/pnas.1416223112

Cites

64 of 64 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • These tradeoffs include replacing Fe-containing superoxide dismutase with a Ni-containing version, exchanging an Fe protein with a Cu protein in photosynthesis and ammonia oxidation, and using B12 instead of Fe for ribonucleotide reductase.

    “These tradeoffs include the replacement of a Fe-containing superoxide dismutase with a Nicontaining version (Dupont et al., 2012), exchanging an Fe protein with a Cu protein in the electron transport chains in photosynthesis (Peers and Price, 2006) and ammonia oxidation (Santoro et al., 2015), and using B12 instead of Fe for ribonucleotide reductase.”

    From Trace elements at the intersection of marine biological and geochemical evolution (2016) · cites this paper as Santoro et al., 2015

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 2

  1. Spang A, et al. (2012) The genome of the ammonia-oxidizing Candidatus Nitro- sosphaera gargensis: Insights into metabolic versatility and environmental adapta- tions. Environ Microbiol 14(12):3122-3145.
  2. Graupner M, White RH (2001) Methanococcus jannaschii generates L-proline by cy- clization of L-ornithine. J Bacteriol 183(17):5203-5205.

Cited by

Sorted by citations

Authors
Santoro, A. E., Dupont, C. L., Richter, R. A., Craig, M. T., Carini, P., McIlvin, M. R., Yang, Y., Orsi, W. D., Moran, D. M., & Saito, M. A.
Affiliations
  • University of Maryland Center for Environmental Science (US)
  • Microbial and Environmental Genomics and
  • J. Craig Venter Institute (US)
  • University of San Diego (US)
  • Woods Hole Oceanographic Institution (US)
Venue
Proceedings of the National Academy of Sciences
Published
2015-01-13
Volume
112
Issue
4
Pages
1173-1178
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2032670022
Cited by
268
References
64
Citations by year
2026: 12 · 2025: 22 · 2024: 23 · 2023: 23 · 2022: 26 · 2021: 25 · 2020: 30 · 2019: 31 · 2018: 21 · 2017: 18 · 2016: 25 · 2015: 12
FWCI
22.97
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
Gordon and Betty Moore Foundation (GBMF3307, GBMF3782, GBMF2724)
Topics
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
Keywords
ammonia-oxidizing archaea, Thaumarchaeota, marine archaea, genome streamlining, minimal genome, vitamin biosynthesis, cofactor biosynthesis, proteome, genome assembly
MeSH terms
Amino Acid Sequence, Archaea, Molecular Sequence Data, Oceans and Seas, Water Microbiology, Archaeal Proteins, Gene Expression Regulation, Archaeal, Proteome, Proteomics, Metagenomics

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review