Activation of mitochondrial protease OMA1 by Bax and Bak promotes cytochrome c release during apoptosis
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Significance: The release of cytochrome c from its normal intermembrane space in mitochondria marks the initiation of apoptosis in mammalian cells. The process is triggered by the aggregation of B-cell leukemia/lymphoma 2 (BCL2)-associated X (Bax) and BCL2-antagonist/killer (Bak) proteins on the surface of mitochondria. We found that a mitochondrial inner membrane protease, OMA1 (overlapping activity with m -AAA protease), is specifically activated and is responsible for cleaving another inner membrane protein, optical nerve atrophy 1 (OPA1), upon Bax/Bak aggregation. The cleavage of OPA1 triggers the remodeling of mitochondrial cristae, allowing the majority of cytochrome c inside the cristae to be released. This finding provided a more comprehensive understanding of this critical molecular event during apoptosis.
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The release of CYCS and SMAC to the cytosol is favored by mitochondrial cristae remodeling, which relies on the oligomerization and activation of OPA1, possibly preceded by BAX-dependent and BAK-dependent activation of OMA1 and/or DRP1.
“The release of CYCS and SMAC to the cytosol is favored by mitochondrial cristae remodeling [164], which relies on the oligomerization and activation of OPA1, mitochondrial dynamin like GTPase (OPA1) [165], possibly preceded by the BAX-dependent and BAK-dependent activation of OMA1 zinc metallopeptidase (OMA1) [166, 167], and/or dynamin 1 like (DNM1L; best known as DRP1) [168].”
From Molecular mechanisms of cell death: recommendations of the nomenclature committee on cell death 2018. (2018) · cites this paper as [166]
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- Authors
- Jiang, X., Jiang, H., Shen, Z., & Wang, X.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- National Institute of Biological Sciences, Beijing (CN)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2014-10
- Volume
- 111
- Issue
- 41
- Pages
- 14782-14787
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4205663
- OpenAlex
- W2080115272
- Cited by
- 220
- References
- 36
- Citations by year
- 2026: 7 · 2025: 17 · 2024: 17 · 2023: 18 · 2022: 21 · 2021: 24 · 2020: 24 · 2019: 24 · 2018: 17 · 2017: 20 · 2016: 18 · 2015: 11 · 2014: 2
- FWCI
- 6.97
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- OMA1, Bax, Bak, cytochrome c release, apoptosis, building information modeling, tBid, OPA1, mitochondrial cristae remodeling, mitochondrial outer membrane permeabilization, Bcl-2 family proteins
- MeSH terms
- Enzyme Activation, Humans, Metalloendopeptidases, Mitochondria, Apoptosis, GTP Phosphohydrolases, Cytochromes c, Cell Line, Tumor, bcl-2 Homologous Antagonist-Killer Protein, BH3 Interacting Domain Death Agonist Protein, bcl-2-Associated X Protein, Protein Stability, Gene Knockdown Techniques
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