The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Multicolor CRISPR labeling of chromosomal loci in human cells
Open accessAbstract
Significance: The detection of specific genes in fixed cells was first accomplished in 1969 by Gall and Pardue. The development of analogous methods applicable to living cells is now at hand. At the forefront of this advance (2013–2014), we and other investigators have used transcription activator-like effectors (TALEs) conjugated with fluorescent proteins to tag genomic loci in live cells. More recently, the CRISPR/Cas9 system has provided a more flexible approach to targeting specific loci. In this paper, we describe the labeling of human genomic loci in live cells with three orthogonal CRISPR/Cas9 components, allowing multicolor detection of genomic loci with high spatial resolution, which provides an avenue for barcoding elements of the human genome in the living state.
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Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
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<tt>BLAT</tt> —The <tt>BLAST</tt> -Like Alignment Tool
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Cited by
The CRISPR tool kit for genome editing and beyond
Adli, M. (2018) · Nature Communications · doi:10.1038/s41467-018-04252-2 · Cited by 1,545
The next generation of CRISPR–Cas technologies and applications
Pickar-Oliver, A., & Gersbach, C. A. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0131-5 · Cited by 1,459
Delivering CRISPR: a review of the challenges and approaches
Lino, C. A., Harper, J. C., Carney, J. P., & Timlin, J. A. (2018) · Drug Delivery · doi:10.1080/10717544.2018.1474964 · Cited by 1,185
CRISPR/Cas9 in Genome Editing and Beyond
Wang, H., La Russa, M., & Qi, L. S. (2016) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060815-014607 · Cited by 1,110
CRISPR/Cas Systems towards Next-Generation Biosensing
Li, Y., Li, S., Wang, J., & Liu, G. (2019) · Trends in Biotechnology · doi:10.1016/j.tibtech.2018.12.005 · Cited by 917
Metadata
- Authors
- Ma, H., Naseri, A., Reyes-Gutierrez, P., Wolfe, S. A., Zhang, S., & Pederson, T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2015-02-23
- Volume
- 112
- Issue
- 10
- Pages
- 3002-3007
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2043890495
- Cited by
- 412
- References
- 8
- FWCI
- 22.51
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Ma, H., Naseri, A., Reyes-Gutierrez, P., Wolfe, S. A., Zhang, S., & Pederson, T. (2015). Multicolor CRISPR labeling of chromosomal loci in human cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1420024112
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