The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Nef increases the synthesis of and transports cholesterol to lipid rafts and HIV-1 progeny virions
Open accessAbstract
HIV buds from lipid rafts and requires cholesterol for its egress from and entry into cells. Viral accessory protein Nef plays a major role in this process. In this study, it not only increased the biosynthesis of lipid rafts and viral particles with newly synthesized cholesterol, but also enriched them. Furthermore, via the consensus cholesterol recognition motif at its C terminus, Nef bound cholesterol. When this sequence was mutated, Nef became unable to transport newly synthesized cholesterol into lipid rafts and viral particles. Interestingly, although its levels in lipid rafts were not affected, this mutant Nef protein was poorly incorporated into viral particles, and viral infectivity decreased dramatically. Thus, Nef also transports newly synthesized cholesterol to the site of viral budding. As such, it provides essential building blocks for the formation of viruses that replicate optimally in the host.
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Cites
A Receptor-Mediated Pathway for Cholesterol Homeostasis
Brown, M. S., & Goldstein, J. L. (1986) · Science · doi:10.1126/science.3513311 · Cited by 5,703
Li, H., Yao, Z.-X., Degenhardt, B., Teper, G., & Papadopoulos, V. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.3.1267 · Cited by 317
Romanowski, M. J., Soccio, R. E., Breslow, J. L., & Burley, S. K. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.052140699 · Cited by 162
Virion-associated cholesterol is critical for the maintenance of HIV-1 structure and infectivity
Campbell, S. M., Crowe, S. M., & Mak, J. (2002) · AIDS · doi:10.1097/00002030-200211220-00004 · Cited by 139
Cited by
Species-Specific Activity of SIV Nef and HIV-1 Vpu in Overcoming Restriction by Tetherin/BST2
Jia, B., Serra-Moreno, R., Neidermyer, W., Rahmberg, A., Mackey, J., Fofana, I. B., Johnson, W. E., Westmoreland, S., & Evans, D. T. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000429 · Cited by 406
Multifaceted roles for lipids in viral infection
Heaton, N. S., & Randall, G. (2011) · Trends in Microbiology · doi:10.1016/j.tim.2011.03.007 · Cited by 381
Human Immunodeficiency Virus Impairs Reverse Cholesterol Transport from Macrophages
Mujawar, Z., Rose, H., Morrow, M. P., Pushkarsky, T., Dubrovsky, L., Mukhamedova, N., Fu, Y., Dart, A., Orenstein, J. M., Bobryshev, Y. V., et al. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040365 · Cited by 335
Statins Inhibit HIV-1 Infection by Down-regulating Rho Activity
del Real, G., Jiménez-Baranda, S., Mira, E., Lacalle, R. A., Lucas, P., Gómez-Moutón, C., Alegret, M., Peña, J. M., Rodríguez-Zapata, M., Alvarez-Mon, M., et al. (2004) · The Journal of Experimental Medicine · doi:10.1084/jem.20040061 · Cited by 294
Role of viral regulatory and accessory proteins in HIV-1 replication
Seelamgari, A. (2004) · Frontiers in Bioscience · doi:10.2741/1403 · Cited by 98
Metadata
- Authors
- Zheng, Y.-H., Plemenitas, A., Fielding, C. J., & Peterlin, B. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-06-24
- Volume
- 100
- Issue
- 14
- Pages
- 8460-8465
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2012690743
- Cited by
- 179
- References
- 4
- FWCI
- 4.19
- Type
- journal article
- Language
- en
- Open access
- green
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- Zheng, Y.-H., Plemenitas, A., Fielding, C. J., & Peterlin, B. M. (2003). Nef increases the synthesis of and transports cholesterol to lipid rafts and HIV-1 progeny virions. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1437453100
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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