The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM
Open accessAbstract
Significance: The systematic discovery of new gene and drug combinations that modulate complex biological phenotypes and human diseases requires scalable and multiplexed screening technologies. We leverage the programmability of the CRISPR-Cas9 system for multiplexed targeting of specific genomic loci and the versatility of the combinatorial genetics en masse (CombiGEM) technology to rapidly assemble barcoded combinatorial genetic perturbation libraries that can be tracked with high-throughput sequencing. CombiGEM-CRISPR enables simple, massively parallel screening of barcoded combinatorial gene perturbations in human cells, and the translation of these hits into effective drug combinations. This approach is broadly applicable for performing pooled combinatorial genetic perturbations to map out how the orchestrated action of genes controls complex phenotypes and to translate these findings into novel drug combinations.
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Citations
Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
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Genome engineering using the CRISPR-Cas9 system
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RNA-Guided Human Genome Engineering via Cas9
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“In addition to generating gene knockouts, the CombiGEM-CRISPR platform could be used for high-order combinatorial gene activation and repression studies by incorporating gRNAs and deactivated Cas9 variants repurposed as transcriptional and epigenetic regulators (8, 9, 51, 52).”
MethodGenome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.029 · Cited by 2,728
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Cited by
Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
Joung, J., Konermann, S., Gootenberg, J. S., Abudayyeh, O. O., Platt, R. J., Brigham, M. D., Sanjana, N. E., & Zhang, F. (2017) · Nature Protocols · doi:10.1038/nprot.2017.016 · Cited by 1,243
Horlbeck, M. A., Gilbert, L. A., Villalta, J. E., Adamson, B., Pak, R. A., Chen, Y., Fields, A. P., Park, C. Y., Corn, J. E., Kampmann, M., et al. (2016) · eLife · doi:10.7554/elife.19760 · Cited by 894
Past, present, and future of CRISPR genome editing technologies
Pacesa, M., Pelea, O., & Jinek, M. (2024) · Cell · doi:10.1016/j.cell.2024.01.042 · Cited by 609
Am I ready for CRISPR? A user's guide to genetic screens
Doench, J. G. (2018) · Nature Reviews Genetics · doi:10.1038/nrg.2017.97 · Cited by 452
Exploring genetic interaction manifolds constructed from rich single-cell phenotypes
Norman, T. M., Horlbeck, M. A., Replogle, J. M., Ge, A. Y., Xu, A., Jost, M., Gilbert, L. A., & Weissman, J. S. (2019) · Science · doi:10.1126/science.aax4438 · Cited by 392
Metadata
- Authors
- Wong, A. S. L., Choi, G. C. G., Cui, C. H., Pregernig, G., Milani, P., Adam, M., Perli, S. D., Kazer, S. W., Gaillard, A., Hermann, M., Shalek, A. K., Fraenkel, E., & Lu, T. K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2016-02-10
- Volume
- 113
- Issue
- 9
- Pages
- 2544-2549
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2271163371
- Cited by
- 242
- References
- 23
- FWCI
- 15.80
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Wong, A. S. L., Choi, G. C. G., Cui, C. H., Pregernig, G., Milani, P., Adam, M., Perli, S. D., Kazer, S. W., Gaillard, A., Hermann, M., Shalek, A. K., Fraenkel, E., & Lu, T. K. (2016). Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1517883113
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